I. Studies on the Origin of Soluble-Rna. Ii. Synthesis of Rna in Escherichia Coli Recovering From Magnesium Starvation

119 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1966.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD

Milano. Progetto di riassetto urbano dell'ex caserma mameli. I tracciati come fatto urbano.

Riscrivere la storia di un luogo urbano, risolverlo sviluppando un sistema progettuale complesso, rende necessario cogliere le caratteristiche stesse del luogo, individuare le possibilità e le potenzialità strutturali che lo spazio offre e svelare rapporti talvolta sottesi. Il progetto ha origine dalle necessità e da esse trae forza: l’architettura ideata deriva direttamente dal confronto tra il luogo, la sua storia e le sue trasformazioni, e gli esempi, le architetture che conosciamo, quelle costruite e quelle soltanto disegnate. La risoluzione della Caserma Mameli, la restituzione del suo spazio alla grande città, l’auspicabile creazione di un nuovo nucleo di riferimento per la zona Milano Nord, porta a riflettere sull’importanza del riutilizzo urbano al fine di ottenere nuove unioni e sinergie all’interno del complesso schema della città del XXI secolo. La città contemporanea, in continua trasformazione, vive un’epoca di profondi cambiamenti: si intende progettare e pensare la città in maniera sostenibile, limitando l’impatto della nuova costruzione, favorendo il riutilizzo, urbano ed umano, sviluppando reti sempre più estese per la mobilità collettiva e cercando di limitare quanto più possibile la mera speculazione

Renal Transplants from Older Deceased Donors: Use of Preimplantation Biopsy and Differential Allocation to Dual or Single Kidney Transplant according to Histological Score Has No Advantages over Allocation to Single Kidney Transplant by Simple Clinical Indication

Background. Grafts from elderly donors (ECD) are increasingly allocated to single (SKT) or dual (DKT) kidney transplantation according to biopsy score. Indications and benefits of either procedure lack universal agreement. Methods. A total of 302 ECD-transplants in period from Jan 1, 2000, to Dec 31, 2015, were allocated to SKT (SKTpre) on clinical grounds alone (before Dec 2010, pre-DKT era, n=170) or according to a clinical-histological protocol (after Dec 2010, DKT era, n=132) to DKT (n=48), SKT biopsy-based protocol (“high-risk”, SKThr, n=51), or SKT clinically based protocol (“low-risk”, SKTlr, n=33). Graft and patient survival were compared between the two periods and between different transplant categories. Results. Graft and overall survival in recipients from ECD in pre-DKT and DKT era did not differ (5-year graft survival 87.7% and 84.2%, resp.); equal survival in the 2 ECD periods was shown in both donor age ranges of 60–69 and >70-years, and in low-risk or high-risk ECD categories. Within the DKT protocol SKThr showed worst graft and overall survival in the 60–69 donor age range; DKT did not result in significantly better outcome than SKT from ECD in either era. One-year posttransplant creatinine clearance in recipients did not differ between any ECD transplant category. At 3 and 5 years after transplantation there were significantly higher total dialysis-free recipient life years from an equal donor number in the pre-DKT era than in the DKT protocol. Conclusions. Use of a biopsy-based protocol to allocate grafts from aged donors to SKT or DKT did not result in better short term graft survival than a clinically based protocol with allocation only to SKT and reduced overall recipient dialysis-free life years in time

DUCTOPLASTY USE IN LDLT: A COMPARISON BETWEEN TWO DIFFERENT INSTITUTIONAL EXPERIENCES

C

Polar layered deposits of Mars as seen by MRO/SHARAD

EGU2007-A-0797

Single-cell transcriptomics captures features of human midbrain development and dopamine neuron diversity in brain organoids

Three-dimensional brain organoids have emerged as a valuable model system for studies of human brain development and pathology. Here we establish a midbrain organoid culture system to study the developmental trajectory from pluripotent stem cells to mature dopamine neurons. Using single cell RNA sequencing, we identify the presence of three molecularly distinct subtypes of human dopamine neurons with high similarity to those in developing and adult human midbrain. However, despite significant advancements in the field, the use of brain organoids can be limited by issues of reproducibility and incomplete maturation which was also observed in this study. We therefore designed bioengineered ventral midbrain organoids supported by recombinant spider-silk microfibers functionalized with full-length human laminin. We show that silk organoids reproduce key molecular aspects of dopamine neurogenesis and reduce inter-organoid variability in terms of cell type composition and dopamine neuron formation