Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study

Abstract Background The placenta is an important site for iron metabolism in humans. It transfers iron from the mother to the fetus. One of the major iron transport proteins is transferrin, which is a blood plasma protein crucial for iron uptake. Its localization and expression may be one of the markers to distinguish placental dysfunction. Methods In the experimental study we used antibody preparation, mass spectrometric analysis, biochemical and immunocytochemical methods for characterization of transferrin expression on the human choriocarcinoma cell line JAR (JAR cells), placental lysates, and cryostat sections. Newly designed monoclonal antibody TRO-tf-01 to human transferrin was applied on human placentae from normal (n = 3) and abnormal (n = 9) pregnancies. Results Variations of transferrin expression were detected in villous syncytiotrophoblast, which is in direct contact with maternal blood. In placentae from normal pregnancies, the expression of transferrin in the syncytium was significantly lower (p \u3c 0.001) when compared to placentae from abnormal ones (gestational diabetes, pregnancy induced hypertension, drug abuse). Conclusion These observations suggest that in the case of abnormal pregnancies, the fetus may require higher levels of transferrin in order to prevent iron depletion due to the stress from the placental dysfunction

Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study

Abstract Background The placenta is an important site for iron metabolism in humans. It transfers iron from the mother to the fetus. One of the major iron transport proteins is transferrin, which is a blood plasma protein crucial for iron uptake. Its localization and expression may be one of the markers to distinguish placental dysfunction. Methods In the experimental study we used antibody preparation, mass spectrometric analysis, biochemical and immunocytochemical methods for characterization of transferrin expression on the human choriocarcinoma cell line JAR (JAR cells), placental lysates, and cryostat sections. Newly designed monoclonal antibody TRO-tf-01 to human transferrin was applied on human placentae from normal (n = 3) and abnormal (n = 9) pregnancies. Results Variations of transferrin expression were detected in villous syncytiotrophoblast, which is in direct contact with maternal blood. In placentae from normal pregnancies, the expression of transferrin in the syncytium was significantly lower (p Conclusion These observations suggest that in the case of abnormal pregnancies, the fetus may require higher levels of transferrin in order to prevent iron depletion due to the stress from the placental dysfunction.</p

Faecal bacterial composition in dairy cows shedding Mycobacterium avium subsp. paratuberculosis in faeces in comparison to non-shedding cows

The aim of this study was to determine possible differences in the faecal microbiota of dairy cows infected with Mycobacterium avium subsp. paratuberculosis (Johne’s disease) in comparison with not infeced cows from the same herds. Faecal samples from cows in four herds were tested using real-time PCR for Mycobacterium avium subsp. paratuberculosis and faecal bacterial populations were analysed using 454 pyrosequencing of the 16S rRNA gene. Most notable differences between shedding and not shedding cows included an increase in the genus Psychrobacter and a decrease in Oscillospira, Ruminoccocus and Bifidobacterium genera in cows infected with M. avium subsp. paratuberculosis. The present study is the first to report the faecal microbial composition in dairy cows infected with M. avium subsp. paratuberculosis.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

High Prevalence of Neutrophil Cytoplasmic Autoantibodies in Infants with Food Protein-Induced Proctitis/Proctocolitis: Autoimmunity Involvement?

Background. Food protein-induced proctitis/proctocolitis (FPIP) is the most common noninfectious colitis in children in the first year of life. Along with the overall clinical symptoms, diarrhoea and rectal bleeding are the main manifestations of the disease. There is no routine noninvasive test that would be specific for this type of colitis. The aim of our study was to find a noninvasive laboratory test or tests that may be helpful in differential diagnosis of food protein-induced proctitis/proctocolitis. Methods. ANA, ANCA, ASCA, a-EMA, a-tTg, specific IgE, total IgE, IgG, IgA, IgM, and concentration of serum calprotectin were measured in a group of 25 patients with colitis and 18 children with other diagnoses. Results. Atypical-pANCA antibodies of IgG isotype were detected in the sera of 24 patients by the method of indirect immunofluorescence, and 5 patients showed also the positivity of IgA isotype. In control samples these autoantibodies were not detected. Other autoantibodies were not demonstrated in either patient or control group. Conclusions. Of the parameters tested in noninfectious colitis, atypical-pANCA on ethanol-fixed granulocytes appears to be a suitable serological marker of food protein-induced proctitis/proctocolitis and suggests a possible involvement of an autoimmune mechanisms in the pathogenesis of this disease

Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study-0

according to pI, in the second dimension, 10% polyacrylamide slab gel (SDS) was used. After immunoblotting with MoAb TRO-tf-01, only one isoform (arrow) of transferrins (underlined) was labeled (B). Detected antigen was identified by MALDI-TOF analysis as human transferrin (TF, sequence coverage of 31%, Z-score of 2.31). Molecular weight is shown on the left.<p><b>Copyright information:</b></p><p>Taken from "Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study"</p><p>http://www.rbej.com/content/6/1/27</p><p>Reproductive biology and endocrinology : RB&E 2008;6():27-27.</p><p>Published online 2 Jul 2008</p><p>PMCID:PMC2459177.</p><p></p

Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study-4

according to pI, in the second dimension, 10% polyacrylamide slab gel (SDS) was used. After immunoblotting with MoAb TRO-tf-01, only one isoform (arrow) of transferrins (underlined) was labeled (B). Detected antigen was identified by MALDI-TOF analysis as human transferrin (TF, sequence coverage of 31%, Z-score of 2.31). Molecular weight is shown on the left.<p><b>Copyright information:</b></p><p>Taken from "Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study"</p><p>http://www.rbej.com/content/6/1/27</p><p>Reproductive biology and endocrinology : RB&E 2008;6():27-27.</p><p>Published online 2 Jul 2008</p><p>PMCID:PMC2459177.</p><p></p

Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study-2

and on extravillous cytotrophoblast cells (arrowhead). B) Control experiment without primary antibody shows no transferrin staining in the syncytium of normal term placenta. C) Very low expression of transferrin in the syncytium of normal term placenta. D) Placenta of mother with gestational diabetes shows strong villous expression of transferrin. E) Placenta of mother with pregnancy-induced hypertension shows strong expression of transferrin in villous syncytium. F) Placenta of drug-abuse mother during pregnancy; the transferrin expression is very strong. Hematoxylin counterstained the nuclei.<p><b>Copyright information:</b></p><p>Taken from "Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study"</p><p>http://www.rbej.com/content/6/1/27</p><p>Reproductive biology and endocrinology : RB&E 2008;6():27-27.</p><p>Published online 2 Jul 2008</p><p>PMCID:PMC2459177.</p><p></p

Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study-3

Ifferent placentae. NP, normal pregnancies; GD, gestational diabetes during pregnancy; PIH, pregnancy-induced hypertension during pregnancy; DrA, drug-abusing mothers during pregnancy. Statistical analysis has shown highly significant differences (p < 0.001) between normal (a) and each of the selected abnormal pregnancy types (b).<p><b>Copyright information:</b></p><p>Taken from "Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study"</p><p>http://www.rbej.com/content/6/1/27</p><p>Reproductive biology and endocrinology : RB&E 2008;6():27-27.</p><p>Published online 2 Jul 2008</p><p>PMCID:PMC2459177.</p><p></p