Curcumin ameliorates experimental autoimmune acute myocarditis in rats as evidenced by decrease in thioredoxin immunoreactivity

This study was performed to investigate the effect of curcumin on cardiac myosin-induced autoimmune myocarditis in rats and the change in thioredoxin (TRX) immunoreactivity in cardiomyocytes following curcumin treatment. Twenty-four six-week-old male Wistar rats were randomly allocated into 4 groups of 6 rats each. Group I received neither curcumin nor myosin. Group II received an oral solution of 1 g/kg/day of curcumin daily, from day 1 to day 21. To induce myocarditis, animals of both group III and group IV were injected by 1 mg of porcine cardiac myosin on days 1 and 8. In addition, animals of group IV received an oral solution of 1 g/kg/day of curcumin daily, from day 1 to day 21. Serum levels of creatine phosphokinase, troponin-T, tumour necrosis factor-alpha and interleukin-6 were estimated. Hearts were processed for histopathological and immunohistochemical studies. Serum biomarkers levels were significantly increased in myocarditis group as compared to other groups. The intake of curcumin significantly reduced the deviation in these markers. Sections of the wall of the heart from myocarditis group were characterised by inflammatory cell infiltration. Most of cardiomyocytes showed pyknotic nuclei and increased sarcoplasmic eosinophilia with strong immunoreactivity for TRX. Sections from myocarditis-curcumin group showed normal architecture with moderate immunoreactivity for TRX. The present study demonstrated that curcumin ameliorates acute myocarditis in rats and encouraged the estimation of serum level of TRX as a relevant indicator for the evaluation of the progress of acute myocarditis

Stem cells from human extracted deciduous teeth expanded in foetal bovine and human sera express different paracrine factors after exposure to freshly prepared human serum

Background: The response of stem cells to paracrine factors within the host’s body plays an important role in the regeneration process after transplantation. The aim of this study was to determine the viability and paracrine factor profile of stem cells from human extracted deciduous teeth (SHED) pre-cultivated in media supplemented with either foetal bovine serum (FBS) or pooled human serum (pHS) in the presence of individual human sera (iHS). Methods: SHED (n=3) from passage 4 were expanded in FBS (FBS-SHED) or pHS (pHS-SHED) supplemented media until passage 7. During expansion, the proliferation of SHED was determined. Cells at passage 7 were further expanded in human serum from four individual donors (iHS) for 120 hours followed by assessment of cell viability and profiling of the secreted paracrine factors. Results: Proliferation of SHED was significantly higher (p<0.05) in pHS supplemented media compared to FBS supplemented media. pHS-SHED also maintained their higher proliferation rate compared to FBS-SHED in the presence of iHS. In iHS supplemented media, FBS-SHED expressed significantly higher levels of SDF-1A (p<0.05) after 24 hours compared to pHS-SHED. Similar results were found for HGF (p<0.01), LIF (p<0.05), PDGF-BB (p<0.05), SDF-1A (p<0.01), and IL-10 (p<0.05) when cell culture supernatants from FBS-SHED was profiled 120 hours post-incubation. Conclusion: SHED expanded in pHS instead of FBS have higher proliferative capacity and show an altered secretion profile. Further studies are needed to determine whether these differences could result in better engraftment and regeneration following transplantation

Saudi Arabian science and mathematics teachers’ attitudes toward integrating STEM in teaching before and after participating in a professional development program

For the advancement of current and future economic and overall well-being to occur, STEM professionals are highly needed. STEM education is an approach that integrates science, technology, engineering and mathematics curricula into one interdisciplinary curriculum. In the context of STEM education, learners acquire deep and critical thinking abilities, which help them become creative and analytic thinkers, and hence innovators in their careers. For this to happen, the teacher must be trained to successfully design and effectively teach an integrated STEM curriculum. The purpose of this study was to analyze Saudi Arabian science and mathematics teachers’ attitudes toward integrating Science, Technology, Engineering and Mathematics (STEM) in teaching before and after they participated in a short professional development program focused on STEM integration in a specific middle school science and mathematics content. The participants were 48 Saudi Arabian science and mathematics teachers, who participated in a 6-day program held in Riyadh, Saudi Arabia. The research methodology was a pretest–posttest one group (pre-experimental) design. The primary data source was the Instrument for Teachers’ Attitudes Toward Teaching Integrated STEM. The results indicate that Saudi Arabian science and mathematics teachers’ perceptions of difficulties decreased due to their participation in the professional development program on integrated STEM. Meanwhile, the teachers’ self-efficacy improved following their participation in the STEM professional development (PD) program. However, no perceived effect was found for the teachers’ perceptions of the relevance of or their anxiety about or enjoyment of Integrated STEM teaching due to their participation in the 6-day PD program. We argue that Engaging teachers in integrated STEM professional development may improve their attitudes toward and increase their interest in teaching this approach. To improve teachers’ attitudes toward, it is suggested that future PD programs should be long and continuous, and focus on training science and mathematics teachers to teach integrated STEM subjects

Content Analysis of Essential Economic Values in the Vocational Textbook as Compared to the Saudi Arabian Education Policy Document

This study explores the extent to which five essential economic values related to the themes of production, savings, moderation in expenditure, labor, and economic development were included in the Saudi Arabian Educational Policy Document and their reflection in a high school vocational textbook. A systematic quantitative descriptive approach was used to analyze the contents of the two documents. Results showed that the Education Policy Document only included the production domain value. The vocational textbook did not significantly include all economic values, except the theme related to respecting and the value of time. This means that the two documents should be revised to include these important economic values, as they are important for students’ future