832 research outputs found
Photoelectron angular distributions in photodetachment from P-
The angular distributions of electrons ejected in laser photodetachment of the P- ion have been studied in the photon energy range of 0.95-3.28 eV using a photoelectron spectrometer designed to accommodate a source consisting of collinearly overlapping photon and negative ion beams. We observe the value of the asymmetry parameter β starting at zero near the threshold, falling to almost -1 about 0.5 eV above the threshold and eventually rising to a positive value. The experimental data has been fitted to a simplified model of the Cooper-Zare formula which yields a qualitative understanding of the quantum interference between the outgoing s and d waves representing the free electron. The present results are also compared with previous results for other elements involving p-electron photodetachment
Electron spin resonance of nitrogen-vacancy centers in optically trapped nanodiamonds
Using an optical tweezers apparatus, we demonstrate three-dimensional control
of nanodiamonds in solution with simultaneous readout of ground-state
electron-spin resonance (ESR) transitions in an ensemble of diamond
nitrogen-vacancy (NV) color centers. Despite the motion and random orientation
of NV centers suspended in the optical trap, we observe distinct peaks in the
measured ESR spectra qualitatively similar to the same measurement in bulk.
Accounting for the random dynamics, we model the ESR spectra observed in an
externally applied magnetic field to enable d.c. magnetometry in solution. We
estimate the d.c. magnetic field sensitivity based on variations in ESR line
shapes to be ~50 microTesla/Hz^1/2. This technique may provide a pathway for
spin-based magnetic, electric, and thermal sensing in fluidic environments and
biophysical systems inaccessible to existing scanning probe techniques.Comment: 29 pages, 13 figures for manuscript and supporting informatio
Molecular epidemiology of antiretroviral resistance in therapy-experienced HIV-1 patients in Cuba (2009)
Extreme Mass Ratio Inspirals: LISA's unique probe of black hole gravity
In this review article I attempt to summarise past and present-ongoing-work
on the problem of the inspiral of a small body in the gravitational field of a
much more massive Kerr black hole. Such extreme mass ratio systems, expected to
occur in galactic nuclei, will constitute prime sources of gravitational
radiation for the future LISA gravitational radiation detector. The article's
main goal is to provide a survey of basic celestial mechanics in Kerr spacetime
and calculations of gravitational waveforms and backreaction on the small
body's orbital motion, based on the traditional `flux-balance' method and the
Teukolsky black hole perturbation formalism.Comment: Invited review article, 45 pages, 23 figure
Gravitational waveforms from a point particle orbiting a Schwarzschild black hole
We numerically solve the inhomogeneous Zerilli-Moncrief and Regge-Wheeler
equations in the time domain. We obtain the gravitational waveforms produced by
a point-particle of mass traveling around a Schwarzschild black hole of
mass M on arbitrary bound and unbound orbits. Fluxes of energy and angular
momentum at infinity and the event horizon are also calculated. Results for
circular orbits, selected cases of eccentric orbits, and parabolic orbits are
presented. The numerical results from the time-domain code indicate that, for
all three types of orbital motion, black hole absorption contributes less than
1% of the total flux, so long as the orbital radius r_p(t) satisfies r_p(t)> 5M
at all times.Comment: revtex4, 24 pages, 23 figures, 3 tables, submitted to PR
Treatment Strategy with Gene Editing for Late-onset Retinal Degeneration Caused by a Founder Variant in C1QTNF5
AbstractPurpose: Genome editing is an emerging group of technologies with the potential to ameliorate dominant, monogenic human diseases such as late-onset retinal degeneration (L-ORD). The goal of this study was to identify disease stages and retinal locations optimal for evaluating the efficacy of a future genome editing trial.Methods: Twenty five L-ORD patients (age range, 33-77 years; median age, 59 years) harboring the founder variant S163R in C1QTNF5 were enrolled from three centers in the United Kingdom and United States. Patients were examined with widefield optical coherence tomography (OCT) and chromatic perimetry under dark-adapted and light-adapted conditions to derive phenomaps of retinal disease. Results were analyzed with a model of a shared natural history of a single delayed exponential across all subjects and all retinal locations.Results: Critical age for the initiation of photoreceptor loss ranged from 48 years at the temporal paramacular retina to 74 years at the inferior midperipheral retina. Subretinal deposits (sRET-Ds) became more prevalent as critical age was approached. Subretinal pigment epithelial deposits (sRPE-Ds) were detectable in the youngest patients showing no other structural or functional abnormalities at the retina. The sRPE-D thickness continuously increased, reaching 25 µm in the extrafoveal retina and 19 µm in the fovea at critical age. Loss of light sensitivity preceded shortening of outer segments and loss of photoreceptors by more than a decade.Conclusions: Retinal regions providing an ideal treatment window exist across all severity stages of L-ORD
Ambulatory care management of 69 patients with acute severe ulcerative colitis in comparison to 695 inpatients: insights from a multicentre UK cohort study
Black Hole Spectroscopy: Testing General Relativity through Gravitational Wave Observations
Assuming that general relativity is the correct theory of gravity in the
strong field limit, can gravitational wave observations distinguish between
black hole and other compact object sources? Alternatively, can gravitational
wave observations provide a test of one of the fundamental predictions of
general relativity? Here we describe a definitive test of the hypothesis that
observations of damped, sinusoidal gravitational waves originated from a black
hole or, alternatively, that nature respects the general relativistic no-hair
theorem. For astrophysical black holes, which have a negligible charge-to-mass
ratio, the black hole quasi-normal mode spectrum is characterized entirely by
the black hole mass and angular momentum and is unique to black holes. In a
different theory of gravity, or if the observed radiation arises from a
different source (e.g., a neutron star, strange matter or boson star), the
spectrum will be inconsistent with that predicted for general relativistic
black holes. We give a statistical characterization of the consistency between
the noisy observation and the theoretical predictions of general relativity,
together with a numerical example.Comment: 19 pages, 7 figure
Canine and Human Visual Cortex Intact and Responsive Despite Early Retinal Blindness from \u3cem\u3eRPE65\u3c/em\u3e Mutation
Background
RPE65 is an essential molecule in the retinoid-visual cycle, and RPE65 gene mutations cause the congenital human blindness known as Leber congenital amaurosis (LCA). Somatic gene therapy delivered to the retina of blind dogs with an RPE65 mutation dramatically restores retinal physiology and has sparked international interest in human treatment trials for this incurable disease. An unanswered question is how the visual cortex responds after prolonged sensory deprivation from retinal dysfunction. We therefore studied the cortex of RPE65-mutant dogs before and after retinal gene therapy. Then, we inquired whether there is visual pathway integrity and responsivity in adult humans with LCA due to RPE65 mutations (RPE65-LCA).
Methods and Findings
RPE65-mutant dogs were studied with fMRI. Prior to therapy, retinal and subcortical responses to light were markedly diminished, and there were minimal cortical responses within the primary visual areas of the lateral gyrus (activation amplitude mean ± standard deviation [SD] = 0.07% ± 0.06% and volume = 1.3 ± 0.6 cm3). Following therapy, retinal and subcortical response restoration was accompanied by increased amplitude (0.18% ± 0.06%) and volume (8.2 ± 0.8 cm3) of activation within the lateral gyrus (p \u3c 0.005 for both). Cortical recovery occurred rapidly (within a month of treatment) and was persistent (as long as 2.5 y after treatment). Recovery was present even when treatment was provided as late as 1–4 y of age. Human RPE65-LCA patients (ages 18–23 y) were studied with structural magnetic resonance imaging. Optic nerve diameter (3.2 ± 0.5 mm) was within the normal range (3.2 ± 0.3 mm), and occipital cortical white matter density as judged by voxel-based morphometry was slightly but significantly altered (1.3 SD below control average, p = 0.005). Functional magnetic resonance imaging in human RPE65-LCA patients revealed cortical responses with a markedly diminished activation volume (8.8 ± 1.2 cm3) compared to controls (29.7 ± 8.3 cm3, p \u3c 0.001) when stimulated with lower intensity light. Unexpectedly, cortical response volume (41.2 ± 11.1 cm3) was comparable to normal (48.8 ± 3.1 cm3, p = 0.2) with higher intensity light stimulation.
Conclusions
Visual cortical responses dramatically improve after retinal gene therapy in the canine model of RPE65-LCA. Human RPE65-LCA patients have preserved visual pathway anatomy and detectable cortical activation despite limited visual experience. Taken together, the results support the potential for human visual benefit from retinal therapies currently being aimed at restoring vision to the congenitally blind with genetic retinal disease
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