What's more general than a whole population?

Statistical inference is commonly said to be inapplicable to complete population studies, such as censuses, due to the absence of sampling variability. Nevertheless, in recent years, studies of whole populations, e.g., all cases of a certain cancer in a given country, have become more common, and often report p values and confidence intervals regardless of such concerns. With reference to the social science literature, the current paper explores the circumstances under which statistical inference can be meaningful for such studies. It concludes that its use implicitly requires a target population which is wider than the whole population studied - for example future cases, or a supranational geographic region - and that the validity of such statistical analysis depends on the generalizability of the whole to the target population

Fluorescent turn-on probes for wash-free mRNA imaging via covalent site-specific enzymatic labeling.

Investigating the many roles RNA plays in cellular regulation and function has increased demand for tools to explore RNA tracking and localization within cells. Our recently reported RNA-TAG (transglycosylation at guanine) approach uses an RNA-modifying enzyme, tRNA-guanine transglycosylase (TGT), to accomplish covalent labeling of an RNA of interest with fluorescent tracking agents in a highly selective and efficient manner. Unfortunately, labeling by this method currently suffers from a high nonspecific fluorescent background and is currently unsuitable for imaging RNA within complex cellular environments. Herein we report the design and synthesis of novel fluorogenic thiazole orange probes that significantly lower nonspecific binding and background fluorescence and, as a result, provide up to a 100-fold fluorescence intensity increase after labeling. Using these fluorogenic labeling agents, we were able to image mRNA expressed in Chinese Hamster Ovary cells in a wash-free manner

The Propoganda of Endurance: Identity, Survival, and British Trench Newspapers in the First World War

This study explores the newspapers produced by British officers and men on the Western Front during the First World War. Although subject to censorship, significant scope was granted to the writers and editors of trench journals to express a seemingly strange combination of piety, humor, anger, and sadness concerning the course of the war. Trench newspapers therefore functioned as a cultural space in which the privations and competing desires of military life could be mediated. Through the juxtaposition of varying tones and views of the war, trench newspapers ultimately served to reinforce the hegemonic culture and values of the British Army by functioning as a propaganda of identity and endurance on the Western Front. British trench newspapers both implicitly and explicitly compare the identities and experiences of soldiers and civilians, men and women, and officer and other ranks as British soldier-writers perceived them. In this way, British trench newspapers were able to examine the conflicts and privations of military life while ultimately reinforcing a common identity for British soldiers. Although civilians could be depicted as foolish or myopic concerning the course of the war, the trench journals could also express gratitude for gifts sent from Britain. Soldier-writers depict women much more favorably, though often simplistically as symbols of home or as objects of sexual desire. Within censorship, the trench newspapers express considerable skepticism, through humor, of the General Staff and the course of the war, but the necessity of fighting the war is never explicitly questioned. Finally, by addressing both violence and technology, trench newspapers obscure the agency of individual British soldiers in killing the enemy and dream of a swift conclusion to the war. Trench newspapers, therefore, are an invaluable resource for understanding the cultural history of the First World War

On shocks driven by high-mass planets in radiatively inefficient disks. I. Two-dimensional global disk simulations

Recent observations of gaps and non-axisymmetric features in the dust distributions of transition disks have been interpreted as evidence of embedded massive protoplanets. However, comparing the predictions of planet-disk interaction models to the observed features has shown far from perfect agreement. This may be due to the strong approximations used for the predictions. For example, spiral arm fitting typically uses results that are based on low-mass planets in an isothermal gas. In this work, we describe two-dimensional, global, hydrodynamical simulations of disks with embedded protoplanets, with and without the assumption of local isothermality, for a range of planet-to-star mass ratios 1-10 M_jup for a 1 M_sun star. We use the Pencil Code in polar coordinates for our models. We find that the inner and outer spiral wakes of massive protoplanets (M>5 M_jup) produce significant shock heating that can trigger buoyant instabilities. These drive sustained turbulence throughout the disk when they occur. The strength of this effect depends strongly on the mass of the planet and the thermal relaxation timescale; for a 10 M_jup planet embedded in a thin, purely adiabatic disk, the spirals, gaps, and vortices typically associated with planet-disk interactions are disrupted. We find that the effect is only weakly dependent on the initial radial temperature profile. The spirals that form in disks heated by the effects we have described may fit the spiral structures observed in transition disks better than the spirals predicted by linear isothermal theory.Comment: 10 pages, 8 figures. ApJ, accepte