Cryopreservation of peripheral blood stem cell : the influence of cell concentration on cellular and hematopoietic recovery
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Abstract
11 p. : il.BACKGROUND: The optimal cryopreservation cell concentration
during the peripheral blood stem cell (PBSC)
collection is a controversial topic. We evaluated
the influence of cryopreservation concentration on the
recovery of hematopoietic progenitor cells and the
kinetics of hematopoietic recovery of autologous stem
cell transplant patients.
STUDY DESIGN AND METHODS: In this retrospective
study, we compared two different cryopreservation
protocols: 1 ¥ 108 cells/mL (Protocol A) and 2 ¥ 108
cells/mL (Protocol B). A total of 419 PBSCs were analyzed
with regard to the number of viable cells and
colony-forming units–granulocytes-monocytes (CFUGM)
progenitors. The hematopoietic recovery of 275
patients who received PBSCs cryopreserved at a dose
of 1 ¥ 108 cells/mL (Group A) and 2 ¥ 108 cells/mL
(Group B) were compared.
RESULTS: There were no significant differences in
recovery of viable cells between Protocol A and Protocol
B. The median of recovery of CFU-GM progenitors
was significantly higher in Protocol B (41.2 vs. 57.3,
p < 0.01). The median times to neutrophil recovery
( 500 ¥ 106/L) and platelet (PLT) recovery
( 20 ¥ 109/L) in Groups A and B were 11 days versus
11 days and 12 days versus 12 days, respectively.
However, by Kaplan and Meier analyses, Group B
recovered neutrophils with a little delay (p = 0.01). No
difference was observed with regard to time to PLT
recovery. On multivariate analysis, we found that the
number of CD34+ cells and CFU-GM progenitors had a
significant influence on hematopoietic recovery.
CONCLUSION: Cryopreservation of PBSCs at a dose
of 2 ¥ 108 cells/mL did not affect the recovery rate of
viable cells or the hematopoietic recovery of autologous
stem cell transplant patients