Life-cycle carbon emissions and energy return on investment for 80% domestic renewable electricity with battery storage in California (U.S.A.)

This paper presents a detailed life-cycle assessment of the greenhouse gas emissions, cumulative demand for total and non-renewable primary energy, and energy return on investment (EROI) for the domestic electricity grid mix in the U.S. state of California, using hourly historical data for 2018, and future projections of increased solar photovoltaic (PV) installed capacity with lithium-ion battery energy storage, so as to achieve 80% net renewable electricity generation in 2030, while ensuring the hourly matching of the supply and demand profiles at all times. Specifically—in line with California’s plans that aim to increase the renewable energy share into the electric grid—in this study, PV installed capacity is assumed to reach 43.7 GW in 2030, resulting of 52% of the 2030 domestic electricity generation. In the modelled 2030 scenario, single-cycle gas turbines and nuclear plants are completely phased out, while combined-cycle gas turbine output is reduced by 30% compared to 2018. Results indicate that 25% of renewable electricity ends up being routed into storage, while 2.8% is curtailed. Results also show that such energy transition strategy would be effective at curbing California’s domestic electricity grid mix carbon emissions by 50%, and reducing demand for non-renewable primary energy by 66%, while also achieving a 10% increase in overall EROI (in terms of electricity output per unit of investment)

Role of glycosphingolipid SSEA-3 and FGF2 in the stemness and lineage commitment of multilineage differentiating stress enduring (MUSE) cells

Multilineage differentiating Stress Enduring (MUSE) cells are endogenous, stress-resistant stem cells, expressing pluripotency master genes and able to differentiate in cells of the three embryonic sheets. Stage-Specific Embryonic Antigen 3 (SSEA-3), a glycosphingolipid (GSL), is the marker for identifying MUSE cells and is used to isolate this population from mesenchymal stromal cells. GSLs modulate signal transduction by interacting with plasma membrane components. The growth factor FGF2, important for MUSE cells biology, may interact with GSLs. Specific cell surface markers represent an invaluable tool for stem cell isolation. Nonetheless their role, if any, in stem cell biology is poorly investigated. Functions of stem cells, however, depend on niche external cues, which reach cells through surface markers. We addressed the role of SSEA-3 in MUSE cell behaviour, trying to define whether SSEA-3 is just a marker or if it plays a functional role in this cell population by determining if it has any relationship with FGF2 activity

Molecular and Physiological Effects of Browning Agents on White Adipocytes from Bone Marrow Mesenchymal Stromal Cells

Two different types of adipose depots can be observed in mammals: white adipose tissue (WAT) and brown adipose tissue (BAT). The primary role of WAT is to deposit surplus energy in the form of triglycerides, along with many metabolic and hormonal activities; as thermogenic tissue, BAT has the distinct characteristic of using energy and glucose consumption as a strategy to maintain the core body temperature. Under specific stimuli—such as exercise, cold exposure, and drug treatment—white adipocytes can utilize their extraordinary flexibility to transdifferentiate into brown-like cells, called beige adipocytes, thereby acquiring new morphological and physiological characteristics. For this reason, the process is identified as the ‘browning of WAT’. We evaluated the ability of some drugs, including GW501516, sildenafil, and rosiglitazone, to induce the browning process of adult white adipocytes obtained from differentiated mesenchymal stromal cells (MSCs). In addition, we broadened our investigation by evaluating the potential browning capacity of IRISIN, a myokine that is stimulated by muscular exercises. Our data indicate that IRISIN was effective in promoting the browning of white adipocytes, which acquire increased expression of UCP1, increased mitochondrial mass, and modification in metabolism, as suggested by an increase of mitochondrial oxygen consumption, primarily in presence of glucose as a nutrient. These promising browning agents represent an appealing focus in the therapeutic approaches to counteracting metabolic diseases and their associated obesity

Life-cycle carbon emissions and energy implications of high penetration of photovoltaics and electric vehicles in California

California has set two ambitious targets aimed at achieving a high level of decarbonization in the coming decades, namely (i) to generate 60% and 100% of its electricity using renewable energy (RE) technologies, respectively, by 2030 and by 2045, and (ii) introducing at least 5 million zero emission vehicles (ZEVs) by 2030, as a first step towards all new vehicles being ZEVs by 2035. In addition, in California, photovoltaics (PVs) coupled with lithium-ion battery (LIB) storage and battery electric vehicles (BEVs) are, respectively, the most promising candidates for new RE installations and new ZEVs, respectively. However, concerns have been voiced about how meeting both targets at the same time could potentially negatively affect the electricity grid’s stability, and hence also its overall energy and carbon performance. This paper addresses those concerns by presenting a thorough life-cycle carbon emission and energy analysis based on an original grid balancing model that uses a combination of historical hourly dispatch and demand data and future projections of hourly demand for BEV charging. Five different scenarios are assessed, and the results unequivocally indicate that a future 80% RE grid mix in California is not only able to cope with the increased demand caused by BEVs, but it can do so with low carbon emissions (<110 g CO2-eq/kWh) and satisfactory net energy returns (EROIPE-eq = 12–16)

Muse stem cells can be isolated from stromal compartment of mouse bone marrow, adipose tissue, and ear connective tissue: A comparative study of their in vitro properties

The cells present in the stromal compartment of many tissues are a heterogeneous population containing stem cells, progenitor cells, fibroblasts, and other stromal cells. A SSEA3(+) cell subpopulation isolated from human stromal compartments showed stem cell properties. These cells, known as multilineage-differentiating stress-enduring (MUSE) cells, are capable of resisting stress and possess an excellent ability to repair DNA damage. We isolated MUSE cells from different mouse stromal compartments, such as those present in bone marrow, subcutaneous white adipose tissue, and ear connective tissue. These cells showed overlapping in vitro biological properties. The mouse MUSE cells were positive for stemness markers such as SOX2, OCT3/4, and NANOG. They also expressed TERT, the catalytic telomerase subunit. The mouse MUSE cells showed spontaneous commitment to differentiation in meso/ecto/endodermal derivatives. The demonstration that mul-tilineage stem cells can be isolated from an animal model, such as the mouse, could offer a valid alternative to the use of other stem cells for disease studies and envisage of cellular therapies

Evaluation of Browning Agents on the White Adipogenesis of Bone Marrow Mesenchymal Stromal Cells: A Contribution to Fighting Obesity

Brown-like adipocytes can be induced in white fat depots by a different environmental or drug stimuli, known as "browning" or "beiging". These brite adipocytes express thermogenin UCP1 protein and show different metabolic advantages, such as the ability to acquire a thermogenic phenotype corresponding to standard brown adipocytes that counteracts obesity. In this research, we evaluated the effects of several browning agents during white adipocyte differentiation of bone marrow-derived mesenchymal stromal cells (MSCs). Our in vitro findings identified two compounds that may warrant further in vivo investigation as possible anti-obesity drugs. We found that rosiglitazone and sildenafil are the most promising drug candidates for a browning treatment of obesity. These drugs are already available on the market for treating diabetes and erectile dysfunction, respectively. Thus, their off-label use may be contemplated, but it must be emphasized that some severe side effects are associated with use of these drugs

Impact of lysosomal storage disorders on biology of mesenchymal stem cells: Evidences from in vitro silencing of glucocerebrosidase (GBA) and alpha-galactosidase A (GLA) enzymes.

Lysosomal storage disorders (LDS) comprise a group of rare multisystemic diseases resulting from inherited gene mutations that impair lysosomal homeostasis. The most common LSDs, Gaucher disease (GD), and Fabry disease (FD) are caused by deficiencies in the lysosomal glucocerebrosidase (GBA) and alpha-galactosidase A (GLA) enzymes, respectively. Given the systemic nature of enzyme deficiency, we hypothesized that the stem cell compartment of GD and FD patients might be also affected. Among stem cells, mesenchymal stem cells (MSCs) are a commonly investigated population given their role in hematopoiesis and the homeostatic maintenance of many organs and tissues. Since the impairment of MSC functions could pose profound consequences on body physiology, we evaluated whether GBA and GLA silencing could affect the biology of MSCs isolated from bone marrow and amniotic fluid. Those cell populations were chosen given the former's key role in organ physiology and the latter's intriguing potential as an alternative stem cell model for human genetic disease. Our results revealed that GBA and GLA deficiencies prompted cell cycle arrest along with the impairment of autophagic flux and an increase of apoptotic and senescent cell percentages. Moreover, an increase in ataxia-telangiectasia-mutated staining 1 hr after oxidative stress induction and a return to basal level at 48 hr, along with persistent gamma-H2AX staining, indicated that MSCs properly activated DNA repair signaling, though some damages remained unrepaired. Our data therefore suggest that MSCs with reduced GBA or GLA activity are prone to apoptosis and senescence due to impaired autophagy and DNA repair capacity

Reply to “Comment on ‘The 21 August 2017 Md 4.0 Casamicciola Earthquake: First Evidence of Coseismic Normal Surface Faulting at the Ischia Volcanic Island’ by Nappi et al. (2018)” by V. De Novellis, S. Carlino, R. Castaldo, A. Tramelli, C. De Luca, N. A. Pino, S. Pepe, V. Convertito, I. Zinno, P. De Martino, M. Bonano, F. Giudicepietro, F. Casu, G. Macedonio, M. Manunta, M. Manzo, G. Solaro, P. Tizzani, G. Zeni, and R. Lanari

In this article, we show why the geological model of the 21 August 2017 earthquake proposed by Nappi et al. (2018) has less uncertainty than the sourcemodel proposed by De Novellis et al. (2018). As a matter of fact, the Nappi et al. (2018) model takes into account all geophysical and geological information collected soon after the earthquake. On the contrary, the model proposed by DeNovellis et al. (2018) is based on a limited database, which does not include (1) the available geological and macroseismic information and (2) the extensive scientific literature concerning the correlation between seismic source and surface faulting, also in volcanic areas similar to Ischia. Nevertheless, we are grateful for the comments from De Novellis et al. (2018) because they give us the opportunity to consider the epistemological landscape in which we should frame the research for the best source model of the 21 August 2017 Casamicciola earthquake.Published316-3211T. Deformazione crostale attivaJCR Journa

The 21 August 2017 Md 4.0 Casamicciola Earthquake: First Evidence of Coseismic Normal Surface Faulting at the Ischia Volcanic Island

On 21 August 2017, a shallow earthquake of Md 4.0 struck the CasamicciolaTerme village in the north of Ischia volcanic island (Italy). It caused two fatalities and heavy damage in a restricted area of a few square kilometers. Casamicciola Terme has been recurrently destroyed in the last centuries by similar volcano-tectonic earthquakes (1762, 1767, 1796, 1828, 1881, and 1883). After the catastrophic 1883 Casamicciola event (2343 casualties), this is the first heavy damaging earthquake at Ischia that provides, for the first time, the opportunity of integrating historical seismicity, macroseismic observations, instrumental information, and detailed mapping of coseismic geological effects. Soon after the 2017 mainshock we surveyed the epicentral area to collect data on the coseismic ground effects, recording more than 100 geological field observations. Mapped effects define a belt which closely follows the trace of the Casamicciola E–W-trending normal fault system, bounding the northern slope of Mt. Epomeo, previously known as a Latest Pleistocene to Holocene normal fault with a slip rate of ∼3:0 cm=yr. We found significant evidence for coseismic surface faulting, testified by a main alignment of ruptures for a 2 km end-to-end length and normal dip-slip displacement of 1–3 cm. The geometry and regularity of the structural pattern, together with constant kinematics of the coseismic ruptures with the north side down, strongly suggest a primary tectonic origin for the mapped ruptures and strongly supports an E–W normal-faulting focal mechanism for the 2017 Casamicciola earthquake.Macroseismic information supports the notion that previous historical events also had a similar style of faulting.Published1323-13343T. Sorgente sismicaJCR Journa