10 research outputs found
Erratum to: Analysis of in vitro ADCC and clinical response to trastuzumab: possible relevance of Fc\u3b3RIIIA/Fc\u3b3RIIA gene polymorphisms and HER-2 expression levels on breast cancer cell lines
BACKGROUND: Trastuzumab is a humanized monoclonal antibody (mAb) currently used for the treatment of breast cancer (BC) patients with HER-2 overexpressing tumor subtype. Previous data reported the involvement of FcÎłRIIIA/IIA gene polymorphisms and/or antibody-dependent cellular cytotoxicity (ADCC) in the therapeutic efficacy of trastuzumab, although results on these issues are still controversial. This study was aimed to evaluate in vitro the functional relationships among FcÎłRIIIA/IIA polymorphisms, ADCC intensity and HER-2 expression on tumor target cells and to correlate them with response to trastuzumab. PATIENTS AND METHODS: Twenty-five patients with HER-2 overexpressing BC, receiving trastuzumab in a neoadjuvant (NEO) or metastatic (MTS) setting, were genotyped for the FcÎłRIIIA 158V>F and FcÎłRIIA 131H>R polymorphisms by a newly developed pyrosequencing assay and by multiplex Tetra-primer-ARMS PCR, respectively. Trastuzumab-mediated ADCC of patientsâ peripheral blood mononuclear cells (PBMCs) was evaluated prior to therapy and measured by (51)Chromium release using as targets three human BC cell lines showing different levels of reactivity with trastuzumab. RESULTS: We found that the FcÎłRIIIA 158F and/or the FcÎłRIIA 131R variants, commonly reported as unfavorable in BC, may actually behave as ADCC favorable genotypes, in both the NEO (P ranging from 0.009 to 0.039 and from 0.007 to 0.047, respectively) and MTS (P ranging from 0.009 to 0.032 and P = 0.034, respectively) patients. The ADCC intensity was affected by different levels of trastuzumab reactivity with BC target cells. In this context, the MCF-7 cell line, showing the lowest reactivity with trastuzumab, resulted the most suitable cell line for evaluating ADCC and response to trastuzumab. Indeed, we found a statistically significant correlation between an increased frequency of patients showing ADCC of MCF-7 and complete response to trastuzumab in the NEO setting (P = 0.006). CONCLUSIONS: Although this study was performed in a limited number of patients, it would indicate a correlation of FcÎłR gene polymorphisms to the ADCC extent in combination with the HER-2 expression levels on tumor target cells in BC patients. However, to confirm our findings further experimental evidences obtained from a larger cohort of BC patients are mandatory. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12967-015-0680-0) contains supplementary material, which is available to authorized users
Climate change and food safety: investigation on toxic fish species in the Tyrrhenian sea
RECHARGE.GREEN PROJECT ACTIVITIES IN PILOT AREAS â TESTING AND IMPLEMENTATION (WP6 lead partner report)
In the recharge.green project the work package (WP) 6 was dedicated to test the instruments and models that were developed during the recharge.green project in
differently structured pilot areas and for different renewable energy (RE) sources. Six pilot areas chose different RE according to the availability of the source:
1 Triglav National Park Slovenia forest biomass, 2 Piemont (PNAM) Italy forest biomass and hydropower, 3 Regione Veneto (Mis and MaĂš valley) Italy forest biomass and hydropower, 4 Bavaria Germany hydropower, 5 Vorarlberg Austria forest biomass, hydropower, solar, wind, 6 MegĂšve, Valloire, Belledonne France hydropower later National Park Vercors.
The report described the work carried out in the pilot areas
T-DM1 EFFICACY AND ACTIVITY IN HER2-POSITIVE METASTATIC BREAST CANCER PATIENTS PROGRESSING AFTER FRONTLINE TAXANE PLUS PERTUZUMAB AND TRASTUZUMAB: AN ITALIAN MULTICENTER OBSERVATIONAL STUDY OF THE GRUPPO ITALIANO MAMMELLA (GIM) STUDY GROUP
Gonadotropin-releasing hormone analogues for the prevention of chemotherapy-induced premature ovarian failure in cancer women: Systematic review and meta-analysis of randomized trials.
The role of temporary ovarian suppression with gonadotropin-releasing hormone analogues (GnRHa) in the prevention of chemotherapy-induced premature ovarian failure (POF) is still controversial. We conducted a systematic review and meta-analysis of randomized trials evaluating the efficacy of GnRHa, given before and during chemotherapy, in the prevention of POF in premenopausal cancer patients.JOURNAL ARTICLESCOPUS: re.jinfo:eu-repo/semantics/publishe
Preliminary Experience Using Oncoplastic Techniques of Reduction Mammaplasty and Intraoperative Radiotherapy: Report of 2 Cases
Gonadotropin-releasing hormone analogues for the prevention of chemotherapy-induced premature ovarian failure in cancer women: Systematic review and meta-analysis of randomized trials.
The role of temporary ovarian suppression with gonadotropin-releasing hormone analogues (GnRHa) in the prevention of chemotherapy-induced premature ovarian failure (POF) is still controversial. We conducted a systematic review and meta-analysis of randomized trials evaluating the efficacy of GnRHa, given before and during chemotherapy, in the prevention of POF in premenopausal cancer patients.JOURNAL ARTICLESCOPUS: re.jinfo:eu-repo/semantics/publishe
Geomorphological analysis of the San Domino Island (Tremiti Islands, Southern Adriatic Sea). Results from the 2019 Geomorphological Field Camp of the MSc in Geological Science and Technology (University of Chieti-Pescara)
The 2019 Geomorphological Field Camp at San Domino Island (Tremiti Islands, Southern Adriatic Sea) is the result of geological and geomorphological ïŹeld work activities carried out by a group of students attending the Geomorphological ïŹeld mapping course of the Masterâs Degree in Geological Science and Technology (University of Chieti-Pescara). The main map (1:5000 scale) was obtained through an integrated approach that incorporates morphometric analysis, geological and geomorphological ïŹeld mapping, and geomorphological proïŹles drawing. Activities were carried out by all students, divided into six working groups of three to four persons each. The ïŹeld camp and ïŹeld work activities made it possible to produce a detailed thematic map, as a scientiïŹc tool to depict the San Domino Island landscape, and to outline some geomorphological issues in terms of possible constraints to landscape evolution, geomorphological processes distribution, and natural hazard assessment