Water trees diagnostic of extruded underground cables: a case study in Saudi Arabia eastern province

In this paper, different electrical diagnostic techniques reported in the literature to predict the condition of extruded underground power cables are reviewed. Two of these techniques, namely the DC leakage current method and the DC conductivity method are applied to 14 underground cable samples rated between 15 kV and 69 kV collected from the Eastern Province of the Kingdom of Saudi Arabia. For this purpose, a setup has been constructed at the High Voltage Laboratory of King Fahd University of Petroleum & Minerals, Dhahran-Saudi Arabia. The results obtained show a correlation between the DC leakage current and the conductivity testing and the presence of water trees in the insulation material of cables. Samples suspected to have water trees are examined using the microscopic testing of the insulating material. The results obtained are in agreement with the electrical testing findings

Water trees diagnostic of extruded underground cables: a case study in Saudi Arabia eastern province

In this paper, different electrical diagnostic techniques reported in the literature to predict the condition of extruded underground power cables are reviewed. Two of these techniques, namely the DC leakage current method and the DC conductivity method are applied to 14 underground cable samples rated between 15 kV and 69 kV collected from the Eastern Province of the Kingdom of Saudi Arabia. For this purpose, a setup has been constructed at the High Voltage Laboratory of King Fahd University of Petroleum & Minerals, Dhahran-Saudi Arabia. The results obtained show a correlation between the DC leakage current and the conductivity testing and the presence of water trees in the insulation material of cables. Samples suspected to have water trees are examined using the microscopic testing of the insulating material. The results obtained are in agreement with the electrical testing findings

Analysis of water trees in underground HV cables using the KFUPMmicro-PIXE facility

Scanning with the micro-PIXE technique was employed to analyze water trees in the XLPE insulation of a field-aged underground HV cable. X-ray spectra of bow tie and vented water trees, the inner and outer semiconductive compounds, and an insulation spot free from any water tree were acquired. Simultaneously, two-dimensional elemental distribution profiles across the water trees were also measured. Various trace element impurities were identified in the analyzed spots and their possible sources are suggested. Differences in elemental distribution profiles in the scanned areas were observed and have been discussed on the basis of the mechanism of incorporation of these elements into the insulation. This study demonstrates the effectiveness of the micro-PIXE facility available in this laboratory in analyzing water trees in underground power cable

Analysis of water trees in underground HV cables using the KFUPMmicro-PIXE facility

Scanning with the micro-PIXE technique was employed to analyze water trees in the XLPE insulation of a field-aged underground HV cable. X-ray spectra of bow tie and vented water trees, the inner and outer semiconductive compounds, and an insulation spot free from any water tree were acquired. Simultaneously, two-dimensional elemental distribution profiles across the water trees were also measured. Various trace element impurities were identified in the analyzed spots and their possible sources are suggested. Differences in elemental distribution profiles in the scanned areas were observed and have been discussed on the basis of the mechanism of incorporation of these elements into the insulation. This study demonstrates the effectiveness of the micro-PIXE facility available in this laboratory in analyzing water trees in underground power cable

The images of psychiatry scale: development, factor structure, and reliability.

BACKGROUND: This analysis is based on a survey questionnaire designed to describe medical educators' views of psychiatry and psychiatrists. Our goals in this paper were to assess the psychometric properties of the survey questions by (a) using exploratory factor analysis to identify the basic factor structure underlying 37 survey items; (b) testing the resulting factor structure using confirmatory factor analysis; and (c) assessing the internal reliability of each identified factor. To our knowledge, this is the first attempt to use these techniques to psychometrically assess a scale measuring the strength of stigma that medical educators attached to psychiatry. METHODS: Survey data were collected from a random sample of 1,059 teaching faculty in 23 academic teaching sites in 15 countries. We conducted exploratory and confirmatory factor analysis to identify the scale structure and Cronbach's alpha to assess internal consistency of the resulting scales. RESULTS: Results showed that a two-factor solution was the best fit for the data. Following exploratory factor analysis, we conducted confirmatory factor analysis on a split half of the sample. Results highlighted several items with low loadings. Excluding factors with low correlations and allowing for several correlated variances resulted in a good fitting model explaining 95% of the variance in the data. CONCLUSIONS: We identified two unidimensional scales. The Images Scale contained 11 items measuring stereotypic content concerning psychiatry and psychiatrists. The Efficacy of Psychiatry Scale contained 5 items addressing perceptions of the challenges and effectiveness of psychiatry as a discipline

Atomic Resolution Cryo-EM Structure Of A Nativelike CENP-A Nucleosome Aided By An Antibody Fragment

Genomic DNA in eukaryotes is organized into chromatin through association with core histones to form nucleosomes, each distinguished by their DNA sequences and histone variants. Here, we used a single-chain antibody fragment (scFv) derived from the anti-nucleosome antibody mAb PL2-6 to stabilize human CENP-A nucleosome containing a native α-satellite DNA and solved its structure by the cryo-electron microscopy (cryo-EM) to 2.6 Å resolution. In comparison, the corresponding cryo-EM structure of the free CENP-A nucleosome could only reach 3.4 Å resolution. We find that scFv binds to a conserved acidic patch on the histone H2A-H2B dimer without perturbing the nucleosome structure. Our results provide an atomic resolution cryo-EM structure of a nucleosome and insight into the structure and function of the CENP-A nucleosome. The scFv approach is applicable to the structural determination of other native-like nucleosomes with distinct DNA sequences

A Novel Signaling Network Essential for Regulating Pseudomonas aeruginosa Biofilm Development

The important human pathogen Pseudomonas aeruginosa has been linked to numerous biofilm-related chronic infections. Here, we demonstrate that biofilm formation following the transition to the surface attached lifestyle is regulated by three previously undescribed two-component systems: BfiSR (PA4196-4197) harboring an RpoD-like domain, an OmpR-like BfmSR (PA4101-4102), and MifSR (PA5511-5512) belonging to the family of NtrC-like transcriptional regulators. These two-component systems become sequentially phosphorylated during biofilm formation. Inactivation of bfiS, bfmR, and mifR arrested biofilm formation at the transition to the irreversible attachment, maturation-1 and -2 stages, respectively, as indicated by analyses of biofilm architecture, and protein and phosphoprotein patterns. Moreover, discontinuation of bfiS, bfmR, and mifR expression in established biofilms resulted in the collapse of biofilms to an earlier developmental stage, indicating a requirement for these regulatory systems for the development and maintenance of normal biofilm architecture. Interestingly, inactivation did not affect planktonic growth, motility, polysaccharide production, or initial attachment. Further, we demonstrate the interdependency of this two-component systems network with GacS (PA0928), which was found to play a dual role in biofilm formation. This work describes a novel signal transduction network regulating committed biofilm developmental steps following attachment, in which phosphorelays and two sigma factor-dependent response regulators appear to be key components of the regulatory machinery that coordinates gene expression during P. aeruginosa biofilm development in response to environmental cues

Dysferlin Forms a Dimer Mediated by the C2 Domains and the Transmembrane Domain In Vitro and in Living Cells

Dysferlin was previously identified as a key player in muscle membrane repair and its deficiency leads to the development of muscular dystrophy and cardiomyopathy. However, little is known about the oligomerization of this protein in the plasma membrane. Here we report for the first time that dysferlin forms a dimer in vitro and in living adult skeletal muscle fibers isolated from mice. Endogenous dysferlin from rabbit skeletal muscle exists primarily as a ∼460 kDa species in detergent-solubilized muscle homogenate, as shown by sucrose gradient fractionation, gel filtration and cross-linking assays. Fluorescent protein (YFP) labeled human dysferlin forms a dimer in vitro, as demonstrated by fluorescence correlation spectroscopy (FCS) and photon counting histogram (PCH) analyses. Dysferlin also dimerizes in living cells, as probed by fluorescence resonance energy transfer (FRET). Domain mapping FRET experiments showed that dysferlin dimerization is mediated by its transmembrane domain and by multiple C2 domains. However, C2A did not significantly contribute to dimerization; notably, this is the only C2 domain in dysferlin known to engage in a Ca-dependent interaction with cell membranes. Taken together, the data suggest that Ca-insensitive C2 domains mediate high affinity self-association of dysferlin in a parallel homodimer, leaving the Ca-sensitive C2A domain free to interact with membranes