Role of bacteriophages from family Siphoviridae in lysogenic conversion of Bordetella bronchiseptica and their antimicrobial potential

U ovom radu su izolovani specifični  Bordetella bronchiseptica  bakteriofagi iz iz priodne sredine koji pripadaju familiji  Siphoviridae. Bakteriofagi su  okarakterisani u cilju razmatranja njihove uloge u lizogenoj konverziji bakterije i mogućnosti primene u kontroli vrste  B. bronchiseptica. U tu svrhu ispitivane su morfološke karakteristike odabranih faga, litički  spektar faga, i karakteristike genoma i proteina faga. Takođe, ispitivana je i stabilnost faga u različitim uslovima sredine, njihova litička efikasnost i efekat na formiranje i vec formirani biofilm. U radu je ispitana i uloga faga u produkciji biofilma,  rezistenciji na antibiotike, procesu hemolize i pokretljivosti kod vrste  B. bronchiseptica.  Rezultati ovog rada jasno ukazuju na ulogu faga u lizogenoj konverziji vrste  B.  bronchiseptica  kao  i mogućnost njihove primene, uz određene modifikacije, kao anti-B.bronchiseptica agenasa.In this paper Bordetella bronchiseptica specific phages belonging to family Siphoviridae were isolated from environment. Bacteriophages were characterized to determine their role in the lysogenic conversion of bacteria and their possible usage in B. bronchiseptica control. For this purpose, morphological characteristics of the selected phages, the phage lytic spectrum, and the characteristics of phage genome and the proteins were examined. Also, the stability of phage in different environmental conditions was studied as well as their lytic efficiency, effect on the biofilm formation and formed biofilm. The paper also examined the role of phages in the production of biofilm, resistance to antibiotics, the process of hem olysis and the motility of B. bronchiseptica species. The results of this study clearly indicate the role of phage in lysogenic conversion in B. bronchiseptica as well as their potential for B. bronchiseptica control

Tri nova posmatranja debelokljune čigre Sterna nilotica

Gull-billed Tern Sterna nilotica represent irregular passage bird of Serbia. During the 2010 this species was recorded 3 times – on 29th April, 17th July and 4th August respectively. In all occasion one adult bird was observed feeding or resting during shorter or longer period of times. On all localites Gull-billed Tern was not recorded in the past. These sites are waster water lakes of sugar plant „Kovačica“, waste ponds between the town Pančevo and village Starčevo and Special nature reserve „Zasavica“

Role of bacteriophages from family Siphoviridae in lysogenic conversion of Bordetella bronchiseptica and their antimicrobial potential

U ovom radu su izolovani specifični  Bordetella bronchiseptica  bakteriofagi iz iz priodne sredine koji pripadaju familiji  Siphoviridae. Bakteriofagi su  okarakterisani u cilju razmatranja njihove uloge u lizogenoj konverziji bakterije i mogućnosti primene u kontroli vrste  B. bronchiseptica. U tu svrhu ispitivane su morfološke karakteristike odabranih faga, litički  spektar faga, i karakteristike genoma i proteina faga. Takođe, ispitivana je i stabilnost faga u različitim uslovima sredine, njihova litička efikasnost i efekat na formiranje i vec formirani biofilm. U radu je ispitana i uloga faga u produkciji biofilma,  rezistenciji na antibiotike, procesu hemolize i pokretljivosti kod vrste  B. bronchiseptica.  Rezultati ovog rada jasno ukazuju na ulogu faga u lizogenoj konverziji vrste  B.  bronchiseptica  kao  i mogućnost njihove primene, uz određene modifikacije, kao anti-B.bronchiseptica agenasa.In this paper Bordetella bronchiseptica specific phages belonging to family Siphoviridae were isolated from environment. Bacteriophages were characterized to determine their role in the lysogenic conversion of bacteria and their possible usage in B. bronchiseptica control. For this purpose, morphological characteristics of the selected phages, the phage lytic spectrum, and the characteristics of phage genome and the proteins were examined. Also, the stability of phage in different environmental conditions was studied as well as their lytic efficiency, effect on the biofilm formation and formed biofilm. The paper also examined the role of phages in the production of biofilm, resistance to antibiotics, the process of hem olysis and the motility of B. bronchiseptica species. The results of this study clearly indicate the role of phage in lysogenic conversion in B. bronchiseptica as well as their potential for B. bronchiseptica control

Do <i>Ganoderma</i> Species Represent Novel Sources of Phenolic Based Antimicrobial Agents?

Ganoderma species have been recognized as potential antimicrobial (AM) agents and have been used in traditional Chinese medicine (TCM) for a long time. The aim of this study is to examine the AM potential of autochthonous Ganoderma species (G. applanatum, G. lucidum, G. pfeifferi and G. resinaceum) from Serbia. The extraction of fungal material was prepared in different solvents (ethanol—EtOH, water—H2O, chloroform—CHCl3). Antibacterial activity (ABA) was determined using disk-diffusion, agar-well diffusion, and micro-dilution method, while for antifungal properties disk-diffusion and pour plate method were applied. Antiviral activity was tested on model DNA virus LK3 and determined by plaque assay. Statistical PCA analysis was applied for detection of correlation effects of phenolics and AM activities, while LC-MS/MS was performed for phenolics quantification. G. resinaceum CHCl3 extract expressed the most potent ABA against P. aeruginosa (MIC = 6.25 mg/mL), probably due to presence of flavonoids and 2,5-dihydroxybenzoic acid. Among H2O extracts, the highest ABA was determined for G. pfeifferi against both E. coli and S. aureus (21 and 19 mm, respectively). EtOH extracts of G. pfeifferi and G. resinaceum were the most effective against A. niger (23.8 and 20.15 mm, respectively), with special impact of phenolic acids and flavonoid isorhamnetin, while C. albicans showed the lowest susceptibility. The most potent antiviral inhibitor was G. lucidum (70.73% growth inhibition) due to the high amount of phenolic acids. To the best of our knowledge, this is the first report of a methodical AM profile of G. pfeifferi and G. resinaceum from the Balkan region including PCA analysis