Supplementary data for article: Šovrc, Ľ.; Borovska, K.; Cinková, K.; Stanković, D.; Planková, A. Advanced Electrochemical Platform for Determination of Cytostatic Drug Flutamide in Various Matrices Using a Boron-Doped Diamond Electrode. Electrochimica Acta 2017, 251, 621–630. https://doi.org/10.1016/j.electacta.2017.08.077

Supplementary material for: [https://doi.org/10.1016/j.electacta.2017.08.077]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2540]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2996

Supplementary data for article: Šovrc, Ľ.; Borovska, K.; Cinková, K.; Stanković, D.; Planková, A. Advanced Electrochemical Platform for Determination of Cytostatic Drug Flutamide in Various Matrices Using a Boron-Doped Diamond Electrode. Electrochimica Acta 2017, 251, 621–630. https://doi.org/10.1016/j.electacta.2017.08.077

Supplementary material for: [https://doi.org/10.1016/j.electacta.2017.08.077]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2540]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2996

Characterization of Essential Oil Composition in Different Basil Species and Pot Cultures by a GC-MS Method

Basil (Ocimum L.) species are used as medicinal plants due to their essential oils exhibiting specific biological activity. The present work demonstrated that both the variety and season/conditions of cultivation had a significant effect on (i) the produced amount (extraction yield), (ii) qualitative, as well as (iii) quantitative profile of basil essential oil. Among studied basil varieties, a new variety, ‘Mánes’, was characterized for the first time. Based on our quantitative evaluation of GC-MS profiles, the following chemotypes and average concentrations of a main component were detected in the studied basil varieties: ‘Ohře’, ‘Lettuce Leaf’, ‘Purple Opaal’, ‘Dark Green’ (linalool, 5.99, 2.49, 2.34, 2.01 mg/mL, respectively), and ‘Mammolo Genovese’, ‘Mánes’, ‘Red Rubin’ (eucalyptol, 1.34, 0.96, 0.76 mg/mL, respectively). At the same time, when considering other compounds identified in GC-MS profiles, all the studied varieties, except from ‘Lettuce Leaf’, were methyl eugenol-rich with a strong dependence of the eugenol:methyl eugenol ratio on the seasonal changes (mainly solar irradiation, but also temperature and relative humidity). More complex and/or variable (depending on the season and cultivation) chemotypes were observed with ‘Lettuce Leaf’ (plus estragole, 2.27 mg/mL), ‘Dark Green’ (plus eucalyptol, 1.36 mg/mL), ‘Mammolo Genovese’ (plus eugenol, 1.19 mg/mL), ‘Red Rubin’ (plus linalool and eugenol, 0.46 and 0.56 mg/mL, respectively), and ‘Mánes’ (plus linalool and eugenol, 0.58 and 0.40 mg/mL, respectively). When considering superior extraction yield (ca. 17 mL·kg−1, i.e., two to five times higher than other examined varieties) and consistent amounts (yields) of essential oil when comparing inter-seasonal or inter-year data (RSD and inter-year difference in mean yield values ˂2.5%), this new basil variety is very promising for use in the pharmaceutical, food, and cosmetic industries

Voltammetric protocol for reliable determination of a platelet aggregation inhibitor dipyridamole on a bare miniaturized boron-doped diamond electrochemical sensor

In present paper, a miniaturized boron-doped diamond electrode (BDDE) was for the first time used as a reliable electrochemical sensor within the novel voltammetric protocol for the rapid and simple determination of dipyridamole (DIP). Cyclic voltammetric studies uncovered that the electrochemical oxidation of DIP was shown to be irreversible and diffusion-controlled process with single and well-developed signal at a potential of +0.45 V in Britton-Robinson buffer at pH 3.0. Using the suitable experimental conditions, the linearity for DIP determination was observed in the concentration ranges of 0.1.5.0 μM and 0.1.6.0 μM with the corresponding limits of detection of 40 nM and 60 nM as well as the intra-day repeatability with relative standard deviation below 5% for differential pulse and square-wave voltammetry, respectively. This protocol was successfully applied to the determination of DIP in pharmaceutical tablets with result in good compliance with that declared by the producer. Biological pertinence of the developed procedure was presented by analysis of model human urine samples with adequate recoveries. The proposed protocol with a miniaturized BDDE could represent an effective and alternative analytical implement for DIP determination instead of commonly used toxic mercury and chemically-modified electrodes in previously published methods

Advanced electrochemical platform for determination of cytostatic drug flutamide in various matrices using a boron-doped diamond electrode

An innovative, rapid and simple electrochemical approach for the reliable quantification of cytostatic drug flutamide (FLU) in various matrices is herein proposed. This platform involves coupling of differential pulse (DPV) and square-wave voltammetry (SWV) with a boron-doped diamond (BDD) electrode as the working electrode and 0.1 M sulphuric acid as the supporting electrolyte. For the first time, the voltammetric profile of FLU was manifested by three irreversible and diffusion-controlled oxidation peaks at + 1.1 (P1), + 1.4 (P2) and + 1.9 V (P3). The analytical performance evaluation was assessed for all three peaks, using both pulse voltammetric techniques with the optimized operating parameters and the highest sensitivity of 1.76 nA/mu M was accomplished for P2 using DPV and 3.54 nA/mu M for P3 using SWV. The corresponding linear concentration ranges were found to be 0.99-42.9 and 4.8-35.5 mu M with the detection limits of 0.42 and 0.18 mu M, respectively. The repeatability varied, depending on the oxidation peaks of FLU, with the relative standard deviations in the range of 3.3-8.8% and 2.9-9.3% for DPV and SWV, respectively. The proposed electrochemical platform was successfully applied in the analysis of pharmaceutical formulations, spiked human urine and water samples with the significant mean recoveries. Using BDD electrode, the current work establishes an advanced, simple and rapid alternative platform to so far used toxic mercury-based electrodes and time demanding chemically modified electrodes in cytostatic sensing. Besides, BDD electrode represents a comfortable electrochemical sensor for routine analysis in pharmaceutical, clinical and environmental chemistry. (C) 2017 Elsevier Ltd. All rights reserved.11th International Symposium on Electrochemical Micro and Nanosystem Technologies (EMNT), Aug 17-19, 2016, Royal Belgian Inst Nat Sci, Brussels, Belgiu