Analisis mutacional del gen de la presenilina-1 y su asociacion con la enfermedad de Alzheimer

Centro de Informacion y Documentacion Cientifica (CINDOC). C/Joaquin Costa, 22. 28002 Madrid. SPAIN / CINDOC - Centro de Informaciòn y Documentaciòn CientìficaSIGLEESSpai

Effects of oxidative stress on HSV-1 protein expression.

<p>The accumulation of viral proteins ICP4, VP16 and gC was analysed by immunoblotting in SK-N-MC cells simultaneously treated with X-XOD and infected with HSV-1 at a moi of 1 and 10 for 18 h (<b>A</b>) or at a moi of 0.1 for 42 h (<b>B</b>). The blots shown are representative of three independent experiments. A tubulin blot was performed as a loading control. In all blots, the ratio of viral proteins to tubulin is shown below the blots.</p

Oxidative stress reduces HSV-1 replication and increases cell viability of infected cells.

<p><b>A)</b> Quantification of viral DNA by real-time quantitative PCR in SK-N-MC cells simultaneously treated with X-XOD and infected with HSV-1 at a moi of 1 and 10 for 18 h or at a moi of 0.1 for 42 h. <b>B)</b> Intracellular (intra) and extracellular (extra) viral titres were determined by plaque assays in SK-N-MC cells infected under the same conditions as in (<b>A</b>). In (<b>A</b>) and (<b>B</b>), the data represent the mean ± SEM of five experiments performed in triplicate and are expressed as a percentage with respect to untreated cells (- X-XOD) (*p<0.05; **p<0.01; ***p<0.001). <b>C)</b> The cell viability of mock and HSV-1-infected SK-N-MC cells exposed to X-XOD was monitored using the MTT reduction assay. Cells were infected with HSV-1 at a moi of 1 and 10 for 18 h or at a moi of 0.1 for 42 h. Values are expressed relative to the optical density of untreated mock-infected cells. The data shown represent the mean ± SEM for four independent experiments performed in triplicate (*p<0.05; **p<0.01).</p

Effects of oxidative stress on HSV-1 entry.

<p><b>A)</b> Immunofluorescence analysis of HSV-1-infected SK-N-MC cells at a moi of 10 in the presence and absence of X-XOD. The immunoreactivity of ICP4 protein is shown at 3 and 5 hours post-infection (h.p.i.). Nuclei are stained with DAPI. Scale bar: 20 µm. <b>B)</b> Quantification of infected cells by ICP4 staining. The graph shows the percentage of ICP4-positive cells. At least 400 nuclei were counted for each condition. <b>C)</b> Analysis of ICP4 levels by Western blotting in SK-N-MC cells infected with HSV-1 at a moi of 1 and 10 for 3 h and 5 h, in the presence and absence of X-XOD.</p

Oxidative stress does not affect the autophagosomal localization of Aβ in HSV-1-infected cells.

<p>Confocal images obtained with anti-Aβ and anti-LC3 antibodies showing endogenous LC3 and Aβ patterns in HSV-1-infected SK-APP cells at a moi of 10 for 18 h in the presence and absence of oxidative stress (X-XOD). Colocalization is shown by yellow fluorescence signals in the merged panels. Scale bar: 10 µm.</p

Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

non present