The Predictive Value of Vanin-1 in Pediatric Immune Thrombocytopenia

Background: Immune thrombocytopenia (ITP) has oxidative stress-related pathway as one of its possible mechanisms. Vanin-1 (VNN1) is an oxidative stress sensitive sensor. Objective: The present study was conducted to assess the clinical significance of VNN1 in pediatric ITP patients. Patients and Methods: A total of 50 kids with ITP have been involved in this work; 25 newly diagnosed, 25 chronic patients with ITP more than 12 months (15 responders and 10 non responders to treatment) and 25 apparently healthy children. All groups had their serum VNN-1 levels checked using a two-antibody sandwich enzyme-linked immunosorbent test. Results: The serum VNN1 level was significantly higher in ITP group than control group (p˂0.001). The level of serum VNN1 was significantly very high in newly diagnosed ITP group (p˂0.001) in comparison to chronic responders and chronic non responders ITP groups. Moreover, there was a significant increase in serum VNN1 level in chronic non responders ITP group (p˂0.001) compared to chronic responders group. In newly diagnosed ITP group, both age (r=-0.62, p=0.006) and the platelet count (r=-0.46, p=0.01) had a significant negative connection with serum VNN1 level. A cut-off value lower than 0.6 ng/ml for serum VNN1 could be used to distinguish the chronic non-responders ITP from chronic responders patients with 100 percent specificity and 90 percent sensitivity. Conclusion: From our current results, it is hypothesized that high VNN1 level plays a role in the pathogenesis and progress of pediatric ITP

Comparative study on the using of PEG and PAM as curing agents for self-curing concrete

There are many factors, which may affect on concrete quality. One of those is concrete curing. Self-curing concrete is the solution. It may produce by using chemical curing agents. The concept of those agents is to reduce the water evaporation from concrete. This research aims to study the effect of chemical curing agents on the behavior of self-curing concrete. Two different chemical curing agents were used to study the main mechanical properties of concrete. The main variables are; the type of curing agent (Polyethylene glycol "PEG400"–Poly Acrylamide "PAM") and its dosages. The results obtained in terms of compressive, tensile and flexure strength values. Test results showed that the self-curing concrete cured by each agent performed better in hardened properties compared to none cured concrete. Also, curing using the both agents together perform better than using each one individually

Biofunctional molecules from Citrullus colocynthis: An HPLC/MS analysis in correlation to antimicrobial and anticancer activities

Background: Citrullus colocynthis belongs to Family Cucurbitaceae. It grows widely in Egypt and Sudan and it has been used in folk medicine of Sudan and many other African countries as anti inflammatory, anti diabetic, and antioxidant agent. Objectives: To evaluate the antibacterial, antifungal, antiviral, anticancer activities of ethanolic crude extracts of the fruits, leaves, seeds and roots of this plant, as well as identifying them HPLC/MS. Materials and Methods: Dried fruits, seeds, leaves and roots of C. colocynthis were powdered and passed through a 40- mesh, then, the powders were extracted with 95% ethanol in a soxhlet apparatus. The residues were dried under reduced pressure in rotary evaporator. Crude extract from different plant parts were evaluated biologically and phytochemically. Results: All extracts showed good antifungal activities with inhibition zone ranges between 15.1 ± 0.32 to 25.6 ± 0.16 mm. In terms of plant organ, fruits were the most active. In term of fungal strain Aspergillus fumigatus and Geotricum candidum were the most sensitive. Against tested Gram +ve, all extracts showed good activities except roots, while antibacterial activity against Gram –ve showed that the fruits extract have good activity as it was the sole extract with activity against Pseudomonas aeruginosa. Test for antiviral activities showed moderate to weak inhibitions of cytopathic effect (CPE). Anticancer activities of different crude extracts showed that fruits had significant antitumor activities against all tested cell lines, the IC50 values were 24.6, 16, 18.5 and 19.7 µg /ml for HCT-116, MCF-7, Hep-G2 and Caco-2 respectively. Seeds extract was only active on HCT-116 and Hep G2 with IC50 =21.2 µg/ml for HCT-116 and 22.4 µg/ml for Hep G2. Leaves extract was only active against Hep G2 cancer cell line with IC50 19.7 µg/ml. Roots extract show weak antitumor activity on tested cell lines (IC50 values > 30µg/ml). HPLC/MS qualitative and quantitative analysis of different organs extracts revealed the presence of 21 compounds identified as fourteen cucurbitacins, three flavonoids, three tannins, and one sterol. The presence of cucurbitacins can explain most of the biological activities. Conclusion: The biological activities of colocynth different parts are due to the presence of secondary metabolites mainly cucurbitacins in addition to flavonoids and tannins.  These activities prove the use of this plant in folk medicine and deserve much more future exploration targeting their discovery in unexplored sources and their derivatives for improving their anticancer and antimicrobial abilities. Keywords: Citrullus colocynthis, crude extracts, antifungal, antibacterial, antiviral, anticancer, HPLC/M

Application of mobile cloud computing in emergency health care

Mobile applications in emergency health care help maintain patient confidentiality and manage patient records, data storage. Compiles and analyzes care of better quality care. new implementations come with new goals and technologies like using mobile application with cloud computing system and reducing the responding time to safe the patient life and give the patient best health care professional service transition to using of mobile application in emergency healthcare, this paper will present (MCCEH) mobile cloud computing in emergency health care model, mainly reducing the wasting time in emergency health care, The process starting once the accident occurred and the patient run the application, mobile application will detect the patient location and allow him to book nearest medical center or specialist in some emergency cases once the patient did the booking will send help request to medical center this process will include an online pre-register patient in the medical center to save time of patient registration, MCCEH model allows the patients to review the previous feedback and experiences of each specialist or medical center and allows doctors to be able to stay in contact with their patients more often and by communication through mobiles applications and share messages and photos of the accident or emergency case itself

Ureterorectostomy as a continent urinary diversion for complicated bladder exstrophy in children by using a modified Duhamel procedure: A case series

AbstractIntroductionWhatever the method and timing of surgery, a high proportion of children with bladder exstrophy will continue to suffer from urinary incontinence. They face the options of urinary diversion to an external stoma or construction of a neobladder from bowel. This study describes a modified Duhamel's rectal pouch with a ureterorectostomy was carried out on 11 children who had a failed repair of bladder exstrophy.Materials and methodsTen boys and one girl, aged from 4 to 7years (mean 5.5), had several unsuccessful operations for bladder exstrophy. All selected to have good renal function and no other anomalies, but were incontinent of urine and had a small contracted or prolapsed bladder. They underwent urinary diversion to the rectum using the Duhamel pullthrough technique, where the sigmoid colon was opened into the back of the anal canal above the dentate line, creating a rectal bladder and making use of the anal sphincter to control urine and stool. All were followed up for 24months (18–27months).ResultsIn this selected group of patients there were no major operative or postoperative complications. Follow-up for 2years revealed no deterioration in renal function, or electrolytes disturbance. They can hold up to 300ml of urine and all patients are continent during the daytime with an emptying frequency of 3–5 times. Nocturnal wetting occur some 4–8 times per month with significant decrease with time. Two cases developed pyelonephritis but this was controlled with medical treatment.ConclusionEleven children achieved effective urinary continence by ureteric diversion to the rectum using a modified Duhamel pullthrough technique. Two years follow up showed no complications, except bed wetting, but long term assessment is warranted

RT-PCR ANALYSIS OF GENES EXPRESSION TO EVALUATE THE BIOMEDICAL IMPORTANCE OF MEDICAL-HERBAL EXTRACTS IN DIABETES TREATMENT

Fenugreek is an annual crop belonging to the legume family grown in most parts of the world. It is known to possess a number of medicinal properties, but a little phytochemical research has been carried out on the seeds of species; Trigonella hamosa Leguminosae. Previously we reported that Trigonella seeds extract are shown to have hypoglycemic effects on experimental diabetic rats. The aim of the current study is to understand the molecular mechanism of antidiabetic actions of Trigonella. Therefore, we examined the expressions levels of genes involved in glucose metabolism in liver of diabetic rats. Upon this basis, streptozotocin (STZ)-diabetic rats are used to assess hepatic glucokinase (GCK), insulin like growth factor-1 (IGF- 1), and glucose transporters (GLUT-2) genes expressions after Trigonella administration by oral intragastric intubation for two months, using RT-PCR assay. The current findings demonstrated that Trigonella reduced significantly glucose level in diabetic rats, while significantly increases insulin serum level. GCK expression levels in hepatic tissue of diabetic-rats are found to be suppressed, while Trigonella treatment induces an increase in hepatic GCK activity. Diabetes due to STZ has a little effect on IGF-1 gene expression, while Trigonella administration elevates mRNA expression significantly. On the other hand, Trigonella reduce and normalize the elevation of GLUT-2 gene expression, which increased due to STZ treatment. These results indicated that Trigonella are powerful antidiabetic agent, induce hypoglycemia by up-regulation of CGK and IGF-1 genes expression and induce an insulin mimetic activity