Genotypic and phenotypic characterization of enteric bacteria in an integrated population of swine and humans

In two longitudinal studies, we examined the transmission dynamics of antimicrobial resistance (AMR) in an integrated, semi-closed population of humans and swine

Longitudinal Study of Antimicrobial Resistance among Escherichia coli Isolates from Integrated Multisite Cohorts of Humans and Swine

In a 3-year longitudinal study, we examined the relationship between the seasonal prevalence of antimicrobial-resistant (AR) Escherichia coli isolates from human wastewater and swine fecal samples and the following risk factors: the host species, the production type (swine), the vocation (human swine workers, non-swine workers, and slaughter plant workers), and the season, in a multisite, vertically integrated swine and human population representative of a closed agri-food system. Human and swine E. coli (n = 4,048 and 3,429, respectively) isolates from wastewater and fecal samples were tested for antimicrobial susceptibility, using the Sensititre broth microdilution system. There were significant (P < 0.05) differences among AR E. coli prevalence levels of (i) the host species, in which swine isolates were at higher risk for resistance to tetracycline, kanamycin, ceftiofur, gentamicin, streptomycin, chloramphenicol, sulfisoxazole, and ampicillin; (ii) the swine production group, in which purchased boars, nursery piglets, and breeding boars isolates had a higher risk of resistance to streptomycin and tetracycline; and iii) the vocation cohorts, in which swine worker cohort isolates exhibited lower sulfisoxazole and cefoxitin prevalence than the non-swine worker cohorts, while the slaughter plant worker cohort isolates exhibited elevated cefoxitin prevalence compared to that of non-swine workers. While a high variability was observed among seasonal samples over the 3-year period, no significant temporal trends were apparent. There were significant differences in the prevalence levels of multidrug-resistant isolates between host species, with swine at a higher risk of carrying multidrug-resistant strains than humans. Considering vocation, slaughter plant workers were at higher risk of exhibiting multidrug-resistant E. coli than non-swine workers

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Prevalence and antimicrobial susceptibility pattern of methicillin-resistant Staphylococcus aureus isolates from retail raw meats in Turkey

The transmission of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) through food products of animal origin may pose a public health concern. Therefore, the objectives of the present work were to determine the prevalence of S. aureus and MRSA in raw meat samples (beef, chicken, turkey, and duck) at retail level, and to determine the antimicrobial resistance profile of the MRSA isolates. Between September 2018 and January 2019, a total of 325 raw meat samples were collected from retail stores, and analysed for the presence of S. aureus and MRSA using primary enrichment method as well as using secondary selective enrichment methods for MRSA detection. All the suspected S. aureus and MRSA colonies obtained from the samples were confirmed by both phenotypic and genotypic methods. The MRSA isolates were tested against various antimicrobials, and the minimal inhibitory concentration (MIC) of vancomycin was determined. Macrolide-lincosamide-streptogramin B (MLSB) resistance phenotypes were also screened using the double-disk diffusion test. The overall prevalence of S. aureus was 57.2%, whereas the food-specific prevalence in beef, turkey, duck, and chicken samples was 75, 53, 48, and 46%, respectively. The overall prevalence of MRSA was 1.2%, whereas it was 3, 1, 0, and 0% in beef, turkey, chicken, and duck samples, respectively. All MRSA isolates were detected by the secondary selective enrichment method. These MRSA isolates had a variety of MLSB resistance phenotypes (i.e., iMLSB, cMLSB, and MS-MLSB) with vancomycin MIC values ranging between 1 -2 mu g/mL. These findings are important for developing interventions to reduce raw meat contamination with S. aureus and MRSA, and to improve public health. (c) All Rights Reserve

Vancomycin-Resistant Enterococcus faecium Strains Isolated from Community Wastewater from a Semiclosed Agri-Food System in Texas

Vancomycin-resistant Enterococcus faecium strains (VRE) were isolated from human wastewater but not swine fecal waste from a semiclosed agri-food system in Texas. Forty-nine VRE isolates possessed vanA, and one possessed vanB. Twenty-one pulsed-field gel electrophoresis types were identified and segregated into three groups. There was evidence of clonal dissemination among geographically separated sites

Permanganate oxidation of 1,5,9-trienes: stereoselective synthesis of tetrahydrofuran-containing fragments

Permanganate oxidation of farnesoate esters 12a-d afforded perhydro-2,2'-bifuranyl compounds 16a-d, with control of relative stereochemistry at four new stereocenters. Subsequent oxidative cleavage of 16a-d then provided tetrahydrofuran-containing fragments 17a-d, one of them 17b possessing the same relative stereochemistry present in the C13-C21 portion of the polyether antibiotic semduramycin (1). Control of the absolute stereochemistry was achieved through the use of the Oppolzer sultam chiral auxiliary. The requisite starting trienes were prepared stereoselectively in just three steps from geranyl chloride or neryl chloride, providing a short and versatile route to polyether fragments