3,244 research outputs found
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Chemical underpinning of the tea bag index: An examination of the decomposition of tea leaves
Decomposition is a key flux of terrestrial carbon to the atmosphere. Therefore, gaining a better understanding of how plant litter decomposes in soil, and what governs this process, is vital for global climate models. The Tea Bag Index (TBI) was introduced by Keuskamp et al. (2013) as a novel method for measuring litter decomposition rate and stabilisation. The TBI uses two types of tea bags representing fast (green tea) and slow (rooibos tea) decomposition substrates as standardised litter bags. To date, the TBI method has been used in over 2000 locations across the globe. However, before now, there has been no information on how the composition of the tea leaves change during incubation. These data are crucial in determining the validity of the use of the TBI method globally, to ensure the tea leaves decompose in a way that is representative of so-called “native” litters. To provide chemical underpinning of the TBI method, a laboratory incubation of the tea bags was conducted with destructive sampling at 0, 7, 14, 21, 28, 35, 42, 49, 56, 63, 70, 77, 84, and 91 d. The incubated tea was analysed for total C and N. In addition, C was characterised as alkyl, O-alkyl, aromatic, or carbonyl C using solid-state 13C nuclear magnetic resonance spectroscopy with cross-polarization and magic angle spinning (CPMAS NMR). The results suggest that changes in carbon in both tea types are comparable to other litter studies, with a net decrease in total C and relative proportion of O-alkyl C fraction, which contains carbohydrates and cellulose. We conclude that the decomposition of tea leaves in the bags used in the TBI is representative of other litters
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A comparison of physical soil organic matter fractionation methods for amended soils
Selecting a suitable physical fractionation method, to investigate soil organic matter dynamics, from the
plethora that are available is a difficult task. Using five different physical fractionation methods, on soils either nontreated or with a history of amendment
with a range of exogenous organic matter inputs (Irish moss peat; composted horse manure; garden compost) and a resulting range of carbon contents (6.8 to 22.2%), we show that method selection had a significant impact on both the total C recovered and the distribution of the recovered C between unprotected, physically protected, or chemically protected conceptual pools. These between-method differences most likely resulted from the following: (i) variation in the methodological fractions obtained (i.e., distinguishing between aggregate size classes); (ii) their subsequent designation to conceptual pools (e.g., protected versus unprotected); and (iii) the procedures used in sample pretreatment and subsequent aggregate dispersion and fractionation steps. The performance of each method also varied depending on the amendment in question. The findings emphasise the need for an understanding of the nature of the soil samples under investigation, and the stabilisation mechanism of interest, both prior to method selection and when comparing and interpreting findings from literature studies using different fractionation methods
Normal form analysis of bouncing cycles in isotropic rotor stator contact problems
This work considers analysis of sustained bouncing responses of rotating shafts with nonlinear lateral vibrations due to rotor stator contact. The insight that this is an internal resonance phenomena makes this an ideal system to be studied with the method of normal forms, which assumes that a system may be modelled primarily in terms of just its resonant response components. However, the presence of large non smooth nonlinearities due to impact and rub mean that the method must be extended. This is achieved here by incorporating an alternating frequency/time (AFT) step to capture nonlinear forces. Furthermore, the presence of gyroscopic terms leads to the need to handle complex modal variables, and a rotating coordinate frame must be used to obtain periodic responses. The process results in an elegant formulation that can provide reduced order models of a wide variety of rotor systems, with potentially many nonlinear degrees of freedom. The method is demonstrated by comparing against time simulation of two example rotors, demonstrating high precision on a simple model and approximate precision on a larger model
Peak visual gamma frequency is modified across the healthy menstrual cycle
Fluctuations in gonadal hormones over the course of the menstrual cycle are known to cause functional brain changes and are thought to modulate changes in the balance of cortical excitation and inhibition. Animal research has shown this occurs primarily via the major metabolite of progesterone, allopregnanolone, and its action as a positive allosteric modulator of the GABAA receptor. Our study used EEG to record gamma oscillations induced in the visual cortex using stationary and moving gratings. Recordings took place during twenty females’ mid‐luteal phase when progesterone and estradiol are highest, and early follicular phase when progesterone and estradiol are lowest. Significantly higher (∼5 Hz) gamma frequency was recorded during the luteal compared to the follicular phase for both stimuli types. Using dynamic causal modeling, these changes were linked to stronger self‐inhibition of superficial pyramidal cells in the luteal compared to the follicular phase. In addition, the connection from inhibitory interneurons to deep pyramidal cells was found to be stronger in the follicular compared to the luteal phase. These findings show that complex functional changes in synaptic microcircuitry occur across the menstrual cycle and that menstrual cycle phase should be taken into consideration when including female participants in research into gamma‐band oscillations
Observation of bosonic coalescence of photon pairs
Quantum theory predicts that two indistinguishable photons incident on a
beam-splitter interferometer stick together as they exit the device (the pair
emerges randomly from one port or the other). We use a special
photon-number-resolving energy detector for a direct loophole-free observation
of this quantum-interference phenomenon. Simultaneous measurements from two
such detectors, one at each beam-splitter output port, confirm the absence of
cross-coincidences.Comment: 4 pages, 2 figures, submitted to Phys. Rev. Let
Generative modelling of the thalamo-cortical circuit mechanisms underlying the neurophysiological effects of ketamine
Cortical recordings of task-induced oscillations following subanaesthetic ketamine administration demonstrate alterations in amplitude, including increases at high-frequencies (gamma) and reductions at low frequencies (theta, alpha). To investigate the population-level interactions underlying these changes, we implemented a thalamo-cortical model (TCM) capable of recapitulating broadband spectral responses. Compared with an existing cortex-only 4-population model, Bayesian Model Selection preferred the TCM. The model was able to accurately and significantly recapitulate ketamine-induced reductions in alpha amplitude and increases in gamma amplitude. Parameter analysis revealed no change in receptor time-constants but significant increases in select synaptic connectivity with ketamine. Significantly increased connections included both AMPA and NMDA mediated connections from layer 2/3 superficial pyramidal cells to inhibitory interneurons and both GABAA and NMDA mediated within-population gain control of layer 5 pyramidal cells. These results support the use of extended generative models for explaining oscillatory data and provide in silico support for ketamine's ability to alter local coupling mediated by NMDA, AMPA and GABA-A
Normal form analysis of stator rub in rotating machinery
This work considers analysis of sustained impacting cycles of rotating shafts with potentially many disks. The insight that this is an internal resonance phenomena makes this an ideal system to be studied with the method of normal forms. However, the presence of arbitrary non smooth nonlinearities due to impact and rub mean that the method must be extended by incorporating an Alternating Frequency/Time (AFT) step to capture nonlinear forces. The process results in an elegant formulation that can model a very wide variety of rotor systems and is demonstrated by comparing against simulation of a contacting overhung rotor
Harmonic-Balance-Based parameter estimation of nonlinear structures in the presence of Multi-Harmonic response and force
Testing nonlinear structures to characterise their internal nonlinear forces is challenging. Often nonlinear structures are excited by harmonic forces and yield a multi-harmonic response. In many systems, particularly ones with strong nonlinearities, the effect of higher harmonics in the force and responses cannot be ignored. Even if the intended excitation is a single frequency sinusoidal force, the interaction of the shaker and the nonlinear structure can lead to harmonics in the applied force. The effects of these higher harmonics of the input force on nonlinear model identification in structural dynamics are often neglected. The objective of this study is to introduce an identification method, motivated by the alternating frequency/time approach using harmonic balance (AFTHB), which is able to consider both multi-harmonic forces and multi-harmonic responses of the system. The proposed AFTHB method can include all significant harmonics by selecting an appropriate time step and sampling frequency to guarantee the accuracy of the results. An analytical harmonic-balance-based (AHB) approach is also considered for comparison. However, the inclusion of all significant harmonics of the response in the analytical expansion of the nonlinear functions is often cumbersome. Furthermore, the AFTHB method can easily cope with complex nonlinearities such as Coulomb friction and with multi-degree of freedom nonlinear systems. Including the effect of higher harmonics in the identification process reduces the approximation error due to truncation and very accurate approximation of the balanced equations of each harmonic is obtained. The proposed identification method requires prior knowledge or an appropriate estimation of the type of system nonlinearities. However, the method of model selection may be used for a set of candidate models, and avoiding a dictionary of arbitrary candidate basis functions significantly reduces the computational costs. This paper highlights the important features of the AFTHB method to ensure accurate estimation using four simulated and two experimental examples. The effects of the number of harmonics considered, the modelling error, measurement noise and the frequency range on the quality of the estimated model are demonstrated
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Effects of repeated application of organic soil amendments on horticultural soil physicochemical properties, nitrogen budget and yield
Application of organic amendments to soil is commonplace in domestic gardening. However, a vast array of materials could be labelled as ‘compost’ by retailers and suppliers. We investigated six different amendments currently used, or available for use, in horticulture: composted bark,
composted bracken, spent mushroom compost, composted horse manure, garden waste compost (at two different application rates), and peat. Using a controlled field experiment, we examined the physicochemical differences between the amendments, the subsequent effects on soil characteristics,
and resultant yield and biometrics of Lavatera trimiestris. Amended soils resulted in a significantly different multivariate soil environment and N budget when compared to the unamended control. However, the effect on yield and plant biometrics (number of flowers, plant height, etc.) depended on the amendment used. Application of garden compost resulted in up to a five-fold increase in yield.
However, there was no significant difference in yields in soils amended with composted bark or peat, when compared to the unamended control. This has implications, as there is increasing pressure to remove peat from products available to domestic gardeners. The variability in the different amendments investigated in our research, in addition to the variable effects on plant growth parameters,
suggests that repeated use of a single amendment may not be best practise for gardeners
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