Fraksinasi dan Identifikasi SenyawaAntioksidan pada Eksb-ak Etanol buah Goji beny(Lycium barbarum linn.) secaraKromatografi Kolom

Goji berry fruit (Lycium barbarum), familia Solanac'eae, is a red berry fruit. The chemical compounds contained in this fruit are polysaccharides, zeaxanthin and beta-carotene. Activity of the chemical compounds is still considered as a family of antioxidant compounds, which are used as a medicine to cure the diseases caused by the presence of free radicals such a~ atherosclerosis, cancer, liver cirrhosis, hypertension and diabetes. The research will be carried on fractionation and identification of the ethanol extract of Goji berry fruit to obtain compounds that have antioxidant power using chromatographic column method and DPPH method. The isolated compounds will be identified using methods phytochemica/s screening, TLC, UV-Vis spectrophotometer and infrared spectrophotometer. The result showed that secondary metabolites of Goji berry fruit which are potential as antioxidant compound were flavonoid (flavono/s or flavones). The isolated compound had higher antioxidant activity compare to the ethanol extract of Goji berry fruit Flavonoids are the major compounds that have antioxidant capacity. Whereas, the ethanol fraction of Goji berry fruits has the best antioxidant activity than the ethanol extract Keywords: Antioxidant, Goji berry, DPPH and chromatographic colum

Efektivitas dan Keamanan Furosemid Continuous Infusion Dosis 10 dan 20 mg/jam pada Pasien Penyakit Ginjal Kronik Kondisi Fluid Overload Syndrome disertai Hipoalbumin di RSUD Abdul Wahab Sjahranie

Furosemide continuous infusion is the first choice drug in overcoming the condition of Fluid overload syndrome with hypoalbuminemia in patients with Chronic Kidney Disease (CKD). However, in these conditions, the dose of furosemide used by clinicians is between 10 and 20 mg/hour. No studies have compared the effectiveness and safety of different doses of furosemide. This study aimed to evaluate the effectiveness and safety of 10 and 20 mg/hour furosemide given in continuous infusion in CKD patients with fluid overload syndrome with hypoalbuminemia. The method is a Single-blind, Randomized Clinical Trial conducted during the period of February-April 2017 in the inpatient ward of Abdul Wahab Sjahranie Hospital. Data processing was carried out by statistical analysis, Two Independent T-Test parametric test for data that normally distributed and those that did not correlate with each other, whereas for data that not normally distributed and those that did not correlate with each other, non-parametric test Mann Whitney and Wilcoxon were used. Outcomes studies were 24-hour urine volume, shortness of breath, duration of oxygen consumption, Ronchi and safety of therapy seen from drug side effects (hypotension, hyperuricemia, hyperglycemia, hyponatremia, hypokalemia). The results of the study, 34 patients with CKD with fluid overload syndrome with hypoalbuminemia (2.5-3.0 g/dL) were registered. On the effectiveness of therapy, there was no significant difference in 24-hour urine volume (P = 0.324; P> 0.05; 95%; CI=714.6-1017.8 ml group 1; CI=818.0-1113.2 ml group 2), shortness of breath (P = 0.781; P> 0.05; 95 % CI=0.68-1.32 group 1; CI=0.72-1.40 group 2), duration of oxygen use (P = 0.363; P> 0.05; 95% CI=1.34-2.66 day group 1; CI=1.72-3.10 day group 2), Ronk (P = 0.692; P> 0.05 ) and the absence of drug adverse events (P = 1.000; P> 0.05) between the two groups. In conclusion, the dose of 10 and 20 mg/hour furosemide has the sam

Fraksinasi dan identifikasi senyawa antioksidan pada ekstraketanol buah goji berry (Lycium Barbarum Linn) secara kolom kromatografi. [CD-ROM]

Buah Goji berry (Lycium barbarum L), familia Solanaceae merupakan buah berry yang berwarna merah. Kandungan senyawa kimia yang dimiliki oleh buah Goji berry ini antara lain polisakarida, zeaxanthin dan beta-carotene. Aktivitas dari senyawa kimia tersebut masih tergolong keluarga dari senyawa antioksidan, yang digunakan sebagai terapi penyembuhan terhadap penyakit akibat dari adanya radikal bebas seperti penyakit atherosclerosis, cancer, liver cirrhosis, hypertension dan diabetes. Oleh karena itu, akan dilakukan penelitian tentang fraksinasi dan identifikasi terhadap ekstrak etanol buah Goji berry untuk mendapatkan senyawa atau golongan yang mempunyai daya antioksidan dengan metode kolom kromatografi dan metode DPPH. Senyawa yang terisolasi akan diidentifikasi dengan menggunakan metode skrinning fitokimia, KLT, Spektrofotometer UV-Vis dan spektrofotometer Inframerah. Dari hasil penelitian didapatkan bahwa buah Goji berry memiliki senyawa metabolit sekunder yang berfungsi sebagai antioksidan yaitu senyawa flavonoid (flavon atau flavonol). Aktivitas antioksidan hasil fraksinasi memberikan harga IC50 yang tinggi dibandingkan dengan ekstrak etanol buah Goji berry

Fraksinasi dan Identifikasi Senyawa Antioksidan pada Ekstrak Etanol buah Goji berry (Lycium barbarum Linn) secara Kromatografi Kolom.

Buah Goji berry (Lyc/um barbarum L). Memiliki kandungan senyawa kimia yang dimiliki antara lain polisakarida, zeaxanthin dan beta-carotene. Aktivitas dan senyawa kimia terse but masih tergolong keluarga dari 'senyawa a ntioksidan, yang digunakan sebagai terapi penyembuhan terhadap penyakit akibat dari adanya radikal bebas seperti penyakit atherosclerosis, cancer, liver cirrhosis, hipertensi dan diabetes. Pada penelitian ini dllakukan fraksinasi dan identifikasi terhadap ekstrak etanol buah Goji berry untuk mendapatkan senyawa atau golongan yang mempunyai daya antioksidan pada ekstrak etanol buah Goji berry dengan metode kolom kromatografi dan metod,e DPPH, Senyawa yang terisolasi akan diidentifikasi dengan menggunakan metode skrinning fitokimia, KLT, Spektrofotometer UV-Vis dan spektrofotometer Inframerah. Dari hasil penelitian didapatkan bahwa buah Goji berry memiliki senyawa metabolit sekunder yang berfungsi sebagai antioksidan yaitu senyawa flavonoid (flavon atau flavonol), Aktivitas antioksidan hasil fraksinasi memberikan aktivitas antioksidan yangtinggi dibandingkan dengan ekstrak etanol buah Goji berry. Golongan metabolit sekundar dalam fraksi etanol buah goji berry yang dapat berfungsi sebagai senyawa antioksidan adalah flavonoid dan fraksi etanol dari buah goji berry memiliki aktivitas antioksidan yang lebih besardibandingkan dengan ekstrak etanolnya

Fraksinasi dan Identifikasi SenyawaAntioksidan pada Eksb-ak Etanol Buah Goji Beny(Lycium Barbarum Linn.) SecaraKromatografi Kolom

Goji berry fruit (Lycium barbarum), familia Solanac'eae, is a red berry fruit. The chemical compounds contained in this fruit are polysaccharides, zeaxanthin and beta-carotene. Activity of the chemical compounds is still considered as a family of antioxidant compounds, which are used as a medicine to cure the diseases caused by the presence of free radicals such a~ atherosclerosis, cancer, liver cirrhosis, hypertension and diabetes. The research will be carried on fractionation and identification of the ethanol extract of Goji berry fruit to obtain compounds that have antioxidant power using chromatographic column method and DPPH method. The isolated compounds will be identified using methods phytochemica/s screening, TLC, UV-Vis spectrophotometer and infrared spectrophotometer. The result showed that secondary metabolites of Goji berry fruit which are potential as antioxidant compound were flavonoid (flavono/s or flavones). The isolated compound had higher antioxidant activity compare to the ethanol extract of Goji berry fruit Flavonoids are the major compounds that have antioxidant capacity. Whereas, the ethanol fraction of Goji berry fruits has the best antioxidant activity than the ethanol extrac

Pre-meal high-performance inulin supplementation reduce post-prandial glycaemic response in healthy subjects: A repeated single-arm clinical trial

Background and aims: High-performance (HP) inulin, a dietary fiber consists of more than 10 fructose polymers, have been shown to reduce post-prandial glycaemic response (PPGR) and could prevent the occurrence of Type-2 diabetes mellitus (T2DM). Currently, there are no data on whether pre-meal HP inulin supplementation could decrease PPGR. Methods: 8 healthy adults consumed 20 g of formula that contain 60.2% inulin (w/w) dissolved in water. Blood glucose was measured in fasted participants and at 30e120 min after starting to eat a prepared meal. This test was repeated every week with different supplement formulas. Conclusion: pre-meal HP Inulin formula supplementation could suppress the post-prandial glycaemic response

Phago pharmacology on the go. gamification approach in case learning process

Learning process among medical students have developed along with technological advances so that it can be useful for users. One of the breakthroughs in the world of medical education is the development of serious games using multimedia technology that are relatively small in size so that they can be accessed using computers or devices named Pharmacology on the Go (PHAGO). The implementation stage was done by testing prototype game on 88 users since October 20, 2021. Evaluation was done by using a feedback questionnaire for PHAGO users, shows 79 (89.8%) users who agree and strongly agree that the PHAGO application helps users understand the use of Pharmacology for clinical application. The results of the Wilcoxon test in evaluating the increase in students' knowledge with Pre Test and Post Test showed that there was a significant difference (p = 0.000). The existence of serious game stimulates an active learning process to create a learning environment that encourages the development of interpersonal, communication and problem solving skills. PHAGO is expected to be a forum that supports students to be able to independently study pharmacology in a fun and easy-to-understand way

The combination of isomalto-oligosaccharides (IMO)-based dietary fiber and hypocaloric high-protein diet could improve the anthropometric profile and fasting plasma glucose of healthy adults: A repeated single-arm clinical trial

Background and aims: Meals with high protein and fiber could reduce weight and improve diabetes risk factors. Isomalto-oligosaccharide (IMO), a form of dietary fiber, could induce the afferent signal that causes appetite suppression. However, the direct effect of fiber supplementation in the form of IMO combined with a high-protein diet (HPF) on those parameters is still unknown. This study aims to investigate the effect of HPF on anthropometric parameters and blood glucose regulation of healthy subjects. Methods: Thirteen healthy subjects were given a hypocaloric high protein diet (HPD) mixed with their prepared meals for two weeks. Followed by the HPF diet for another two weeks. Their anthropometric parameters, such as body composition (total body weight, body fat percentage, and fat-free mass), BMI and waist circumference, and fasting plasma glucose, were measured. Results: Compared to pre-intervention, HPF could significantly (p ≤ 0.004) reduce the anthropometric parameters and fasting plasma glucose. Compared to HPD, HPF could significantly (p ≤ 0.005) reduce more total body weight, body fat percentage, and BMI. In addition, HPF could induce more satiety than HPD (higher VAS score). Conclusion: HPF could improve the subject's anthropometric parameters which is obviously beneficial in preventing the risk of developing diabetes. © 2022 The Author(s

The combination of isomalto-oligosaccharides (IMO)-based dietary fiber and hypocaloric high-protein diet could improve the anthropometric profile and fasting plasma glucose of healthy adults: A repeated single-arm clinical trial

Background and aims: Meals with high protein and fiber could reduce weight and improve diabetes risk factors. Isomalto-oligosaccharide (IMO), a form of dietary fiber, could induce the afferent signal that causes appetite suppression. However, the direct effect of fiber supplementation in the form of IMO combined with a highprotein diet (HPF) on those parameters is still unknown. This study aims to investigate the effect of HPF on anthropometric parameters and blood glucose regulation of healthy subjects. Methods: Thirteen healthy subjects were given a hypocaloric high protein diet (HPD) mixed with their prepared meals for two weeks. Followed by the HPF diet for another two weeks. Their anthropometric parameters, such as body composition (total body weight, body fat percentage, and fat-free mass), BMI and waist circumference, and fasting plasma glucose, were measured. Results: Compared to pre-intervention, HPF could significantly (p ≤ 0.004) reduce the anthropometric parameters and fasting plasma glucose. Compared to HPD, HPF could significantly (p ≤ 0.005) reduce more total body weight, body fat percentage, and BMI. In addition, HPF could induce more satiety than HPD (higher VAS score). Conclusion: HPF could improve the subject’s anthropometric parameters which is obviously beneficial in preventing the risk of developing diabetes

PHAGO PHARMACOLOGY ON THE GO. GAMIFICATION APPROACH IN CASE LEARNING PROCESS

Learning process among medical students have developed along with technological advances so that it can be useful for users. One of the breakthroughs in the world of medical education is the development of serious games using multimedia technology that are relatively small in size so that they can be accessed using computers or devices named Pharmacology on the Go (PHAGO). The implementation stage was done by testing prototype game on 88 users since October 20, 2021. Evaluation was done by using a feedback questionnaire for PHAGO users, shows 79 (89.8%) users who agree and strongly agree that the PHAGO application helps users understand the use of Pharmacology for clinical application. The results of the Wilcoxon test in evaluating the increase in students' knowledge with Pre Test and Post Test showed that there was a significant difference (p = 0.000). The existence of serious game stimulates an active learning process to create a learning environment that encourages the development of interpersonal, communication and problem solving skills. PHAGO is expected to be a forum that supports students to be able to independently study pharmacology in a fun and easy-to-understand way