Agroecological prospects of using corn hybrids for biogas production

Ukraine is an agricultural country with great agricultural potential for biogas production, which is the key to fertile soils and favorable climatic conditions for energy crops, including corn. The article analyzes the experience of using biogas in Ukraine and the world, its mechanism of production. The leader in biogas production in the world is the European Union in general and Germany in particular. The total number of biogas plants in Europe exceeds 11 thousand, of which 7.2 thousand in Germany. Іnstalled biogas, which is released in the process of complex fermentation of organic waste, consists of a mixture of gases: methane – 55–75%, carbon dioxide – 23–33%, hydrogen sulfide – 7%. An important sector of renewable energy sources in biogas production is presented and the prospects for its use are determined. The energy dependence of our country on the volumes of imported natural gas is analyzed. The main aspects of biogas production are explored using renewable energy sources that are inexhaustible in our crane and the phased operation of the biogas plant is investigated. The real advantages of the need for biogas production and use in our country are outlined. Problems aimed at the development of alternative energy have been proved in order to detect environmental pollution. It has been established that in Ukraine the use of corn silage to improve the efficiency of biogas production at existing biogas stations has not been used so far. The problems of increasing the yield of corn plants have been proved not only by breeding and genetic methods, but also by cultivation technology

Development of three-enzyme lactose amperometric biosensor modified by nanosized poly (meta-phenylenediamine) film

© 2021, King Abdulaziz City for Science and Technology.New three-enzyme lactose amperometric biosensor based on platinum disc electrode modified by nanosized semipermeable poly (meta-phenylenediamine) film was developed in this work. It was studied the influence of different conditions of meta-phenylenediamine electropolymerization on the parameters (sensitivity and selectivity) of the amperometric transducers. The most optimal parameters were achieved after performing the electropolymerization of 5 mM meta-phenylenediamine applying 10–13 cyclic voltammograms. The bioselective element of lactose biosensor consisted of three enzymes (β-galactosidase, mutarotase, glucose oxidase) that were immobilized on the surface of modified working electrode. The analytical characteristics of the developed biosensor were studied. The linear range of lactose determination extended from 0.01 mM to 1.25 mM. The response time of the biosensor was 30 s. The biosensor showed high signal reproducibility (RSD = 1.16%), high sensitivity (LOD = 0.005 mM) and high selectivity. The proposed biosensor was tested for measurement of lactose concentration in milk samples. The high correlation between results obtained with lactose biosensor and HPLC was shown (R = 0.96%). Thus, the developed biosensor is suited for rapid, inexpensive, sensitive, selective and simple determination of lactose in milk samples