In vitro antioxidant activities of resveratrol, cinnamaldehyde and their synergistic effect against cyadox-induced cytotoxicity in rabbit erythrocytes

This study was conducted to explore the potential benefits of using cinnamaldehyde (CIN), resveratrol (RES) separately or in combination on cyadox (CYA)-induced alterations in isolated rabbit erythrocytes. Erythrocytes suspensions were partitioned into 7 groups (5 replicates/group), 1st kept as control treated with phosphate buffered saline (PBS) with dimethyl sulphoxide (DMSO); 2nd group was subjected to CYA (40 μg/ml), 3rd group was incubated with CIN (40 μM), 4th group was subjected to RES (40 μM), 5th group was co-exposed to CYA (40 μg/ml) and CIN (40 μM), 6th group was co exposed to CYA (40 μg/ml) and RES (40 μM), and 7th group was exposed to CYA in combination with both CIN and RES at the same indicated concentrations. The reaction mixtures of different groups were incubated at 37 °C for 3 h with gentle shaking every 15 minutes. Our results revealed that exposure to CYA caused a significant decrease (linear and quadratic) in superoxide dismutase (SOD) and catalase (CAT) activities and the contents of reduced glutathione (GSH) and glutathione transferase (GST). Incubation of erythrocytes with CYA increased GSSG content, GSSG/GSH ratio, malonaldehyde (MDA) and protein carbonyl (PrC) concentrations while it decreased the total protein (TP). CYA also lead to hemolysis and energy depletion of erythrocytes beside activation of caspase cascades, suggesting the pro-oxidant effect CYA that could be implicated in eryptosis. CIN and RES were able to inverse these hazardous effects of CYA. However, CIN was more effective than RES, their combination showed a positive synergistic effect in protecting the cells against oxidative injury caused by CYA

Age and sex-related differences in performance, carcass traits, hemato–biochemical parameters, and meat quality in Japanese quails

ABSTRACT The effect of sex and age of Japanese quails (Coturnix japonica) on their performance, blood biochemical parameters, carcass traits, and meat quality was evaluated in this experiment. A total of 450, 3-wk-old Japanese quails (225 males and 225 females) were equally divided into six groups (75 birds each) in a 2 × 3 factorial design, including two sex (male and female) and three slaughter ages (5, 6, and 7 wk of age). Each group was subdivided into five replicates each of 15 birds. The body weight (BW) and feed intake (FI) values were significantly higher in female quails than in males, and these values showed an increasing trend with age. Female quails recorded the higher percentages of liver and giblets, and lower heart percentage than males. Sex × age interaction had a significant impact on carcass parameters except for breast and thigh yield. No significant sex-based differences were detected for quail meat chemical composition except for meat fat content. Moisture and ash content of meat were gradually deceased with age. Meat from female showed higher values for tenderness, intramuscular fat percentage (IMF), and meat lightness, but showed lower values of water holding capacity (WHC) and meat redness. Meat juiciness and tenderness decreased, while WHC, IMF, and meat redness increased with age. Mean values of red blood cells (RBC), packed cell volume (PCV), hemoglobin (Hb), and white blood cells (WBC) were higher (

Rice Bran as an Alternative Feedstuff in Broiler Nutrition and Impact of Liposorb® and Vitamin E-Se on Sustainability of Performance, Carcass Traits, Blood Biochemistry, and Antioxidant Indices

The impact of dietary rice bran with or without feed additives on the performance, carcasses, and blood profiles of chickens was examined. A total of 245 unsexed one-week-old broiler chicks were divided into seven groups, with seven replications of five chicks each. The treatments were: (1) control, (2) 5% rice bran, (3) 5% rice bran + 0.5 g/kg of Liposorb®, (4) 5% rice bran + 1 g/kg of vitamin E-Se, (5) 10% rice bran, (6) 10% rice bran + 0.5 g/kg Liposorb®, and (7) 10% rice bran + 1 g/kg of vitamin E–selenium. Considering the entire experimental period, it did not affect the in vivo performance of the broilers. However, all the experimental diets decreased dressing % compared with the control (p < 0.01) and the worst values were obtained for the 10% RB groups (75.7, 75.9, and 75.8%, respectively, for 10%RB, 10%RB + Liposorb, and 10%RB + Vit. E-Se groups). All the experimental diets decreased (p < 0.01) the albumin/globulin ratio due to an increased level of serum globulins. Differences in lipid profiles, antioxidants, and immunity parameters in plasma were not related to dietary treatments. In conclusion, the use of rice bran up to 10% in diets had no harmful effect on the overall growth performance of the broilers from 1 to 5 weeks of age. Still, carcass characteristics were negatively affected, except for heart percentage. In addition, the supplementation of Liposorb® or vitamin E-Se to rice bran diets did not recover these harmful effects. Thus, rice bran could be utilized at 10% in broiler diets when growth performance was considered; further research is required

Marburg Virus Disease – A Mini-Review

Marburg virus disease (MVD) is a highly fatal disease caused by the Marburg virus (MARV) which belongs to the family Filoviridae. The disease has been recently reported from Ghana, an African country, and nearly 15 outbreaks of MVD have been reported in the past five decades. Various species of bats viz., Rousettus aegyptiacus, Hipposideros caffer, and certain Chiroptera act as the natural source of infection. Pathophysiology of the disease reveals severe antiviral suppression due to changes in gene expression and interferon-stimulated gene (ISG) production in the hepatic cells. With the progression of the disease, there may be the development of pain in the abdomen, nausea, vomition, pharyngitis, and diarrhea along with the onset of hemorrhagic manifestations which may lead to the death of a patient. The advent of molecular detection techniques and kits viz., reverse transcription polymerase chain reaction (RT-PCR) kit has greatly aided in the diagnosis of MVD. Identification of the virus in the specimen with great accuracy can be done by whole viral genome sequencing. The use of a combination of MR-186-YTE (monoclonal antibody) and an antiviral drug named remdesivir in the NHP model is greatly effective for eliminating MARV. The protective effect of a Vesicular stomatitis virus (VSV) (recombinant) - based vaccine expressing the glycoprotein of MARV has been revealed through animal model studies, other vaccines are also being developed. Proper health education, personal hygiene and precautions by health care workers while handling patients, good laboratory facilities and service along with the establishment of enhanced surveillance systems are the need of the hour to tackle this highly fatal disease. This article presents an overview of different aspects and salient features of MARV / MVD, and prevention and control strategies to be adopted

Dietary supplementation of quercetin nanoparticles enhances the growth performance hematological and immunological responses and resistance to Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus) exposed to silver nanoparticles toxicity

The wide incorporation of silver nanoparticles (AgNPs) in aquaculture could result in releasing of these particles into the environment with environmental impacts and health hazards. Thus, this study aimed to investigate the impacts of the dietary supplementation of quercetin nanoparticles (QNPs) on Nile tilapia (Oreochromis niloticus) growth, antioxidants, and immunity besides its susceptibility to the infection with Aeromonas hydrophila with or without the aqueous exposure to AgNPs. First, the 96-h LC50 value for AgNPs in O. niloticus wa estimated to be 19.81 mg/L (the 1/10th of the 96 h LC50 of AgNPs = 1.98 mg/L). Then a total of 240 Nile tilapias (40.00 ± 0.45 g) were randomly distributed into 4 groups (each group contains 60 fish in 3 replicates of 20 fish). The 1st group (control) was fed on a basal diet without QNPs and AgNPs. The 2nd group (QNPs) received the basal diet supplemented with QNPs (400 mg/kg), the 3rd group (AgNPs) was exposed to 1/10th 96-h LC50 of AgNPs (1.98 mg/L) and fed on QNPs free diet, while the 4th group (AgNPs+ QNPs) was exposed to AgNPs (1.98 mg/L) and cosupplemented with QNPs (400 mg/kg). Besides, AgNPs resulted in marked elevation in the serum myeloperoxidase (MPO) 8-hydroxy-2-deoxyguanosine (8-OHdG) contents, and declines in lysozyme activity and the levels of nitric oxide (NO) and immunoglobulins (IgM and IgG) in the exposed fish (AgNPs group). These effects were accompanied with significant downregulation in the relative mRNA expressions of SOD, CAT, and GSH and upregulation in the expressions of INF-γ, TNF-α, and Il-1β. Feeding QNPs-enriched diet, alone or combined with AgNPs exposure, conversed most of these effects and restored some to the control levels. AgNPs exposure increased mortalities, lowered survival rates and altered Nile tilapia’s resistance to A. hydrophila infection in the AgNPs exposed fish, whereas co-supplementation with QNPs enhanced their resistance with less mortalities. In summary, the reported immunomodulation, and protective properties of QNPs dietary supplementation, strengthen its applicability as an effective and promising feed supplement to alleviate the AgNPs associated toxicity in fish

Evaluation of genetic diversity within different rabbit (Oryctolagus cuniculus) genotypes utilizing start codon targeted (SCoT) and inter-simple sequence repeat (ISSR) molecular markers

This work aimed at studying the genetic diversity among different rabbit genotypes reared in Egypt by two different molecular markers (start codon targeted, SCoT, and inter-simple sequence repeat, ISSR) to improve breeding strategies. Five different groups of rabbits were used Gabali (Gab), New Zealand white (NZW), Californian (Cal), Rex, and Papillon (Pap). DNA was extracted and analyzed using SCoT and ISSR-PCR, and the obtained fragments were analyzed. Six SCoT primers scored 60 bands with 78.33 % polymorphism; primer SCoT 6 was the most polymorphic marker with 92.31 % polymorphism, while SCoT 5 was the lowest with 60 %. A dendrogram based on SCoT-PCR revealed that the Rex breed was the most genetically different. Seven ISSR primers gained 56 bands in total with 49.762 % polymorphism. ISSR 4 was the most polymorphic primer that detected 75 % of polymorphism, while ISSR 6 was not able to detect any polymorphism. It was suggested that the SCoT markers may be more effective than ISSR for differentiating and identifying the genetic variations within investigated breeds. Also, the usage of molecular markers of SCoT and ISSR may be more proper for calculating genetic diversity and common ancestry among tested rabbit breeds. Furthermore, evaluating genetic variability is important for enhancing existing breeds' adaptation to ecological alterations and crucial for preservation or breeding purposes

Dietary Supplementation of Nile Tilapia (Oreochromis niloticus) With Panax ginseng Essential Oil: Positive Impact on Animal Health and Productive Performance, and Mitigating Effects on Atrazine-Induced Toxicity

The present study assessed the impact of Panax ginseng essential oil (GEO) supplementation on physiological parameters related to productive performance and health status in Nile tilapia reared under standard conditions and exposed to a sub-lethal atrazine (ATZ) concentration. Fish were allocated into 6 groups: the control group was reared in clean water and fed with a commercial basal diet (CNT), two groups were fed with the basal diet supplemented with two different levels of GEO (GEO1 and GEO2, respectively), one group was intoxicated with 1/5 of ATZ 96-h lethal concentration 50 (1.39 mg/L) (ATZ group), and the remaining two groups were fed with the GEO-supplemented diets and concurrently exposed to 1.39 mg ATZ/L (GEO1+ATZ and GEO2+ATZ, respectively). The experiment lasted for 60 days. GEO supplementation exerted a significantly positive influence on fish growth, feed utilization, and hepatic antioxidant defense systems at both levels of supplementation. ATZ exposure significantly reduced fish survival rates and impaired fish growth and feed utilization, with the lowest final weights, weight gain, total feed intake, and the highest feed conversion ratio being recorded in the ATZ-intoxicated group. ATZ exposure caused significant changes in intestinal digestive enzyme activity (decreased lipase activity), hematological indices (decreased hemoglobin, packed cell volume, erythrocytes, and leukocytes), blood biochemical variables (decreased total proteins, albumin, globulins, and immunoglobulin M; increased total cholesterol, triglycerides, and cortisol), and hepatic oxidative/antioxidant indices (decreased glutathione level, superoxide dismutase and catalase enzyme activity and mRNA expression levels, and increased malondialdehyde content). Moreover, in the hepatic tissue of ATZ-intoxicated Nile tilapia, histopathological alterations and upregulated mRNA expression levels of stress- and apoptosis-related genes (Hsp70, caspase 3, and p53) were observed. GEO supplementation in ATZ-treated groups significantly attenuated the aforementioned negative effects, though some parameters did not reach the CNT values. These findings provide further and partly new evidence that sub-lethal ATZ toxicity induces reduced survivability, growth retardation, impaired digestive function, anemia, immunosuppression, hepatic oxidative stress damage, and overall increased stress level in Nile tilapia, and suggest that GEO supplementation may be useful for mitigating this toxicity and provide more general support to the productive performance and health status of this fish species

Leverage of Matricaria chamomilla L. Oil Supplementation over Ochratoxin A in Growing Quails

Ochratoxin A (OTA) is one of the mycotoxins in the agriculture and livestock sectors. The poultry sector su3ered from significant economic losses due to the adverse impacts of OTA on the growth rate, feed conversion ratio, and livability. Thus, the present investigation aimed to determine the impact of chamomile essential oil supplementation against OTA toxicity in growing quails. 360 one-week-old growing quails were distributed into six groups (n = 60) with four replicates of 15 birds. The groups were G1 (control negative), G2 (OTA 1 mg/kg diet, control positive), G3 (chamomile oil 0.5 g/kg diet), G4 (chamomile oil 1 g/kg diet), G5 (OTA 1 mg/kg diet + chamomile oil 0.5 g/kg diet), and G6 (OTA 1 mg/kg diet + chamomile oil 1 g/kg diet). Adding OTA significantly (P &lt; 0.05) reduced live body weight and weight gain at 5 weeks. Feed intake at 5 weeks was non significantly reduced in G3 and G4 compared to G1. G4 showed a significant (P &lt; 0.05) increase in weight gain and the lowest feed conversion ratio. The G2 showed the lowest superoxide dismutase (SOD), total antioxidant capacity (TAC), glutathione transferase (GST) activity, and the highest levels of malondialdehyde (MDA). Moreover, they showed a significant improvement in liver enzymes and kidney function tests and a significant (P &lt; 0.05) reduction in the levels of total cholesterol and triglycerides. Chamomile supplementation alone or with OTA significantly (P &lt; 0.05) increased immunoglobulin M, G, A, and complement 3 than OTA alone. Chamomile oil with an OTA diet or alone reduced the negative effects of OTA and improved the performance, antioxidant status, lipid profile, and immunological state of growing Japanese quails

Effect of Quercetin Nanoparticles on Hepatic and Intestinal Enzymes and Stress-Related Genes in Nile Tilapia Fish Exposed to Silver Nanoparticles

The current study investigated the potential mitigative effect of quercetin nanoparticles (QNPs) against silver nanoparticle-induced toxicity in Nile tilapia. Fish were grouped into the control, fish exposed to 1.98 mg L–1 silver nanoparticle (AgNPs), fish that received 400 mg L–1 24 QNPs, and fish that received both QNPs and AgNPs at the same concentrations for 60 days. The moisture and ash contents of the AgNP group were significantly higher than those of the other groups. In contrast, the crude lipid and protein were decreased in the whole body. AgNPs significantly increased serum levels of ALT, AST, total cholesterol, and triglycerides and decreased glycogen and growth hormone. The activities of antioxidants were significantly inhibited, and the levels of oxidative damage were increased in the liver of the AgNP group. AgNPs upregulated the expression of hepatic HSP70, caspase3, and p53 genes. AgNPs inhibited intestinal enzyme activities, increased intestinal bacterial, and Aeromonas counts, and increased Ag residues in the liver. These findings indicate the oxidative and hepatoxic effects of AgNPs. QNPs enhanced and restored physiological parameters and health status under normal conditions and after exposure to AgNPs

Immunosuppressive Effects of Thallium Toxicity in Nile Tilapia Fingerlings: Elucidating the Rescue Role of Astragalus membranaceus Polysaccharides

This study evaluated the immunotoxic effects of thallium (Tl) in Nile tilapia fingerlings and the recovery role of dietary Astragalus membranaceus polysaccharides (ASs). An 8-week experiment was designed where 180 fishes were randomly and equally assigned in triplicates into the six groups: the control group (CNT) was reared in unpolluted water and fed a commercial diet, two groups were fed a well-balanced commercial diet plus 1.5 and 3.0 g AS/kg diet (AS0.15 and AS0.30), respectively, the fourth group was exposed to a sublethal dose of Tl (41.9 μg l−1) [equal to 1/10 of 96-h lethal concentration 50 (LC50)], and the last two groups were fed 0.15 and 0.3% AS, respectively, and concurrently exposed to Tl (41.9 μg l−1) (AS0.15+Tl and AS0.30+Tl). Fish hematobiochemical parameters, serum immunity [nitric oxide, total immunoglobulin M (IgM) levels, and lysozyme activity], transcription of hepatic interferon-g (IFN-g), interleukin-1b (IL-1b), and tumor necrosis factor-a (TNF-a), and resistance to Aeromonas hydrophila (A. hydrophila) were assessed. Hematobiochemical parameters and serum immune indices were significantly decreased in the fish group exposed to sublethal Tl concentration compared to the CNT group. Furthermore, Tl exposure significantly induced overexpression of IL-1b, TNF-a, and IFN-g genes (4.22-, 5.45-, and 4.57-fold higher, respectively) compared to CNT values. Tl exposure also increased the cumulative mortality (%) in Nile tilapia challenged with A. hydrophila. Remarkably, the groups fed AS0.15+Tl and AS0.30+Tl significantly ameliorated all the aforementioned parameters, but did not reach CNT values. Our findings suggest the possible immunomodulating roles of dietary AS in recovering the immunotoxic effects of Tl in Nile tilapia. We can conclude that dietary AS would be useful for maintaining the immunity of Nile tilapia fingerlings