53 research outputs found
Assembly of <i>Francisella novicida</i> Cpf1 endonuclease in complex with guide RNA and target DNA
“Realización de Proyecto de Sendero Turístico de Aragón: Ruta de los barrancos (San Blas–Valdelobos)(Huérmeda - Calatayud)”
El siguiente Trabajo Fin de Grado trata de la realización a través de las áreas de senderismo y turismo, junto con la elaboración de un proyecto técnico para el trazado de una ruta en un área rural concreta. Se conseguirá que los usuarios de este sendero conozcan que se trata de una realización de un sendero de Interés Turístico Regional. Para ello se expone un proyecto técnico, el cual incluye el trazado de la ruta circular preferentemente pedestre, y los objetivos y contenidos del mismo. Además se detalla la metodología llevada a cabo tras las salidas de campo para la realización del proyecto técnico, incluyendo los anexos convenientes para su desarrollo.<br /
FANCD2–FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair
Vertebrate DNA crosslink repair excises toxic replication-blocking DNA crosslinks. Numerous factors involved in crosslink repair have been identified, and mutations in their corresponding genes cause Fanconi anemia (FA). A key step in crosslink repair is monoubiquitination of the FANCD2-FANCI heterodimer, which then recruits nucleases to remove the DNA lesion. Here, we use cryo-EM to determine the structures of recombinant chicken FANCD2 and FANCI complexes. FANCD2-FANCI adopts a closed conformation when the FANCD2 subunit is monoubiquitinated, creating a channel that encloses double-stranded DNA (dsDNA). Ubiquitin is positioned at the interface of FANCD2 and FANCI, where it acts as a covalent molecular pin to trap the complex on DNA. In contrast, isolated FANCD2 is a homodimer that is unable to bind DNA, suggestive of an autoinhibitory mechanism that prevents premature activation. Together, our work suggests that FANCD2-FANCI is a clamp that is locked onto DNA by ubiquitin, with distinct interfaces that may recruit other DNA repair factors
Complex Impedance Transformers Based on Branch-Line Hybrid Couplers
A topology, equations and design methodology for complex impedance-transforming branch-line hybrid couplers are presented. This method also allows the realization of real impedancetransforming to higher impedances. Limitations for real, imaginary and complex impedances are discussed. Test results are shown for a 3 dB 50 to 450 Ω hybrid coupler, at a 2 GHz center frequency, with a 21% bandwidth, an amplitude balance of 4.35 ± 1 dB and a phase balance of 92.16◦ ± 8.8◦. To showcase the complex impedance scenario, two 3 dB 50 Ω to 70 − 200j Ω are measured at a 2 GHz center frequency. One of these couplers uses a technique for reducing the chip size, yielding a 22.5% bandwidth, 4 ± 0.9 dB amplitude balance and 93.22◦ ± 6.74◦ phase balance, while accomplishing a 25% size reductio
Comparison of the near-field measurements between a commercial open-ended rectangular waveguide probe and its equivalent probe in siw technology
2015 AMTA 37th Annual Meeting & Symposiu
Substrate Integrated Waveguides Structures using Frequency Selective Surfaces operating in Stop-Band (SBFSS-SIW)
A novel Substrate Integrated Waveguide (SIW) is proposed using Frequency Selective Surfaces (FSS) operating in Stop- Band (SBFSS-SIW) as metal conductor plates. These structures preserve the propagation properties and the dimensions of the conventional SIW, and have periodic isolated pads that could allow the direct integration of biased active and passive devices on the SIW surface. A set of SBFSS-SIW for X-band have been designed, simulated and experimentally measured to demonstrate that SBFSS-SIWs can behave similar to conventional SIW structures when FSS cells are optimized to work far from its resonance frequencyThis work was supported by the Gobierno del Principado de Asturias under the Plan de Ciencia Tecnología e Innovación (PCTI) by grants BP13042 and BP12032, by the Gobierno del Principado de Asturias and FEDER (Unión Europea) under project GRUPIN14-114, and the Ministerio de Economía y Competitividad under project TEC2014-54005-
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