Molecular Detection of Leishmania spp. in Skin and Blood of Stray Dogs from Endemic Areas of Cutaneous Leishmaniasis in Saudi Arabia

Dogs can act as reservoirs of canine leishmaniasis, caused by Leishmania species. The aims of this study were to determine the prevalence of canine leishmaniasis using a PCR technique among stray dogs living in three provinces of Saudi Arabia, Riyadh, Al-Ahsa Oasis and Al-Qaseem, here the disease is endemic; and to identify and document different Leishmania to species levels Methods: This cross-sectional investigation was conducted, from Mar 2016 to Apr 2018, in three parts of Saudi Arabia: Central province (Riyadh), Eastern province (Al-Ahsa Oasis) and Al-Qaseem province. Blood samples were collected from 526 dogs; 40 presented cutaneous nodules so were suspected clinically of cutaneous leishmaniasis. Biopsy tissue collections and parasite cultures were performed. A generic kDNA was performed using different primers for Leishmania differentiation. Results: All blood samples were negative for Leishmania infantum infection by molecular analysis, though forty dogs had thick cutaneous lesions in different parts of their body. Four dogs’ skin lesions were associated with dermatitis, splenomegaly and lymphadenomegaly. Parasite culture was used to diagnose cutaneous leishmaniasis, identifying 31/40 (77.5%) positive samples. Overall, of 526 samples, the prevalence of L. major and L. tropica was found to be 4% and 1.9%, respectively. Gender and age had a significant effect on Leishmania prevalence: (P=0.0212 and0.0357), respectively. Conclusion: This was the first molecular study of dog leishmaniasis from Saudi Arabia of dogs confirmed to have cutaneous leishmaniasis. Further epidemiological and molecular investigations of domestic and wild canine infections with L. major, L. tropica and L. infantum in endemic and nonendemic areas of Saudi Arabia are required, for leishmaniasis control

Tick-Borne Pathogens in Ticks and Blood Samples Collected from Camels in Riyadh Province, Saudi Arabia

Background and Objective: Domestic animals, including camels, in Saudi Arabia suffer from various diseases, among which tick-borne infections are important because they reduce the productivity of these animals. However, knowledge of tick-borne pathogens in camels in Saudi Arabia is very limited, so the aims of this study were to quantify the abundance and distribution of tick species infesting camels from different districts of Riyadh province and use molecular tools to detect tick-borne pathogens in both the ticks and blood samples. Materials and Methods: A total of 218 ticks were collected from 116 camels from the 5 districts of Riyadh. The ticks and camel blood samples were analyzed for Borrelia, Babesia and Theileria pathogens using conventional and real-time PCR. Results: The results showed that five different tick species were identified. Majority of the ticks were Hyalomma dromedarii (70.6%), which were collected from camels in all 5 districts. This was followed by Hyalomma impeltatum species (25.2%), which was again found in all the districts. The other species found were Hyalomma anatolicum, Haemaphysalis sp. and Rhipicephalus turanicus. The only one H. dromedarii tick was positive for Theileria sp. DNA. Although the sample size and the area of tick collection were limited, the data suggest that the prevalence of pathogens in the Riyadh province, Saudi Arabia is relatively low. Conclusion: The study provides useful preliminary data to inform future full-scale country-wide surveys

Undifferentiated HepaRG cells show reduced sensitivity to the toxic effects of M8OI through a combination of CYP3A7-mediated oxidation and a reduced reliance on mitochondrial function

\ua9 2024 The AuthorsThe methylimidazolium ionic liquid M8OI (1-octyl-3-methylimidazolium chloride, also known as [C8mim]Cl) has been detected in the environment and may represent a hazard trigger for the autoimmune liver disease primary biliary cholangitis, based in part on studies using a rat liver progenitor cell. The effect of M8OI on an equivalent human liver progenitor (undifferentiated HepaRG cells; u-HepaRG) was therefore examined. u-HepaRG cells were less sensitive (>20-fold) to the toxic effects of M8OI. The relative insensitivity of u-HepaRG cells to M8OI was in part, associated with a detoxification by monooxygenation via CYP3A7 followed by further oxidation to a carboxylic acid. Expression of CYP3A7 - in contrast to the related adult hepatic CYP3A4 and CYP3A5 forms - was confirmed in u-HepaRG cells. However, blocking M8OI metabolism with ketoconazole only partly sensitized u-HepaRG cells. Despite similar proliferation rates, u-HepaRG cells consumed around 75% less oxygen than B-13 cells, reflective of reduced dependence on mitochondrial activity (Crabtree effect). Replacing glucose with galactose, resulted in an increase in u-HepaRG cell sensitivity to M8OI, near similar to that seen in B-13 cells. u-HepaRG cells therefore show reduced sensitivity to the toxic effects of M8OI through a combination of metabolic detoxification and their reduced reliance on mitochondrial function

Identification and characterization of antibacterial compound(s) of cockroaches (Periplaneta americana)

Infectious diseases remain a significant threat to human health, contributing to more than 17 million deaths, annually. With the worsening trends of drug resistance, there is a need for newer and more powerful antimicrobial agents. We hypothesized that animals living in polluted environments are potential source of antimicrobials. Under polluted milieus, organisms such as cockroaches encounter different types of microbes, including superbugs. Such creatures survive the onslaught of superbugs and are able to ward off disease by producing antimicrobial substances. Here, we characterized antibacterial properties in extracts of various body organs of cockroaches (Periplaneta americana) and showed potent antibacterial activity in crude brain extract against methicillin-resistant Staphylococcus aureus and neuropathogenic E. coli K1. The size-exclusion spin columns revealed that the active compound(s) are less than 10 kDa in molecular mass. Using cytotoxicity assays, it was observed that pre-treatment of bacteria with lysates inhibited bacteria-mediated host cell cytotoxicity. Using spectra obtained with LC-MS on Agilent 1290 infinity liquid chromatograph, coupled with an Agilent 6460 triple quadruple mass spectrometer, tissues lysates were analyzed. Among hundreds of compounds, only a few homologous compounds were identified that contained isoquinoline group, chromene derivatives, thiazine groups, imidazoles, pyrrole containing analogs, sulfonamides, furanones, flavanones, and known to possess broad-spectrum antimicrobial properties, and possess anti-inflammatory, anti-tumour, and analgesic properties. Further identification, characterization and functional studies using individual compounds can act as a breakthrough in developing novel therapeutics against various pathogens including superbugs

Influence of age on measured anatomical and physiological interpupillary distance (far and near), and near heterophoria, in Arab males

Saud A AlAnazi, Mana A AlAnazi, Uchechukwu L Osuagwu Corneal Research Chair, Department of Optometry and Vision Science, College of Applied Medical Sciences, King Saud University, Kingdom of Saudi Arabia Purpose: To compare the effects of age and near phoria on interpupillary distance measured at far (FIPD) and near (NIPD) using the Viktorin&#39;s and pupillometer (PD-5) methods. Methods: Interpupillary distance (IPD) by Viktorin&#39;s method and the corneal reflex pupillometer (PD-5) method, as well as near heterophoria by the Saladin near point card, were each obtained on 133 randomly selected normal subjects aged 20&ndash;67 years. Comparison within and between techniques, influence of age on IPD, and near heterophoria were assessed. Results: The mean FIPD varied significantly from the NIPD (P < 0.001 for both methods). Overall, FIPD ranged from 56&ndash;73 mm, and NIPD ranged from 50&ndash;70 mm. For FIPDs of 55 mm, 63 mm, and 71 mm, the corresponding NIPDs were less by 3.9 mm, 4.4 mm, and 5.0 mm, respectively, which were measured by Viktorin&#39;s method, and 4.0 mm, 4.6 mm, and 5.2 mm, respectively, measured by the PD-5 method. Between methods, the limits of agreement were: -3.9 mm and 3.2 mm (P > 0.05) for FIPD, and -3.1 mm and 2.9 mm (P > 0.05) for NIPD. Both IPDs varied significantly across age groups (P < 0.0001). Post hoc analysis revealed a significant variation (P < 0.01 in both techniques) only in the comparison between age groups 16-25 years and 41-67 years. The mean difference was -2.2 mm (-4.0 mm to -0.3 mm) and -2.8 mm (-4.7 mm to -1.0 mm) for Viktorin&#39;s method for FIPD and NIPD, respectively. For PD-5, the corresponding values were -1.3 mm (-3.2 mm to 0.4 mm) and -1.7 mm (-4.1 to -0.5 mm). Also, the near phoria differed significantly (P < 0.0001) across age groups and correlated positively with age (r2 = 0.27, P < 0.0001) and NIPD (r2 > 0.04; P < 0.03, both techniques). Conclusion: The difference between FIPD and NIPD (about 4.4 mm in Arab males) was observed independent of the technique used. The Viktorin&#39;s and the PD-5 methods of IPD assessment resulted in similar values, and therefore, could be interchangeably used. However, caution is advised in cases of high power refractive corrections as the difference could vary from -4 mm to +3 mm (FIPD) and -3 mm to +3 mm (NIPD). Both IPDs have demonstrated an increase until the patients are in their early 40s, and a slight decrease has been observed thereafter. Age and NIPD were significantly associated with heterophoria in our subjects. Keywords: phoria, interpupillary distance, Viktorin&#39;s method, pupillometer, Saladin near point card, ag

Predictors of Leadership Competencies Among Nurse Executives in the Kingdom of Saudi Arabia

Yousef A Alshamlani,1,&ast; Naif H Alanazi,2,&ast; Sami A Alhamidi,2,&ast; Raeed A Alanazi,2,&ast; Atallah Alenezi,3,&ast; Norisk M Adalin,1,&ast; Seham M Alyousef,2,&ast; Regie B Tumala2,&ast; 1Nursing Department, King Saud University Medical City, Diriyah, Riyadh, Kingdom of Saudi Arabia; 2College of Nursing, King Saud University, Diriyah, Riyadh, Kingdom of Saudi Arabia; 3Nursing Department, College of Applied Medical Sciences, Shaqra University, Shaqra, Kingdom of Saudi Arabia&ast;These authors contributed equally to this workCorrespondence: Regie B Tumala, College of Nursing, King Saud University, Diriyah, Riyadh, 11421, Kingdom of Saudi Arabia, Tel +966554773976, Email [email protected]: Awareness and development of nurse executives’ leadership competencies has been considered a key strategy for the success of healthcare organizations. However, only a few studies about nurse executives’ leadership competencies are available in the literature, and no study has been conducted about the topic within Saudi Arabian context. The aim of this study was to assess the leadership competencies of nurse executives and the predictors of these competencies.Methods: This quantitative study used a descriptive, cross-sectional, and correlational design. The study recruited a total convenience sample of 136 nurse executives who voluntarily consented to participate. The respondents self-rated the online survey, “Leadership Competency Assessment Tool: Self-Assessment.” Data collection was conducted between September 2022 and November 2022. Test of correlation and multiple regression analyses were conducted.Results: The overall mean score of nurse executives’ leadership competencies was 3.38 out of 5.0, which was below the level of having excellent leadership competencies. Four dimensions had mean scores below the level of having excellent leadership competencies, namely, communication and relationship building (3.48), knowledge of the healthcare environment (3.29), leadership (3.45), and business skills (3.15), while nurse executives had excellent leadership competencies only in the professionalism dimension (3.51). Age (p=0.04), highest educational attainment (p=0.01), current role (p< 0.01) and length of experience (p=0.02) as nurse executive, monthly salary (p=0.03), nationality (p=0.04), and having seminars/trainings in leadership and management (p=0.01) were considered predictors of the leadership competencies of nurse executives.Conclusion: Nurse executives reported a low level of leadership competencies, thus implying necessary actions for improvement. There were personal factors that influenced the level of nurse executives’ leadership competencies. To achieve quality outcomes and maintain a high level of nursing care in hospital organizations, these findings must be considered in planning strategies related to the improvement of the leadership competencies of nurse executives.Keywords: assessment, leadership competencies, nurse executives, predictors, Saudi Arabi

Assessment of the tear film in normal eye subjects after consumption of a single dose of hot peppermint drink

Ali M Masmali, Saud A Alanazi, Bader Almagren, Gamal A El-HitiCornea Research Chair, Department of Optometry, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Saudi ArabiaObjective: To test the effect of a single dose of hot peppermint consumption on the tear film in normal eye subjects.Methods: Thirty healthy male subjects aged 18&ndash;39 years (23.20&plusmn;2.17 years) were enrolled. Also, an age-matched control group of 30 male subjects (19&ndash;39 years, 23.50&plusmn;0.70 years) was enrolled to test the effect of the hot water. Tear meniscus height (TMH), noninvasive tear breakup time (NITBUT), and tear ferning (TF) tests were performed for each patient 30 mins before and 60 mins after they drank hot peppermint.Results: Mean TMH measurements were higher postintervention (0.32&plusmn;0.07) than preintervention (0.27&plusmn;0.04 mm). Similarly, mean TF grades were significantly higher (2.07&plusmn;1.20) postintervention than preintervention (0.84&plusmn;0.71). By contrast, mean NITBUT was lower postintervention than preintervention (11.57&plusmn;3.17 and 15.84&plusmn;3.36, respectively). TMH measurements increased in 90% of the subjects. Conversely, NITBUT decreased in 96.7% of the subjects. For the control group that tests the effect of drinking hot water, the scores did not differ significantly in the three tests pre- and postintervention (P˂0.05). TF grades increased in 93.3% of the subjects, postintervention.Conclusion: Tear film quality decreases significantly after peppermint beverage consumption. A similar observation has been made on the consumption of hot green tea drink.Keywords: peppermint drink, tear film, tear ferning test, phenol red thread test, eye dryness, polyphenol

The acute effect of a single dose of green tea on the quality and quantity of tears in normal eye subjects

Ali M Masmali, Saud A Alanazi, Abdullah G Alotaibi, Raied Fagehi, Ali Abusharaha, Gamal A El-Hiti Cornea Research Chair, Department of Optometry, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Saudi Arabia Objective: The study aimed to investigate the acute effect of a single dose of green tea on the quality and quantity of tears in normal eye subjects. Methods: Forty normal eye subjects (22 men and 18 women) aged 19&ndash;39&nbsp;years were enrolled in the study. Also, an age matching control group (20 males and 20 females) was enrolled for comparison. McMonnies dry eye symptoms questionnaire and slit lamp were used for the exclusion criteria determination. Phenol red thread (PRT) test was performed on both eyes of each subject. A tear sample was collected from the lower tear meniscus of the right eye of each subject for the tear ferning (TF) test, which was performed before (30&nbsp;minutes) and after (60&nbsp;minutes) drinking green tea. Results: The median PRT measurement after green tea consumption was lower (median [IQR]=23.50 [8.00] mm) compared to that before consumption (median [IQR]=27.00 [8.75]&nbsp;mm). In contrast, the median TF grade was significantly higher following green tea consumption than that before consumption (median [IQR]=2.65 [1.23] vs 1.50 [0.88] mm, respectively). The results show that TF grades increased in 97.5%, and the red phenol thread scores decreased in 80% of the subjects after green tea consumption compared to those before consumption. The PRT readings and TF grades were significantly different (P&lt;0.05) before and after green tea consumption. Conclusion: Drinking green tea could have a significant effect on the eye tear film quality. Tear quality appeared to decrease after green tea consumption. Keywords: dry eye, tear ferning test, green tea, phenol red thread test, tear film, polyphenol