Best practices for managing large CryoEM facilities

This paper provides an overview of the discussion and presentations from the Workshop on the Management of Large CryoEM Facilities held at the New York Structural Biology Center, New York, NY on February 6–7, 2017. A major objective of the workshop was to discuss best practices for managing cryoEM facilities. The discussions were largely focused on supporting single-particle methods for cryoEM and topics included: user access, assessing projects, workflow, sample handling, microscopy, data management and processing, and user training

Photomatrix computer interface : on line registration of optical configurations for visual pattern recognition

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Minimising Total Costs of a Two-Echelon Multi-Depot Capacitated Vehicle Routing Problem (2E-MD-CVRP) that Describes the Utilisation of the Amsterdam City Canal Network for Last Mile Parcel Delivery

Part 13: Smart Methods and Techniques for Sustainable Supply Chain ManagementInternational audienceAn increase in e-shopping and (last mile) parcel deliveries has contributed to a rapid growth of urban freight transportation. This generates major impacts on city sustainability and liveability. Current solutions for urban logistics concern road traffic, but multiple Dutch cities have an extensive range of city canals that could be used for freight transportation over water. It was investigated how the city canal network of Amsterdam can be utilised for last mile parcel delivery, and what the related effects are. A MILP formulation of a Two-Echelon, Multi-Depot, Capacitated Vehicle Routing Problem (2E-MD-CVRP) was developed. The model describes a network in which ships transport parcels to pre-determined satellite locations in the city centre, where the parcels are transferred to cargo e-bikes for the last mile of the delivery to the customer. The model was optimised by minimising the total costs, using the Genetic Algorithm (GA). The algorithm was able to find solutions but could not always stay within the constrained search space. Different possible network scenarios were evaluated, describing the consequences with respect to emissions, costs, and traffic flows. The results show promising economic, social, and environmental outcomes for a network with ships and cargo e-bikes instead of delivery vans. A daily and investment cost reduction of 16% and 36% respectively and a $$CO_2$$CO2 emission reduction of 78.26% can be realised

Computing the Greedy Spanner in Linear Space

The greedy spanner is a high-quality spanner: its total weight, edge count and maximal degree are asymptotically optimal and in practice significantly better than for any other spanner with reasonable construction time. Unfortunately, all known algorithms that compute the greedy spanner of n points use Omega(n^2) space, which is impractical on large instances. To the best of our knowledge, the largest instance for which the greedy spanner was computed so far has about 13,000 vertices. We present a O(n)-space algorithm that computes the same spanner for points in R^d running in O(n^2 log^2 n) time for any fixed stretch factor and dimension. We discuss and evaluate a number of optimizations to its running time, which allowed us to compute the greedy spanner on a graph with a million vertices. To our knowledge, this is also the first algorithm for the greedy spanner with a near-quadratic running time guarantee that has actually been implemented

Minimising Total Costs of a Two-Echelon Multi-Depot Capacitated Vehicle Routing Problem (2E-MD-CVRP) that Describes the Utilisation of the Amsterdam City Canal Network for Last Mile Parcel Delivery

An increase in e-shopping and (last mile) parcel deliveries has contributed to a rapid growth of urban freight transportation. This generates major impacts on city sustainability and liveability. Current solutions for urban logistics concern road traffic, but multiple Dutch cities have an extensive range of city canals that could be used for freight transportation over water. It was investigated how the city canal network of Amsterdam can be utilised for last mile parcel delivery, and what the related effects are. A MILP formulation of a Two-Echelon, Multi-Depot, Capacitated Vehicle Routing Problem (2E-MD-CVRP) was developed. The model describes a network in which ships transport parcels to pre-determined satellite locations in the city centre, where the parcels are transferred to cargo e-bikes for the last mile of the delivery to the customer. The model was optimised by minimising the total costs, using the Genetic Algorithm (GA). The algorithm was able to find solutions but could not always stay within the constrained search space. Different possible network scenarios were evaluated, describing the consequences with respect to emissions, costs, and traffic flows. The results show promising economic, social, and environmental outcomes for a network with ships and cargo e-bikes instead of delivery vans. A daily and investment cost reduction of 16% and 36% respectively and a 𝐶𝑂2 emission reduction of 78.26% can be realised

Diet of the Blacktip Shark (Carcharhinus limbatus) in the Northwestern Gulf of Mexico

The stomach contents of 90 Blacktip Shark (Carcharhinus limbatus) specimens caught in the northwestern Gulf of Mexico (GOM) were examined. Stomach contents were identified to the lowest possible taxon, and quantified using percent weight, percent number, percent frequency of occurrence, and percent index of relative importance (IRI). Teleosts were the dominant prey group (98.95% IRI), although most were unidentified (61.70% IRI). Of identified teleost species, Atlantic Croaker (Micropogonias undulatus) (28.43% IRI), and Gulf Menhaden (Brevoortia patronus) (2.31% IRI) were the most important. Crustaceans (0.65% IRI), mollusks (0.46% IRI), and elasmobranchs (0.03% IRI) formed a minor component of the diet. Suspected shrimp fishery discards were found in 11% of stomachs, highlighting the potential importance of this food source for the Blacktip Shark. Diet composition did not differ between male and female sharks, but did between juveniles and adults. Juvenile shark diets had greater proportions of unidentified teleost, Clupeidae and Penaeidae, while adult diets had greater proportions of Sciaenidae, Ariidae and cephalopods. Our results were similar, although not identical to, other studies of Blacktip Shark diets in the northwestern GOM. Of note is finding of the mantis shrimp Squilla empusa, a species previously unreported in Blacktip Shark stomach contents. This new finding, the high importance of unidentified teleosts, and the lack of asymptote in the prey accumulation curve emphasize the need for further study of the Blacktip Shark diet in the northwestern GOM