Sampling system for LUCAS 2015

The sampling design of LUCAS 2015 took into account experience from previous campaigns. While remaining a Two-phase sampling scheme, efforts were focused in reducing the set of excluded points from the second phase sample, which are placed in difficult and inaccessible areas. This action was implemented both through: 1. the improvement of the first phase sample (LUCAS master sample) by updating each point of the grid with the most recent available information (NUTS borders, road network, elevation, Corine Land Cover results (CLC2006)) 2. the fine-tuning of the past rule for eligibility of the point introducing an additional new indicator of accessibility based on CLC; the final criteria combine all the auxiliary information [Elevation, Road distance, Slope, CLC indicator]. However excluded points will remain a likely source of bias which has to be treated separately from the field survey. The corresponding excluded area needs to be covered with a complementary photo-interpretation operation, including an ex-post photo-interpretation also for 2012 and 2009 surveys. Such a photo-interpretation task, although important and linked to the sampling exercise, is not addressed in this document but represents next priority for the future work.JRC.H.4-Monitoring Agricultural Resource

Glutamine synthetase inhibitors in cancer

Glutamine synthetase inhibitors in cance

A new one-pot synthesis of quinoline-2-carboxylates under heterogeneous conditions

Quinoline-2-carboxylates are an important subclass of quinoline derivatives largely present in a variety of biologically active molecules, as well as useful ligands in metal-catalyzed reactions. Herein, we present a new one-pot protocol for synthesizing this class of derivatives starting from beta-nitroacrylates and 2-aminobenzaldehydes. In order to optimize the protocol, we investigated several reaction conditions, obtaining the best results using the 2-tert-butylimino-2-diethylamino- 1,3-dimethylperhydro-1,3,2-diazaphosphorine (BEMP) as solid base, in acetonitrile. Finally, we demonstrated the generality of our approach over several substrates which led to synthesize a plethora of functionalized quinolines-2-carboxylate derivatives in good overall yields

Tumor-associated and immunochemotherapy-dependent long-term alterations of the peripheral blood NK cell compartment in DLBCL patients

Natural Killer (NK) cells are a key component of tumor immunosurveillance and thus play an important role in rituximab-dependent killing of lymphoma cells via an antibody-dependent cellular cytotoxicity (ADCC) mechanism. We evaluated the phenotypic and functional assets of peripheral blood NK cell subsets in 32 newly-diagnosed diffuse large B-cell lymphoma (DLBCL) patients and in 27 healthy controls. We further monitored long-term modifications of patient NK cells for up to 12 months after rituximab-based immunochemotherapy. At diagnosis, patients showed a higher percentage of CD56dim and CD16C NK cells, and a higher frequency of GrzBC cells in CD56dim, CD56bright, and CD16C NK cell subsets than healthy controls. Conversely, DLBCL NK cell killing and interferon g (IFNg) production capability were comparable to those derived from healthy subjects. Notably, NK cells from refractory/relapsed patients exhibited a lower “natural” cytotoxicity. A marked and prolonged therapy-induced reduction of both “natural” and CD16- dependent NK cytotoxic activities was accompanied by the down-modulation of CD16 and NKG2D activating receptors, particularly in the CD56dim subset. However, reduced NK cell killing was not associated with defective lytic granule content or IFNg production capability. This study firstly describes tumor-associated and therapy-induced alterations of the systemic NK cell compartment in DLBCL patients. As these alterations may negatively impact rituximab-based therapy efficacy, our work may provide useful information for improving immunochemotherapeutic strategies

Glutamine Synthetase Desensitizes Differentiated Adipocytes to Proinflammatory Stimuli by Raising Intracellular Glutamine Levels

The role of glutamine synthetase (GS), a key glutamine-producing enzyme, is unclear during adipocyte differentiation. We assess here whether GS expression influences the adipocytic response to a proinflammatory challenge at different differentiation stages. GS is expressed at late stages of differentiation and desensitizes mature cells to bacterial lipopolysaccharide (LPS) by increasing intracellular glutamine levels. LPS-activated mature adipocytes are unable to produce inflammatory mediators; their sensitivity to LPS is rescued in conditions of GS inhibition, in which intracellular glutamine levels decrease. The ability of adipocytes at different differentiation days to respond to LPS negatively correlates to GS expression and intracellular glutamine levels. Modulation of intracellular glutamine levels by GS expression represents an endogenous mechanism by which mature adipocytes control the inflammatory response

PROBLEMS OF ROOFING OF EARLY MINOAN THOLOS TOMBS: THE CASE OF KAMILARI A THOLOS TOMB IN THE WESTERN MESARA PLAIN

Due to their reduced state of preservations, the argument of a stone vault for Minoan tholos tombs has been discussed in the past years with open or not unanimous conclusions.Thanks to a special survey carried out during summer 2009 on Kamilari A tholos tombs and the good state of preservation of many architectural parts, the present article wants to explore under a new perspective the issue of the vault system. After the presentation of the problem of roofing of Minoan tholos tombs through archaeological and ethnographical examples, the second part of the paper deals with the discussion of the false and the true vault systems and the analysis of possible roof profiles and collapse mechanisms of the vault. It will be argued that: 1) the true vault behaviour cannot be excluded for Kamilari tholos A; 2) among the possible causes inducing this structure to collapse there is an earthquake hitting the structure along the South East-North West alignment.Finally, further considerations about the relation between Minoan and Mycenaean tholos tombs are stressed

Epidemiology of hydatidosis in the province of Sassari, Italy

Cystic echinococcosis is endemic in certain parts of the world, including Sardinia, Italy. It was performed a study in the province of Sassari in order to evaluate the incidence of the infection in man and the effects of control programs since 1964 to 2002. Data obtained by surgical records, hospital discharge forms, radiological and pathological files were collected using a case report form. During the years 1964- 2002, 2702 new cases were identified (average annual incidence: 17 per 100,000) and 1981 (73.3%) were submitted to surgical treatment. In 57.3% municipalities no cases were observed during the years 1998- 2002. Males are more affected (56.2%), mostly farmers-shepherdess (68.6 per 100,000) and pensioners (59.6 per 100,000). Control measures led to a significant decline in the incidence rate of hydatidosis during the period 1964-2002, dropping by 27.6 per 100,000. The mean age of surgical patients increased during the years of surveillance, such as the surgical liver/lung ratio as a consequence of a cohort effect. The durability of control programs is the corner stone for obtaining a significant decrease of this infection

Human-Centered Design of a Collaborative Robotic System for the Shoe-Polishing Process

Demand for automated processes in the manufacturing industry is now shifting toward flexible, human-centered systems that combine productivity and high product quality, thus combining the advantages of automated and robotic systems with the high-value-added skills of operators and craftsmen. This trend is even more crucial for small and medium-sized enterprises operating in the “Made in Italy” fashion industry. The paper presents the study, simulation, and preliminary testing of a collaborative robotic system for shoe polishing that can reduce manual labor by limiting it to the finishing stage of the process, where the aesthetic result is fully achieved, with a benefit also in terms of ergonomics for the operator. The influence of process parameters and design solutions are discussed by presenting preliminary test results and providing hints for future developments