<p>PCR detection and agarose gel electrophoresis of ITS rDNA of <i>N. fowleri</i> (A), <i>N. lovaniensis</i> (B) and <i>Hartmannella</i> sp<b>.</b> (C). <b>M :</b> 100 bp ladder, <b>PC</b> : Positive control (DNA of <i>Naegleria fowleri</i> (#. 359)) gives a single PCR product of 359 bp with the NFITS primers and a 448 bp with the ITS primer set. <b><i>N.f</i></b> : <i>Naegleria fowleri, </i><b><i>N.l</i></b> : <i>Naegleria lovaniensis</i>, the ITS amplification gives a 403 bp PCR product while no band was obtained with the NFITS primers, <b><i>Hart</i></b><i>.</i> : <i>Hartmannella</i> sp., the ITS amplification gives a 900 bp while no band was observed with the NFITS primer set.</p
