Cryopreservation of peripheral blood stem cell : the influence of cell concentration on cellular and hematopoietic recovery

11 p. : il.BACKGROUND: The optimal cryopreservation cell concentration during the peripheral blood stem cell (PBSC) collection is a controversial topic. We evaluated the influence of cryopreservation concentration on the recovery of hematopoietic progenitor cells and the kinetics of hematopoietic recovery of autologous stem cell transplant patients. STUDY DESIGN AND METHODS: In this retrospective study, we compared two different cryopreservation protocols: 1 ¥ 108 cells/mL (Protocol A) and 2 ¥ 108 cells/mL (Protocol B). A total of 419 PBSCs were analyzed with regard to the number of viable cells and colony-forming units–granulocytes-monocytes (CFUGM) progenitors. The hematopoietic recovery of 275 patients who received PBSCs cryopreserved at a dose of 1 ¥ 108 cells/mL (Group A) and 2 ¥ 108 cells/mL (Group B) were compared. RESULTS: There were no significant differences in recovery of viable cells between Protocol A and Protocol B. The median of recovery of CFU-GM progenitors was significantly higher in Protocol B (41.2 vs. 57.3, p < 0.01). The median times to neutrophil recovery ( 500 ¥ 106/L) and platelet (PLT) recovery ( 20 ¥ 109/L) in Groups A and B were 11 days versus 11 days and 12 days versus 12 days, respectively. However, by Kaplan and Meier analyses, Group B recovered neutrophils with a little delay (p = 0.01). No difference was observed with regard to time to PLT recovery. On multivariate analysis, we found that the number of CD34+ cells and CFU-GM progenitors had a significant influence on hematopoietic recovery. CONCLUSION: Cryopreservation of PBSCs at a dose of 2 ¥ 108 cells/mL did not affect the recovery rate of viable cells or the hematopoietic recovery of autologous stem cell transplant patients

The use of indicators in the pre-analytical phase as a laboratory management tool

Introduction:Efficient laboratory services are the basis of modern health systems. Scientific innovations have contributed to substantial improvements in the laboratory environment, but errors still persist. These errors are classified as pre-analytical, analytical and post-analytical, according to the time of occurrence.Objective:To evaluate the frequency of pre-analytical errors in the clinical laboratory service of a military hospital.Methods:A total of 329,582 tests were performed in the clinical laboratory of Hospital Naval Marcílio Dias (HNMD) from August to October 2012, and pre-analytical errors were documented.Results:The most frequent cause of the observed pre-analytical errors was hemolysis (27.54%), followed by samples not received (25.43%) and insufficient sample volume (18.49%). The samples from the Integrated Home Care Service (SIAD) showed the highest frequency of errors (3.38%), followed by those from the inpatient (0.76%) and the outpatient departments (0.21%).Conclusion:Our study demonstrates the importance of managing laboratory pre-analytical quality in order to ensure service excellence