Muslime in Europa zwischen Globalisierung und Lokalisierung. Gesellschaftspolitische und theologische Perspektiven im Anschluss an Enes Karic und Tariq Ramadan

). Culture media was collected from hyperoxic conditions (open bars) or normoxic conditions (filled bars) at 24 hours intervals. Final concentrations were estimated from individual standard curves. Generation of endogenous HOwas monitored in separate experiments at the indicated time-points in LSEC cultures by HO-mediated oxidation of DCFH-DA into DFC during 6 h (b). Values are total fluorescence emitted at 545 nm.<p><b>Copyright information:</b></p><p>Taken from "The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells"</p><p>http://www.comparative-hepatology.com/content/7/1/4</p><p>Comparative Hepatology 2008;7():4-4.</p><p>Published online 5 May 2008</p><p>PMCID:PMC2408922.</p><p></p

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maintained in either high (a) or low (b) oxygen tension. Separately, viability was determined at the indicated time points by MTT colorimetric assay (c). Freshly isolated LSECs cultures were established on 24 well-plates and incubated either at hyperoxia (open bars) or at normoxia (filled bars). The obtained results demonstrate a faster decay of loss of cells in cultures maintained at hyperoxic conditions. Statistical analyses by t-student test: *P < 0.05, **P < 0.001.<p><b>Copyright information:</b></p><p>Taken from "The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells"</p><p>http://www.comparative-hepatology.com/content/7/1/4</p><p>Comparative Hepatology 2008;7():4-4.</p><p>Published online 5 May 2008</p><p>PMCID:PMC2408922.</p><p></p

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at normoxia (d-f). The general morphology of the cultures was monitored by light microscopy at day 1 (a, d), day 3 (b, d) and day 5 (c, f) after isolation. Decline of LSECs cultures may be observed in dishes maintained at atmospheric oxygen levels (a-c) after several days of culture.<p><b>Copyright information:</b></p><p>Taken from "The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells"</p><p>http://www.comparative-hepatology.com/content/7/1/4</p><p>Comparative Hepatology 2008;7():4-4.</p><p>Published online 5 May 2008</p><p>PMCID:PMC2408922.</p><p></p

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Nt time-points. Conditioned media were collected from LSECs cultured at hyperoxia (open bars) or normoxia (filled bars) at 24 hours intervals. Final concentrations were estimated from individual standard curves. Values are means of triplicate measurements. The results are representative data obtained from three independent experiments. Statistical analyses by t-student test: *P < 0.001.<p><b>Copyright information:</b></p><p>Taken from "The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells"</p><p>http://www.comparative-hepatology.com/content/7/1/4</p><p>Comparative Hepatology 2008;7():4-4.</p><p>Published online 5 May 2008</p><p>PMCID:PMC2408922.</p><p></p

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Ncubation of cells at normoxic or hyperoxic conditions. Each column represents separate values of cell-associated (lower part) and degraded (upper part) I-FSA. Total endocytosis is the result of adding cell associated and degraded ligand (full column size), calculated as percentage of total I-FSA added to cultures. Values are means of triplicate measurements. The results are representative data obtained from three independent experiments. Statistical analyses by Student's -test: *P < 0.05, **P < 0.001.<p><b>Copyright information:</b></p><p>Taken from "The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells"</p><p>http://www.comparative-hepatology.com/content/7/1/4</p><p>Comparative Hepatology 2008;7():4-4.</p><p>Published online 5 May 2008</p><p>PMCID:PMC2408922.</p><p></p

The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells-2

Nt time points by SEM. LSECs cultures were maintained at high (a-c) or low (d-f) oxygen levels. Highly fenestrated cells can be observed during early time points of culture. Fenestration is gradually lost over time in both normoxic or hypoxic conditions.<p><b>Copyright information:</b></p><p>Taken from "The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells"</p><p>http://www.comparative-hepatology.com/content/7/1/4</p><p>Comparative Hepatology 2008;7():4-4.</p><p>Published online 5 May 2008</p><p>PMCID:PMC2408922.</p><p></p

The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells-3

E excluded from the analysis. Porosity measurements are expressed as percentage of the total area covered by cells in each coverslip. Black columns: 20% oxygen. White columns: 5% oxygen.<p><b>Copyright information:</b></p><p>Taken from "The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells"</p><p>http://www.comparative-hepatology.com/content/7/1/4</p><p>Comparative Hepatology 2008;7():4-4.</p><p>Published online 5 May 2008</p><p>PMCID:PMC2408922.</p><p></p

Visualization of membrane rafts and fenestrations.

<p>(A–C) 3D-SIM of LSECs stained with Bodipy FL C5 ganglioside GM1, a marker for rafts (green) and Cell-Mask Orange, a cell membrane marker (orange). There is an inverse distribution between liver sieve plates and membrane rafts. Membrane rafts are mostly perinuclear while sieve plates are mostly peripheral. Some sieve plates are identified by an asterix (*) and fenestrations can be resolved within the sieve plates. Rafts are shown with arrows (→). The areas marked in a box (clustered rafts) are further magnified in (D–G). (D–G) Magnification of areas in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046134#pone-0046134-g001" target="_blank">Figure 1(A–B)</a> showing clustered membrane rafts with raised perimeters. (H) TIRFM of LSEC stained with NBD-cholesterol (green), a marker for rafts, and CellMask Orange (orange) showing perinuclear distribution of rafts (arrows). Fenestrations are not resolved within the sieve plates (*) with TIRFM. (I) TIRFM of LSEC stained with Bodipy FL C5 ganglioside GM1, a marker for rafts, and CellMask Orange (orange) confirming mostly perinuclear distribution of rafts. Scale bar 5 µm (A, B, H, I), 1 µm (C–G).</p

Effects of manipulating actin on fenestrations.

<p>(A) SEM of control LSEC showing fenestrations clustered in sieve plates. (B) SEM of LSEC following treatment with cytochalasin D showing an increase in fenestrations. (C) SEM of LSEC following treatment with Triton X-100 and cytochalasin D. Triton X-100 ameliorated the effects of cytochalasin D on fenestrations. (D) Two-photon fluorescence microscopy of LSEC following treatment with cytochalasin D and stained with LAURDAN. Numerous sieve plates are apparent. (scale bar 5 µM).</p

Effects of VEGF on membrane rafts.

<p>(A) Two-photon fluorescence microscopy of LSECs stained with LAURDAN. (B) Two-photon fluorescence microscopy of LSECs stained with LAURDAN following treatment with VEGF, showing increased blue staining consistent with increased non-raft lipid disordered membrane.</p