Antimicrobial resistance of bacterial strains isolated from avian cellulitis

Avian cellulitis is an inflammatory process in the subcutaneous tissue, mainly located in the abdomen and thighs. This problem is commonly observed in poultry at slaughter and it is considered one of the major causes of condemnation of carcasses in Brazil. The aim of this study was to perform the microbial isolation of lesions of avian cellulitis from a processing plant located in the State of Goiás in order to analyze antimicrobial resistance by antibiogram test and to detect resistance genes by polymerase chain reaction. A total of 25 samples of avian cellulitis lesions were analyzed, from which 30 bacterial strains were isolated. There were eleven (44%) strains of Escherichia coli, nine (36%) strains of Staphylococcus epidermidis, seven (28%) strains of Proteus mirabilis and three (12%) strains of Manheimiahaemolytica. The antibiogram test showed that all strains were resistant to at least one antimicrobial. The gene of antimicrobial resistance tetB was detected in E. coli, S. epidermidis and P. mirabilis strains, and was the most frequently observed gene. The gene of antimicrobial resistance Sul1 was detected in all bacterial species, while tetA was found in E. coli and S. epidermidis strains, SHV in E. coli strains, S. epidermidis and P. mirabilis,and cat1 in one P. mirabilis strain. The results suggest a potential public health hazard due to the ability of these microorganisms to transmit antimicrobial resistancegenes to other microorganisms present in the intestinal tract of humans and animals, which may affect clinical-medical usage of these drugs

Realization of antibiogram an detection of antibiotic resistance genes by polymerase chain reaction (PCR) in strains of Escherichia coli isolated from avian cellulitis, collected from slaughterhouses in Distrito Federal

Monografia (graduação)—Universidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, 2011.O objetivo deste trabalho foi observar o perfil de resistência antimicrobiana de cepas de Escherichia coli de celulite aviária. Foram isoladas 22 cepas de E. coli coletadas de frangos de corte de abatedouros localizados no Distrito Federal. Todas as amostras foram investigadas quanto à susceptibilidade a 16 agentes antimicrobianos pelo método de disco difusão. A pesquisa de genes de resistência foi feita pela técnica da PCR, por multiplex e uniplex. Todas as amostras apresentaram no antibiograma resistência a pelo menos dois antimicrobianos. Os maiores níveis de resistência foram encontrados para penicilina (100%), espiramicina (100%), sulfonamida (90,91%) e eritromicina (81,82%). Na investigação dos genes de resistência, os mais detectados foram sul1 (68,18%), ereA (50%) e cat1 (36,36%). Apenas em uma amostra não foi detectado nenhum dos genes de resistência pesquisados. Este estudo revelou que a presença de bactérias resistentes a antibióticos em alimentos destinados ao consumo humano, como a carne de frango, pode se mostrar um risco para a saúde pública. _______________________________________________________________________________ ABSTRACTThe aim of this work was to observe the antimicrobial resistance profile of Escherichia coli strains from avian cellulitis. Twenty-two E. coli isolates were collected from broilers from slaughterhouses at Federal District area. All the samples were investigated to their susceptibility to 16 antimicrobial agents by disk diffusion method. The search for resistance genes was performed by PCR multiplex and uniplex. All the samples showed antibiotic resistance to at least two antimicrobials. The highest levels of resistance were found for penicillin (100%), spiramycin (100%), sulfonamide (90.91%) and erythromycin (81.82%). In the resistance genes investigation, the genes most frequently detected were sul1 (68.18%), ereA (50%) and cat1 (36.36%). Only in one sample was not detected none of the resistance genes studied. This study revealed that the presence of antibiotic resistant bacteria in food for human consumption, such as chicken, may have a risk to public health