Serum Thymus and Activation-Regulated Chemokine Level Monitoring May Predict Disease Relapse Detected by PET Scan after Reduced-Intensity Allogeneic Stem Cell Transplantation in Patients with Hodgkin Lymphoma

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Different biological and prognostic breast cancer populations identified by FDG-PET in sentinel node-positive patients: Results and clinical implications after eight-years follow-up

Abstract Background Sentinel node (SN) biopsy is the standard method to evaluate axillary node involvement in breast cancer (BC). Positron emission tomography with 2-(fluorine-18)-fluoro-2-deoxy-D-glucose (FDG-PET) provides a non-invasive tool to evaluate regional nodes in BC in a metabolic-dependent, biomolecular-related way. In 1999, we initiated a prospective non-randomized study to compare these two methods and to test the hypothesis that FDG-PET results reflect biomolecular characteristics of the primary tumor, thereby yielding valuable prognostic information. Patients and methods A total of 145 cT1N0 BC patients, aged 24–70 years, underwent FDG-PET and lymphoscintigraphy before surgery. SN biopsy was followed in all cases by complete axillary dissection. Pathologic evaluation in tissue sections for involvement of the SN and other non-SN nodes served as the basis of the comparison between FDG-PET imaging and SN biopsy. Results FDG-PET and SN biopsy sensitivity was 72.6% and 88.7%, respectively, and negative predictive values were 80.5% and 92.2%, respectively. A subgroup of more aggressive tumors (ER-GIII, Her2+) was found mainly in the FDG-PET true-positive (FDG-PET+) patients, whereas LuminalA, Mib1 low-rate BCs were significantly undetected ( p = 0.009) in FDG-PET false-negative (FDG-PET−) patients. Kaplan–Meier survival estimates after a median follow-up of more than 8 years showed significantly worse overall survival for FDG-PET+ patients in node-positive (N+) patients ( p = 0.035) as compared to N+/FDG-PET− patients, which overlapped with survival curves of N− and FDG-PET+ or − patients. Conclusions Our findings suggest that FDG-PET results reflect intrinsic biologic features of primary BC tumors and have prognostic value with respect to nodal metastases. FDG-PET false negative cases appear to identify less aggressive indolent metastases. The possibility to identify a subgroup of N+ BC patients with an outcome comparable with N− BC patients could reduce the surgical and adjuvant therapeutic intervention

Methotrexate Increases Skeletal Muscle GLUT4 Expression and Improves Metabolic Control in Experimental Diabetes

Long-term administration of 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) mimics the effects of endurance exercise by activating AMP kinase and by increasing skeletal muscle expression of GLUT4 glucose transporter. AICAR is an intermediate in the purine de novo synthesis, and its tissue concentrations can be increased, in vivo, by low doses of methotrexate (MTX) through the inhibition of the enzyme AICAR transformylase. We report here the first evidence that, in experimental type 2 diabetes, chronic treatment with low doses of MTX increases skeletal muscle GLUT4 expression and improves metabolic control. MTX (0.5 mg/kg body weight) or vehicle was administered intraperitoneally, once a week for 4 weeks, to genetically diabetic female C57BL/KsJ-m+/+Leptdb mice (db+/db+) and their normoglycemic littermates (db+/+m). In the db+/db+ mice, MTX treatment was associated with a ∼2-fold increase in skeletal muscle GLUT4 protein concentration and a >4-fold increase in GLUT4 mRNA expression (P<0.01, all), as compared to vehicle-treated mice; no significant differences were noted in controls. MTX treatment was also associated with a significant reduction of glucose and insulin serum concentrations in diabetic mice (P<0.001), and glucose levels only (P<0.05) in controls. These data indicate a different route to increase skeletal muscle GLUT4 expression, through the potential inhibition of the enzyme AICAR transformylase

Bottlenose Dolphins and Seabirds Distribution Analysis for the Identification of a Marine Biodiversity Hotspot in Agrigento Waters

The aim of this study is to evaluate the presence of biodiversity hotspots in Agrigento waters (Mediterranean Sea) to define the conservation area for bottlenose dolphins (Tursiops truncatus) and seabirds (Calonectris diomedea, Puffinus yelkouan, and Hydrobates pelagicus), according to European directives. With this purpose, the maximum entropy algorithm (MaxEnt) was applied to the sighting points of the focal species. They co-occur in the study area and have been documented to forage behind trawlers. In this study, a fishing rate was designed and used as an explanatory variable of the species distribution, together with physiographic variables. Data were collected during 68 surveys in the waters off Agrigento province. MaxEnt models showed a strong predictive power, with distance from the coast being the greatest predicting variable, followed by slope, depth, and fishing rate. For all the species considered, the probability of presence increased as the fishing rate grew. Cartographic analysis revealed one area shared by the species, which occupies 529 km2, from the shoreline to 100 m depth. This study increases knowledge on the distribution and habitat preferences of the target species in the Sicilian waters. Evaluating the influence of fisheries is a promising method that needs further testing to apply effective management measures

Adapting American Hop (<i>Humulus lupulus</i> L.) Varieties to Mediterranean Sustainable Agriculture: A Trellis Height Exploration

In recent years, Italy’s craft beer industry has seen remarkable growth, fostering the local production of key ingredients, notably hops. However, a research gap exists in exploring open-field hop productivity in typical Mediterranean climates using low-trellis systems. This study addressed this gap by evaluating the productive performances of “Cascade” and “Chinook” hop varieties on “V” trellis systems at different heights (2.60, 3.60, and 4.60 m above ground) in inner Sicily’s Mediterranean climate and soil conditions. The results highlighted the significant impact of trellis height on various parameters, with Cascade displaying exceptional adaptability to low-trellis farming. Key factors like stem and leaf weight emerged as crucial drivers of cone yield, emphasizing their significance in hop cultivation. The distinct responses of Cascade and Chinook varieties to varying trellis heights underscored the need for tailored approaches, offering valuable insights for optimizing hop cultivation practices in semi-arid climates

Fourth International Conference on the Effects of Noise on Aquatic Life

Within the framework of the EU Life+ project named LIFE09 NAT/IT/000190 ARION, a permanent autonomous real-time passive acoustic monitoring system has been implemented in the Portofino Marine Protected Area (IT) for the improvement of the conservation status of bottlenose dolphin (Tursiops truncatus). The system is able to detect and track in real time the simultaneous presence of dolphins and motor boats in the study area. This information is used to prevent collisions and noise pollution by diffusing warning presence messages to the end users involved, as sailors and tourists. In three years of data collecting the proper functioning of the system has been validated. A large number of dolphin pods has been detected and localized. Furthermore different types of motor boats have been tracked in the study area. The system is also able to measure different marine parameters and to obtain the ambient background noise in order to understand the influence of human activities on the dolphin behavior. This type of research is in progress and the first results are shown

ARION System for coastal dolphin conservation: A tool for real-time dolphin passive acoustic monitoring in the Portofino Marine Protected Area

Within the framework of the EU Life+ project named LIFE09 NAT/IT/000190 ARION, a permanent autonomous real-time passive acoustic monitoring system has been implemented in the Portofino Marine Protected Area (IT) for the improvement of the conservation status of bottlenose dolphin (Tursiops truncatus). The system is able to detect and track in real time the simultaneous presence of dolphins and motor boats in the study area. This information is used to prevent collisions and noise pollution by diffusing warning presence messages to the end users involved, as sailors and tourists. In three years of data collecting the proper functioning of the system has been validated. A large number of dolphin pods has been detected and localized. Furthermore different types of motor boats have been tracked in the study area. The system is also able to measure different marine parameters and to obtain the ambient background noise in order to understand the influence of human activities on the dolphin behavior. This type of research is in progress and the first results are shown

Epicardial fat from echocardiography: a new method for visceral adipose tissue prediction

To validate transthoracic echocardiography as an easy and reliable imaging method for visceral adipose tissue (VAT) prediction. VAT is recognized as an important indicator of high cardiovascular and metabolic risk. Several methods are applied to estimate VAT, with different results. We selected 60 healthy subjects (29 women, 31 men, 49.5 +/- 16.2 years) with a wide range of body mass indexes. Each subject underwent transthoracic echocardiogram and magnetic resonance imaging (MRI) to measure epicardial fat thickness on the right ventricle. Measurements of epicardial adipose tissue thickness were obtained from the same echocardiographic and MRI views and points. MRI was also used to measure VAT cross-sectional areas at the level of L4 to L5. Anthropometric indexes were also measured. Subjects with predominant visceral fat accumulation showed higher epicardial adipose tissue thickness than subjects with predominant peripheral fat distribution: 9.97 +/- 2.88 vs. 4.34 +/- 1.98 (p = 0.005) and 7.19 +/- 2.74 vs. 3.43 +/- 1.64 (p = 0.004) in men and women, respectively. Simple linear regression analysis showed an excellent correlation between epicardial adipose tissue and waist circumference (r = 0.895, p = 0.01) and MRI abdominal VAT (r = 0.864, p = 0.01). Multiple regression analysis showed that epicardial adipose tissue thickness (r(2) = 0.442, p = 0.02) was the strongest independent variable correlated to MRI VAT. Bland test confirmed the good agreement between the two methods. Epicardial adipose tissue showed a strong correlation with anthropometric and imaging measurements of VAT. Hence, transthoracic echocardiography could be an easy and reliable imaging method for VAT prediction