PCR results for the three major thermophilic amoeba species found in Guadeloupe.

<p>PCR detection and agarose gel electrophoresis of ITS rDNA of <i>N. fowleri</i> (A), <i>N. lovaniensis</i> (B) and <i>Hartmannella</i> sp<b>.</b> (C). <b>M :</b> 100 bp ladder, <b>PC</b> : Positive control (DNA of <i>Naegleria fowleri</i> (#. 359)) gives a single PCR product of 359 bp with the NFITS primers and a 448 bp with the ITS primer set. <b><i>N.f</i></b> : <i>Naegleria fowleri, </i><b><i>N.l</i></b> : <i>Naegleria lovaniensis</i>, the ITS amplification gives a 403 bp PCR product while no band was obtained with the NFITS primers, <b><i>Hart</i></b><i>.</i> : <i>Hartmannella</i> sp., the ITS amplification gives a 900 bp while no band was observed with the NFITS primer set.</p

Distribution of sequenced isolates from the different geothermal baths of Guadeloupe.

<p>Distribution of sequenced isolates from the different geothermal baths of Guadeloupe.</p

Number of <i>Naegleria fowleri</i> isolated from the most frequented baths of Guadeloupe according to temperature and pH (2011–12).

<p>ND : non determined.</p

Number of <i>Naegleria fowleri</i> isolated from the most frequented baths of Guadeloupe according to physicochemical characteristics (2011–12).

<p>Number of <i>Naegleria fowleri</i> isolated from the most frequented baths of Guadeloupe according to physicochemical characteristics (2011–12).</p

Survey of free-living amoeba isolated from geothermal recreational waters of Guadeloupe on a montly basis (2012).

<p><b>Sp.:</b> thermophilic amoebae non-Naegleria sp., <b>N.l</b> : Naegleria lovaniensis., <b>N.f</b> : Naegleria fowleri,</p><p>(n) number of amoebae per liter of water; (+ ou -) presence or absence in sediment or swab; (blank) not investigated.</p

Survey of free-living amoeba isolated from geothermal recreational waters of Guadeloupe on a montly basis (2011).

<p><b>Sp. :</b> thermophilic amoebae non-Naegleria sp., <b>N.l</b> : Naegleria lovaniensis., <b>N.f</b> : Naegleria fowleri,</p><p>(n) number of amoebae per liter of water; (+ ou -) presence or absence in sediment or swab; (blank) not investigated.</p

ITS1 sequences of the three isolates identified in the different geothermal waters of Guadeloupe.

<p>ITS1 sequences of the three isolates identified in the different geothermal waters of Guadeloupe.</p