Incessant transitions between active and silent states in cortico-thalamic circuits and altered neuronal excitability lead to epilepsy

La ligne directrice de nos expériences a été l'hypothèse que l'apparition et/ou la persistance des fluctuations de longue durée entre les états silencieux et actifs dans les réseaux néocorticaux et une excitabilité neuronale modifiée sont les facteurs principaux de l'épileptogenèse, menant aux crises d’épilepsie avec expression comportementale. Nous avons testé cette hypothèse dans deux modèles expérimentaux différents. La déafférentation corticale chronique a essayé de répliquer la déafférentation physiologique du neocortex observée pendant le sommeil à ondes lentes. Dans ces conditions, caractérisées par une diminution de la pression synaptique et par une incidence augmentée de périodes silencieuses dans le système cortico-thalamique, le processus de plasticité homéostatique augmente l’excitabilité neuronale. Par conséquent, le cortex a oscillé entre des périodes actives et silencieuses et, également, a développé des activités hyper-synchrones, s'étendant de l’hyperexcitabilité cellulaire à l'épileptogenèse focale et à des crises épileptiques généralisées. Le modèle de stimulation sous-liminale chronique (« kindling ») du cortex cérébral a été employé afin d'imposer au réseau cortical une charge synaptique supérieure à celle existante pendant les états actifs naturels - état de veille ou sommeil paradoxal (REM). Dans ces conditions un mécanisme différent de plasticité qui s’est exprimé dans le système thalamo-corticale a imposé pour des longues périodes de temps des oscillations continuelles entre les époques actives et silencieuses, que nous avons appelées des activités paroxysmiques persistantes. Indépendamment du mécanisme sous-jacent de l'épileptogenèse les crises d’épilepsie ont montré certaines caractéristiques similaires : une altération dans l’excitabilité neuronale mise en évidence par une incidence accrue des décharges neuronales de type bouffée, une tendance constante vers la généralisation, une propagation de plus en plus rapide, une synchronie augmentée au cours du temps, et une modulation par les états de vigilance (facilitation pendant le sommeil à ondes lentes et barrage pendant le sommeil REM). Les états silencieux, hyper-polarisés, de neurones corticaux favorisent l'apparition des bouffées de potentiels d’action en réponse aux événements synaptiques, et l'influence post-synaptique d'une bouffée de potentiels d’action est beaucoup plus importante par rapport à l’impacte d’un seul potentiel d’action. Nous avons également apporté des évidences que les neurones néocorticaux de type FRB sont capables à répondre avec des bouffées de potentiels d’action pendant les phases hyper-polarisées de l'oscillation lente, propriété qui peut jouer un rôle très important dans l’analyse de l’information dans le cerveau normal et dans l'épileptogenèse. Finalement, nous avons rapporté un troisième mécanisme de plasticité dans les réseaux corticaux après les crises d’épilepsie - une diminution d’amplitude des potentiels post-synaptiques excitatrices évoquées par la stimulation corticale après les crises - qui peut être un des facteurs responsables des déficits comportementaux observés chez les patients épileptiques. Nous concluons que la transition incessante entre des états actifs et silencieux dans les circuits cortico-thalamiques induits par disfacilitation (sommeil à ondes lentes), déafférentation corticale (épisodes ictales à 4-Hz) ou par une stimulation sous-liminale chronique (activités paroxysmiques persistantes) crée des circonstances favorables pour le développement de l'épileptogenèse. En plus, l'augmentation de l’incidence des bouffées de potentiels d’actions induisant une excitation post-synaptique anormalement forte, change l'équilibre entre l'excitation et l'inhibition vers une supra-excitation menant a l’apparition des crises d’épilepsie.The guiding line in our experiments was the hypothesis that the occurrence and / or the persistence of long-lasting fluctuations between silent and active states in the neocortical networks, together with a modified neuronal excitability are the key factors of epileptogenesis, leading to behavioral seizures. We addressed this hypothesis in two different experimental models. The chronic cortical deafferentation replicated the physiological deafferentation of the neocortex observed during slow-wave sleep (SWS). Under these conditions of decreased synaptic input and increased incidence of silent periods in the corticothalamic system the process of homeostatic plasticity up-regulated cortical cellular and network mechanisms and leaded to an increased excitability. Therefore, the deafferented cortex was able to oscillate between active and silent epochs for long periods of time and, furthermore, to develop highly synchronized activities, ranging from cellular hyperexcitability to focal epileptogenesis and generalized seizures. The kindling model was used in order to impose to the cortical network a synaptic drive superior to the one naturally occurring during the active states - wake or rapid eye movements (REM) sleep. Under these conditions a different plasticity mechanism occurring in the thalamo-cortical system imposed long-lasting oscillatory pattern between active and silent epochs, which we called outlasting activities. Independently of the mechanism of epileptogenesis seizures showed some analogous characteristics: alteration of the neuronal firing pattern with increased bursts probability, a constant tendency toward generalization, faster propagation and increased synchrony over the time, and modulation by the state of vigilance (overt during SWS and completely abolished during REM sleep). Silent, hyperpolarized, states of cortical neurons favor the induction of burst firing in response to depolarizing inputs, and the postsynaptic influence of a burst is much stronger as compared to a single spike. Furthermore, we brought evidences that a particular type of neocortical neurons - fast rhythmic bursting (FRB) class - is capable to consistently respond with bursts during the hyperpolarized phase of the slow oscillation, fact that may play a very important role in both normal brain processing and in epileptogenesis. Finally, we reported a third plastic mechanism in the cortical network following seizures - a decreasing amplitude of cortically evoked excitatory post-synaptic potentials (EPSP) following seizures - which may be one of the factors responsible for the behavioral deficits observed in patients with epilepsy. We conclude that incessant transitions between active and silent states in cortico-thalamic circuits induced either by disfacilitation (sleep), cortical deafferentation (4-Hz ictal episodes) and by kindling (outlasting activities) create favorable circumstances for epileptogenesis. The increase in burst-firing, which further induce abnormally strong postsynaptic excitation, shifts the balance of excitation and inhibition toward overexcitation leading to the onset of seizures

Gridovi fine prostorne rezolucije dnevnih visina snijega za Rumunjsku (2005.–2015.)

This study presents the spatial interpolation procedure from snow depth measurements at weather stations implying the following stages: (1) Spatial interpolation at 1 km × 1 km resolution of the mean multiannual values (2005-2015) corresponding to each month, computed from the data extracted from the climatological database; (2) Computation of the daily deviations against the multiannual monthly mean for every day and year over 2005–2015 and their spatial interpolation; (3) Spatio-temporal datasets were obtained through merging the two surfaces obtained in stages 1 and 2. The anomalies were considered to be the ratio between the daily snow depth values and the climatology. The spatial variability of the data used in the first stage was accounted for through the use of a series of predictors derived from the digital elevation model (DEM). To plot the maps with the climatological normals (multiannual means), the Regression-Kriging (RK) spatial interpolation method was used. In order to choose the optimum method applied in spatializing deviations, four interpolation methods were tested using a cross-validation procedure: Multiquadratic, Ordinary Kriging (separated and pooled variograms) and 3d Kriging.Ova studija prikazuje proceduru prostorne interpolacije mjerenja dubine snijega na meteorološkim postajama koja podrazumijeva sljedeće faze: (1) prostorna interpolacija pri rezoluciji od 1 km x 1 km srednjih višegodišnjih vrijednosti (2005.–2015.), koja se provodi s podacima iz klimatološke baze; (2) izračunavanje dnevnih odstupanja od višegodišnjeg mjesečnog srednjaka za svaki dan i godinu tijekom razdoblja od 2005. do 2015. i njihova prostorna interpolacija; (3) prostorno-vremenski skup podataka dobiven je združivanjem procjena dobivenih u fazi 1 i 2. Odstupanja su definirana kao omjeri dnevnih vrijednosti dubine snijeg i klimatološkog srednjaka. Prostorna varijabilnost podataka korištenih u prvoj fazi objašnjena je korištenjem niza prediktora izvedenih iz digitalnog modela visina (DEM). Karte klimatoloških normala (višegodišnji srednjaci) izrađene su metodom prostorne interpolacije zvanom regresijski kriging (RK). Za odabir optimalne metode za prostornu interpolaciju odstupanja, testirane su četiri metode interpolacije i ocijenjene pomoću postupka poprečne validacije: multikvadratična, obični kriging (razdvojeni i skupni variogrami) i 3D kriging

How does a medical team in the Oncology Department react to the Covid-19 pandemic?

The Covid-19 pandemic was and still is a great challenge for the entire world population. People in the first line, among them doctors being a very important category, faced the risk of disease and, in some cases, even became infected. The emotional consequences of this risk are highlighted in this paperwork which tries to stress, with the help of applied questionnaires, the presence of psycho-emotional disorders among the medical staff of the Oncology Department of St. Luke\u27s Hospital, Bucharest, during the pandemic. The medical staff had a low level of stress, adapting to the evolution of the pandemic to some extent . The main fear was that of illness and the most common emotional states experienced by the subjects were: worry, nervousness and dissatisfaction. Most of the medical staff showed negative emotions, but there were also people who felt positive emotions of relaxation, calmness or joy. The most common ways of managing the stress caused by the pandemic were: communicating with colleagues, family and friends, watching movies and enjoying various activities with the family at home

Variability and Change in Water Cycle at the Catchment Level

This study proposes a simple methodology for assessing future-projected evolution of water cycle components (precipitation, potential evapotranspiration, and potential runoff) based on the two-level Palmer model of the soil and their impact on drought conditions at basin level. The Palmer Drought Severity Index (PDSI) is used as drought metric. The catchments of rivers Arges, Mures, Prut, Siret and Somes (mid- and lower Danube basin) have been chosen as case studies. The present climate data consist of Romanian gridded dataset, monthly precipitation and values of streamflow from Romania and Republic of Moldova and potential evapotranspiration-related data from the Climate Research Unit (University of East Anglia). We used as future projections five numerical experiments with regional models obtained through the EURO-CORDEX initiative, under two Representative Concentration Pathway scenarios. The correlations between observed streamflow at the river basin outlets and PDSI-related components of the water cycle show that PDSI represents reasonably well processes taking place in the selected catchments. Depending on the specific scenario and catchment, droughts that in the Palmer classification were deemed as incipient, mild or severe under present climate will become a normal summer feature toward the end of this century, especially over catchments situated in the lower Danube basin

HEALTHY LANDSCAPES: A REVIEW OF THE RESEARCH ON URBAN LANDSCAPES ASSOCIATED WITH HEALTH AND WELLBEING

Cities shelter more than two thirds of the world’s population, and health security in such environments became a challenge. The outbreak of the SARS-COV-2 pandemic revealed the urgency of assessing urban resilience towards major health crises. Major cities are acknowledged for decreasing the health status of their residents through complex drivers, and researchers from various domains have been addressing these issues for the past three decades. The aim of our study focused on highlighting the main methods and indicators used by scholars to assess the impact of urban landscapes on health, and to cluster urban landscapes based on their conclusions. We scoped the scientific literature published in the past 10 years, addressing the issue of health in relation with urban landscapes. We used statistical approaches, API algorithms, and social network analysis for generating and exposing our results. Most studies focused on perception analyses (mainly self-perceived health), literature reviews or environmental quality impacts on health. Green and blue features were considered therapeutic landscapes, while dense built-up spaces were described as harmful. Urban landscapes are acknowledged as enablers or disablers of health, thus planning strategies and regulations should consider the impacts generated by the design and structure of new urban fabrics

Growth and Adaptive Capacity of Douglas Fir Genetic Resources from Western Romania under Climate Change

The most recent climate change scenarios show that Southern and Eastern Europe will be affected by a significant increase in temperature and drought frequency by the end of the 21st century. Romania has already recorded very high temperatures and long periods of drought over recent decades, the most affected regions being the south, west and east of the country. Considering that successful forest management requires suitable species and high-quality reproductive material for reforestation, the aim of this study was to evaluate and compare the growth and drought response of Douglas fir (Pseudotsuga menziessi var. menziesii) and Norway spruce (Picea abies) in two stands installed at the end of the 19th century in western Romania. The growth traits, wood characteristics and drought parameters (resistance, recovery, resilience and relative resilience) of Douglas fir and Norway spruce trees have been analyzed and compared. The climate–growth relationship was determined using growth response functions over the period 1938–2017. Additionally, to simulate the potential impact of climate change on Douglas fir in this region, the RCP4.5 scenario was used over two periods: 2041–2070 and 2071–2100. The results reveal that Douglas fir has an exceptional growth capacity, overcoming the Norway spruce since the early ages in both site conditions. The highest growth performances were seen in the low-productivity site. From analyzing the responses to drought events, considerable differences were found between species. The results highlight the high resistance and relative resilience to extreme droughts of Douglas fir compared to Norway spruce. However, autumn–winter temperatures play an important role in the adaptation of Douglas fir to site conditions in Romania. The use of appropriate provenances of Douglas fir in mixed stands with native broadleaved species may be an option for climatically exposed sites, thus increasing the value of these stands

Climate Change in the Provenance Regions of Romania over the Last 70 Years: Implications for Forest Management

The recent climate change scenarios show significant increases in temperature and extreme drought events in Southern and Eastern Europe by the end of the 21st century, which will have a serious impact on forest growth and adaptation, and important consequences for forest management. The system of provenance regions, according to the OECD Scheme and EU Directive, was thought to encourage the use of the local seed sources, under the concept ‘local is the best’. However, climate is changing faster than some species or populations can adapt or migrate, which raises some uncertainties with respect to the future performance of local populations. In Romania, as in other countries, the delimitation of provenance regions is based on geographical, ecological and vegetation criteria. The aim of this study is to evaluate: (1) the climate change that has occurred at the level of the provenance regions; (2) which regions will be most vulnerable to climate change; (3) which forest types will be the most vulnerable in a certain region; and (4) changes in the climatic envelope of forest species. Several climatic parameters and an ecoclimatic indices have been calculated and analyzed at the level of provenance regions, subregions and ecological sectors (forest types) in Romania, during the period 1951–2020. The results highlight a general shift towards warmer and drier conditions in the last 30 years, the mean annual temperature increasing with 0.3–1.1 °C across the provenance subregions. The De Martonne aridity index for the vegetation season shows that 86% of the ecological sectors fell into the arid and semiarid categories, which indicates a very high degree of vulnerability for forest species. On the Lang rainfall index, forest steppe climatic conditions occurred in all pure or mixed pedunculate oak forests, thermophile oak species, meadow forests, poplar and willow, Turkey oak and Hungarian oak forests. The Ellenberg coefficient highlights that the warming process is more evident along the altitude and the degree of vulnerability increase at lower altitude or at the edge of species distribution. The climate envelopes of many forest species have already shifted to another ecosystem’s climate. This paper presents the importance of re-delineation the provenance regions for the production and deployment of forest reproductive materials according to the climate change occurred in the last decades, as a fundamental tool for an adaptive forest management

Forests dynamics in the montane-alpine boundary: a comparative study using satellite imagery and climate data

14 páginas.- Ilustraciones.- © The authors 2017. Open Access under Creative Commons by Attribution Licence. Use, distribution and reproduction are unrestricted. Authors and original publication must be credited.Over the past decades, the altitudinal and latitudinal advance of forest lines has increased due to global warming and the abandonment of less productive areas previously subject to agricultural activities. The intensity and speed of the forest line advance also depend on numerous physical, biological and human factors that are region-specific. It is important to fully understand the mechanisms behind forest line behaviour, as existing studies do not report global figures. We selected 4 study areas in which to analyse the temporal and spatial behaviour of the forest line and of forest cover based on selection criteria such as minimal human interference and maximal representativeness at the European level. The sites were located in national parks that were evenly spread across some of the dominant European mountain ranges such as the Pyrenees, Alps and Carpathians, at comparable altitudes and latitudes, and with similar land cover proportions in the year 1970. Methodologically, we used cloud-free Landsat satellite images that were acquired in the same month during the growing season. A post-classification comparison technique, using all bands but the thermal one, was implemented to evaluate forest line behaviour, while the accuracy of image classification was evaluated by random sampling. Four time frames were used to evaluate forest cover behaviour in relation to the non-forested areas: 1971-1980, 1981-1990, 1991-2000 and 2001-2014. Also, climate and topography data were included in this study, which enabled comparison and computation of dependence relations. Our results indicate significant differences between the analysed areas. For instance, for the same reference period (1981-1990), the greatest differences in terms of forest cover change were specific to the Austrian Alps (28%), whereas the lowest differences were those from the Spanish Pyrenees (1%). Similar forest line shifts were found in the Austrian Alps and in the Romanian Carpathians, whereas the lowest altitudinal advancement was specific to the Spanish Pyrenees. According to this study, the temperature trend could have significantly influenced tree line behaviour.This article is based on work from COST Action ES1203 SensFor, supported by COST (European Cooperation in Science and Technology, www.cost.eu).Peer reviewe

Climate Change in the Provenance Regions of Romania over the Last 70 Years: Implications for Forest Management

The recent climate change scenarios show significant increases in temperature and extreme drought events in Southern and Eastern Europe by the end of the 21st century, which will have a serious impact on forest growth and adaptation, and important consequences for forest management. The system of provenance regions, according to the OECD Scheme and EU Directive, was thought to encourage the use of the local seed sources, under the concept ‘local is the best’. However, climate is changing faster than some species or populations can adapt or migrate, which raises some uncertainties with respect to the future performance of local populations. In Romania, as in other countries, the delimitation of provenance regions is based on geographical, ecological and vegetation criteria. The aim of this study is to evaluate: (1) the climate change that has occurred at the level of the provenance regions; (2) which regions will be most vulnerable to climate change; (3) which forest types will be the most vulnerable in a certain region; and (4) changes in the climatic envelope of forest species. Several climatic parameters and an ecoclimatic indices have been calculated and analyzed at the level of provenance regions, subregions and ecological sectors (forest types) in Romania, during the period 1951–2020. The results highlight a general shift towards warmer and drier conditions in the last 30 years, the mean annual temperature increasing with 0.3–1.1 °C across the provenance subregions. The De Martonne aridity index for the vegetation season shows that 86% of the ecological sectors fell into the arid and semiarid categories, which indicates a very high degree of vulnerability for forest species. On the Lang rainfall index, forest steppe climatic conditions occurred in all pure or mixed pedunculate oak forests, thermophile oak species, meadow forests, poplar and willow, Turkey oak and Hungarian oak forests. The Ellenberg coefficient highlights that the warming process is more evident along the altitude and the degree of vulnerability increase at lower altitude or at the edge of species distribution. The climate envelopes of many forest species have already shifted to another ecosystem’s climate. This paper presents the importance of re-delineation the provenance regions for the production and deployment of forest reproductive materials according to the climate change occurred in the last decades, as a fundamental tool for an adaptive forest management