Age-Dependent Dynamics of Somatometric Indexes of Boys of Junior Scool.

У статті наведено основні антропометричні характеристики тіла хлопчиків молодшого шкільного віку. Визначено й проаналізовано динаміку їх зміни у віковому аспекті. Вивчено темпи приросту ваги, росту, обсягу стегна та гомілки, довжини й ширини стопи в молодших школярів. Проаналізовано ступені кореляційних зв’язків між досліджуваними показниками в кожному віці. n the atticle the basic anthropometric descriptions of body of boys of junior scool age are shown.The dynamics of their change in an age-dependent aspect is analysed anddetermined. The rates of gaining weight, height, hip volume and shin volume, foot length and width of the junior scoolchildren are studied. The degrees of cross-correlation connections are analyzed between the probed indexes of every age-group

The importance of physical activity in the regulation of anti-viral immunity

The paper reviews the current data on the effect and role of varying intensity physical activity in the prevention of various respiratory virus infections, including influenza virus and SARS-CoV-2 coronavirus. The paper discusses the effect of varying intensity physical activity on antiviral immunity, cellular and cytokine responses to respiratory virus infections, physical activity influence on vaccination effectiveness and the role of regular moderate intensity physical activity in the prevention of viral infection in patients with obesity, overweight, diabetes and other metabolic disorders. The paper analyzes physical activity role in the prevention of SARS-CoV-2 infection, as well as in the conditions of self-isolation and quarantine

Study Of Technical Cellulose As A Matrix-Sorbent To Develop Express Analytic System For Water Safety Control

The study presented by the authors is devoted to the study of the properties and the possibility of using technical cellulose from non-wood plant raw materials as a solid-phase matrix to obtain solid-phase reactive indicator systems by the following methods: synthesis method on the base of a hetarylformazane immobilized on a cellulose matrix and development of analytical systems based on preconcentration of the determined metal ion by a matrix with subsequent its «revealing» by the formazan («revealing» method). The article focuses on determination of optimal combinations of chromogenic organic reagents (hetarylformazanes) and cellulose-based matrices for developing solid-phase reaction-based indicator systems. Adsorption features of formazan reagents onto cellulose matrices was studied. It has been established the relation between the reagent molecule structure, composition of cellulose matrix and analytical properties of the test-systems synthesized to determine metal ions. Different approaches were developed and applied to reveal the visually observable and easily measured effect due to cellulose properties as well as properties of hetarylformazanes fixed on the surface of the matrix. This fact allows to control sensitivity and selectivity of solid-phase reactive indicator systems for water quality assessment. © 2021 Altai State University. All rights reserved

The Use of Computer Technologies in the Process of Physical Education of Student Young People.

В статье представлена разработанная авторами информационно -методическая система “Гармония тела”, раскрываются назначение и возможности модулей компьютерной программы. In the article the developed by authors informative- methodical system is presented “Harmo ny of body”, setting and p ossibilities of computer program units open up

Features of the frequency of occurrence of T-330G <i>IL2</i> gene polymorphism in patients with COVID-19

SARS-CoV-2 infection is the etiopathogenetic factor of the new coronavirus infection. Susceptibility to the virus and, accordingly, the incidence differs in children and adults. On the one hand, this reflects the age-related features of the immune response. On the other hand, it is realized through the production of a number of cytokines, including IL-2, and reflects the genetically determined features of cytokine production. The aim of the study was to analyze the frequency of occurrence of T-330G polymorphic variants of the IL2 gene in patients with a new coronavirus infection. A total of 145 patients were examined, including 31.0% of children (n = 45) and 69.0% of adults (n = 100). The diagnosis of a new coronavirus infection was verified by RT-PCR confirming the presence of the SARS-CoV-2 virus and identifying clinical symptoms of an upper respiratory tract infection. The control group consisted of 50 healthy volunteer donors. Allele-specific PCR with electrophoretic detection in 3% agarose gel (Litech, Russia) was used to analyze the T-330G polymorphism of the IL2 gene. To compare the frequencies of allele combinations, the χ2 test and the odds ratio OR and (95% CI) were used.The dominant genotype in patients with COVID-19 was the heterozygous GT genotype of the T-330G polymorphism of the IL2 gene. In the group of children at risk of developing a new coronavirus infection, the GG genotype of the T-330G polymorphism of the IL2 gene was associated (31.1% in children and 18.0% in the control group, p &lt; 0.05, OR = 2.047). While the homozygous TT genotype of the T-330G polymorphism of the IL2 gene was a protective genotype (its occurrence rate was 26.7% in patients, 54.0% in the control group, p &lt; 0.05, OR = 0.315). In adults, the heterozygous GT genotype of the T-330G polymorphism of the IL2 gene was associated with the risk of developing a new coronavirus infection (in the group of patients – 44.0% versus control – 28.0%, p = 0.028, OR = 2.020). A low risk of developing the disease was associated with the homozygous TT variant of the T-330G polymorphism of the IL2 gene (in the group of patients 37.0% versus control – 54.0%, p = 0.024, OR = 0.500).The T-330G polymorphism of the promoter zone of the IL2 gene differently affects its production. The direction of the immune response and its effectiveness depend on the level of IL-2. Understanding the individual factors that determine the features of the immune response can help in understanding the mechanisms of development of COVID-19-associated diseases and the selection of approaches to personalized methods of their treatment

Experimental Evaluation of Application of Cross-Flow Ultrafiltarion Method for O Antigen Concentrating in Cholera Chemical Bivalent Vaccine Production

Demonstrated is possibility to apply cross-flow ultrafiltration method for O antigen of Vibrio cholerae M-41 Ogawa concentrating from germ-free centrifugate. Technological process of concentrating was optimized. Worked out were the regimes of conservation and cleaning of the ultrafiltration device. The prospects of cross-flow ultrafiltration method introduction in technology of cholera chemical bivalent vaccine production were determined

Experimental Technology for O-Antigens Production of Non-Toxigenic Strains of <I>Vibrio cholerae</I>

Determined are the key bio-kinetic indexes of submerged cultivation of Vibrio cholerae non-toxigenic strains, producers of O-antigens. Evaluated is the technology of O-antigen concentration using tangential ultrafiltration technique. The results suggest the principal possibility of using these strains for biologically safe production of chemical cholera vaccines

Deployment of Ultrafiltration for Concentrating and Purification of Antigens

Represented is domestic and foreign literature review dedicated to usage of ultrafiltration for concentrating and purification of antigens. Discussed are the issues of deployment of various ultrafiltration techniques. It is determined that filtering in the tangential mode by means of modules with flat-frame filtering elements is among the prospective ones. Demonstrated is the impact of such technological specifications as concentration rate, pressure, temperature, and membrane nominal cut-off on molecular mass on the quality of target products, the time elapsed, and preparation losses decrease (increase). Literature data analysis proves to be useful for the selection of the proper procedure for concentrating and purification of protective antigens of bacterial and viral origins. In addition, it allows for taking into account the parameters under discussion when developing specific manufacturing technologies for diagnostic and preventive medical immunobiological preparation production

EVALUATION OF VIBRIO CHOLERAE 569B INABA PROTECTIVE ANTIGENES, DERIVED ON INDUSTRIAL AND DEVELOPED BIOREACTORS AS WELL AS BY IMPROVED TECHNOLOGY

We evaluated immunochemical, physical and biochemical properties of Vibrio cholerae 569B INABA protective antigenes, derived on industrial and own-developed bioreactors as well as by technology of its concentration by tangential ultrafiltration. We detected, protease, twinase and. lysophospholipase in all samples. Also, dotimmunoanalysis showed equal concentration, of cholerogen-anatoxine and. O-antigen in all samples too. Using chromatography and. electrophoresis, we found their properties as similar. Thus, we suppose to be possible using developed bioreactor as well as technology of Vibrio cholerae 569B INABA protective antigens concentration by tangential concentration during a process of synthetic oral cholera vaccine production

Properties of Experimental and Standard Preparations of the Protective O Antigen of <I>Vibrio cholerae</I> M41 Ogawa

Investigated are immunochemical, chemical and biochemical properties of the O-antigen preparations obtained from Vibrio cholerae M41 classical biovar, serotype Ogawa strain using standard manufacturing and improved technologies of their concentrating. The preparations have been concentrated by ultrafiltration in the mode of tangential liquid flow with membranes of various cut-off levels for a particular molar weight. ELISA has revealed equal O-antigen load in all of these preparations. Studies of specific fractures by means of chromatographic and electrophoretic methods have demonstrated their properties to be alike. Chemical composition analysis has also identified coincidence in the load of components under specification. Thus, it has been proved that application of the improved V. cholerae protective antigen concentrating technology for manufacturing cholera chemical bivalent palletized vaccine is justified