28 research outputs found
Generation of high-value products by photosynthetic microorganisms: from sunlight to biofuels
Algae-Bacteria Consortia as a Strategy to Enhance H2 Production
Biological hydrogen production by microalgae is a potential sustainable, renewable and clean source of energy. However, many barriers limiting photohydrogen production in these microorganisms remain unsolved. In order to explore this potential and make biohydrogen industrially affordable, the unicellular microalga Chlamydomonas reinhardtii is used as a model system to solve barriers and identify new approaches that can improve hydrogen production. Recently, Chlamydomonasâbacteria consortia have opened a new window to improve biohydrogen production. In this study, we review the different consortia that have been successfully employed and analyze the factors that could be behind the improved H2 production
Stenotrophomonas goyi sp. nov., a novel bacterium associated with the alga Chlamydomonas reinhardtii [version 2; peer review: 2 approved]
Background: A culture of the green algae Chlamydomonas reinhardtii was accidentally contaminated with three different bacteria in our laboratory facilities. This contaminated alga culture showed increased algal biohydrogen production. These three bacteria were independently isolated. Methods: The chromosomic DNA of one of the isolated bacteria was extracted and sequenced using PacBio technology. Tentative genome annotation (RAST server) and phylogenetic trees analysis (TYGS server) were conducted. Diverse growth tests were assayed for the bacterium and for the alga-bacterium consortium. Results: Phylogenetic analysis indicates that the bacterium is a novel member of the Stenotrophomonas genus that has been termed in this work as S. goyi sp. nov. A fully sequenced genome (4,487,389 base pairs) and its tentative annotation (4,147 genes) are provided. The genome information suggests that S. goyi sp. nov. is unable to use sulfate and nitrate as sulfur and nitrogen sources, respectively. Growth tests have confirmed the dependence on the sulfur-containing amino acids methionine and cysteine. S. goyi sp. nov. and Chlamydomonas reinhardtii can establish a mutualistic relationship when cocultured together. Conclusions: S. goyi sp. nov. could be of interest for the design of biotechnological approaches based on the use of artificial microalgae-bacteria multispecies consortia that take advantage of the complementary metabolic capacities of their different microorganisms
A mutant in the ADH1 gene of Chlamydomonas reinhardtii elicits metabolic restructuring during anaerobiosis
The green alga Chlamydomonas reinhardtii has numerous genes encoding enzymes that function in fermentative pathways. Among these, the bifunctional alcohol/acetaldehyde dehydrogenase (ADH1), highly homologous to the Escherichia coli AdhE enzyme, is proposed to be a key component of fermentative metabolism. To investigate the physiological role of ADH1 in dark anoxic metabolism, a Chlamydomonas adh1 mutant was generated. We detected no ethanol synthesis in this mutant when it was placed under anoxia; the two other ADH homologs encoded on the Chlamydomonas genome do not appear to participate in ethanol production under our experimental conditions. Pyruvate formate lyase, acetate kinase, and hydrogenase protein levels were similar in wild-type cells and the adh1 mutant, while the mutant had significantly more pyruvate:ferredoxin oxidoreductase. Furthermore, a marked change in metabolite levels (in addition to ethanol) synthesized by the mutant under anoxic conditions was observed; formate levels were reduced, acetate levels were elevated, and the production of CO(2) was significantly reduced, but fermentative H(2) production was unchanged relative to wild-type cells. Of particular interest is the finding that the mutant accumulates high levels of extracellular glycerol, which requires NADH as a substrate for its synthesis. Lactate production is also increased slightly in the mutant relative to the control strain. These findings demonstrate a restructuring of fermentative metabolism in the adh1 mutant in a way that sustains the recycling (oxidation) of NADH and the survival of the mutant (similar to wild-type cell survival) during dark anoxic growth
Low oxygen levels contribute to improve photohydrogen production in mixotrophic nonâstressed Chlamydomonas cultures
Background: Currently, hydrogen fuel is derived mainly from fossil fuels, but there is an increasing interest in clean
and sustainable technologies for hydrogen production. In this context, the ability of some photosynthetic microorganisms,
particularly cyanobacteria and microalgae, to produce hydrogen is a promising alternative for renewable,
clean-energy production. Among a diverse array of photosynthetic microorganisms able to produce hydrogen, the
green algae Chlamydomonas reinhardtii is the model organism widely used to study hydrogen production. Despite
the well-known fact that acetate-containing medium enhances hydrogen production in this algae, little is known
about the precise role of acetate during this process.
Results: We have examined several physiological aspects related to acetate assimilation in the context of hydrogen
production metabolism. Measurements of oxygen and CO2 levels, acetate uptake, and cell growth were performed
under different light conditions, and oxygenic regimes. We show that oxygen and light intensity levels control acetate
assimilation and modulate hydrogen production. We also demonstrate that the determination of the contribution of
the PSII-dependent hydrogen production pathway in mixotrophic cultures, using the photosynthetic inhibitor DCMU,
can lead to dissimilar results when used under various oxygenic regimes. The level of inhibition of DCMU in hydrogen
production under low light seems to be linked to the acetate uptake rates. Moreover, we highlight the importance of
releasing the hydrogen partial pressure to avoid an inherent inhibitory factor on the hydrogen production.
Conclusion: Low levels of oxygen allow for low acetate uptake rates, and paradoxically, lead to efficient and sustained
production of hydrogen. Our data suggest that acetate plays an important role in the hydrogen production
process, during non-stressed conditions, other than establishing anaerobiosis, and independent of starch accumulation.
Potential metabolic pathways involved in hydrogen production in mixotrophic cultures are discussed. Mixotrophic
nutrient-replete cultures under low light are shown to be an alternative for the simultaneous production of
hydrogen and biomass
Chaperones involved in the assembly and export of Tat-dependent proteins
EThOS - Electronic Theses Online ServiceGBUnited Kingdo
Assembly of Tat-dependent [NiFe] hydrogenases: identification of precursor-binding accessory proteins
The Escherichia coli twinâarginine translocation (Tat) system serves to export fully folded protein substrates across the bacterial cytoplasmic membrane. Respiratory [NiFe] hydrogenases are synthesised as precursors with twinâarginine signal peptides and transported as large, cofactorâcontaining, multiâsubunit complexes by the Tat system. Cofactor insertion and assembly of [NiFe] hydrogenases requires coordination of networks of accessory proteins. In this work we utilise a bacterial twoâhybrid assay to demonstrate proteinâprotein interactions between the uncharacterised chaperones HyaE and HybE with Tat signal peptideâbearing hydrogenase precursors. It is proposed that the chaperones act at a âproofreadingâ stage in hydrogenase assembly and police the protein transport pathway preventing premature targeting of Tatâdependent hydrogenases