Average full-body gene expression patterns of r-opsin1, hdc and VAChT

RGB tiff stack of average registered full-body gene expression patterns of eye marker gene r-opsin1 (red) and neurotransmitter marker genes hdc (green) and VAChT (blue), created by RNA whole mount in situ hybridization in 3-day-old Platynereis larvae. Note that signal present in spinning glands, parapodia and chaetae of larvae is background

acetylated tubulin staining registered to the unbiased nuclear reference

Tiff stack of average acetylated tubulin staining registered to the unbiased nuclear reference of 3-day-old Platynereis larvae. The antibody labels cilia and the axonal scaffold (white)

Double in situ hybridization for r-opsin1 and hdc

RGB tiff stack of raw data from high-resolution apical scan of double RNA whole mount in situ hybridization with r-opsin1 (red) and hdc (green) in 3-day-old Platynereis larvae. Larva is counterstained with acetylated tubulin antibody (blue) to show cilia and axonal scaffold

Phototaxis videos of control and mecamylamine-treated larvae

Videos of phototaxis experiments. 3-day-old control larvae and larvae treated with 50 μm mecamylamine were imaged with 15 frames per second in a split phototaxis assay cuvette. The length of the cuvette is 15 mm. Directional white stimulus light was provided from the left. Each video is 600 frames. The videos are in Zeiss zvi format. The files were compressed with tar -jcvf archive_name.tar.bz2 directory_with_files/*, use tar –jxvf to uncompress them

Phototaxis ImageJ Macro

ImageJ macro used to score phototaxis videos

Blender file containing the complete virtual anatomical atlas of the Platynereis eye circuitry

Simplified 3D models of all neurons, synapses, muscles and ciliated cells are included. The file can be viewed with the Blender software http://www.blender.org/

Node ranking in the Platynereis eye circuitry

Node ranking in the Platynereis eye circuitry based on various centrality measures

Average full-body gene expression patterns of gad, TyrH and dbh

RGB tiff stack of average registered full-body gene expression patterns of neurotransmitter marker genes gad (red), TyrH (green) and dbh (blue), created by RNA whole mount in situ hybridization in 3-day-old Platynereis larvae. Note that signal present in spinning glands and chaetae of larvae is background, however, signal in the parapodia in the TyrH (green) channel represents real gene expression

Double in situ hybridization for r-opsin1 and hdc

RGB tiff stack of raw data from high-resolution apical scan of double RNA whole mount in situ hybridization with r-opsin1 (red) and hdc (green) in 3-day-old Platynereis larvae. Larva is counterstained with acetylated tubulin antibody (blue) to show cilia and axonal scaffold

High-resolution image stack of the Platynereis larval primary optic neuropil

High-resolution (1.13 nm/pixel) image stack (tiff series) of the Platynereis larval primary optic neuropil. Contains 4 layers of 22,304 x 5680 pixels