Studying Effects of Calcium Oxide Nanoparticles on Dentinogenesis in Male Wistar Rats

This study aimed to evaluate potential impacts of calcium oxide nanoparticles (CaO-NPs) at different dosages on predentin thickness, number of blood vessels, periodontal ligament thickness, and blood glucose level of Wistar rats. Twelve rats were randomly gathered into four groups, untreated (control) and CaO-NP-treated groups at three concentrations (25, 50, and 100 mg/kg of the body weight) over a period of 60 days. Histological investigation was performed on twenty-four lower incisor teeth extracted from all the tested groups under a light microscope, and an automatic Fujifilm was used to measure the blood glucose level. The results showed that regular nanoparticle treatment significantly increased predentin and periodontal ligament thicknesses, a gradual decrease in vascularization in the pulp tissue, and an increase in the blood glucose level as the dosages of nanoparticles administered to the rats increased. Administration of the CaO-NPs at low dosage (25 mg/kg) could be beneficial for the growth and integrity of teeth and dentinal tissues in rats

Studying Effects of Calcium Oxide Nanoparticles on Dentinogenesis in Male Wistar Rats

From Hindawi via Jisc Publications RouterHistory: publication-year 2021, received 2021-03-27, accepted 2021-07-18, pub-print 2021-07-26, archival-date 2021-07-26Publication status: PublishedThis study aimed to evaluate potential impacts of calcium oxide nanoparticles (CaO-NPs) at different dosages on predentin thickness, number of blood vessels, periodontal ligament thickness, and blood glucose level of Wistar rats. Twelve rats were randomly gathered into four groups, untreated (control) and CaO-NP-treated groups at three concentrations (25, 50, and 100 mg/kg of the body weight) over a period of 60 days. Histological investigation was performed on twenty-four lower incisor teeth extracted from all the tested groups under a light microscope, and an automatic Fujifilm was used to measure the blood glucose level. The results showed that regular nanoparticle treatment significantly increased predentin and periodontal ligament thicknesses, a gradual decrease in vascularization in the pulp tissue, and an increase in the blood glucose level as the dosages of nanoparticles administered to the rats increased. Administration of the CaO-NPs at low dosage (25 mg/kg) could be beneficial for the growth and integrity of teeth and dentinal tissues in rats

Impact of Peracetic Acid on The Dynamic Cyclic Fatigue of Heat-Treated Nickel-Titanium Rotary Endodontic Instrument

Peracetic acid (PAA) is widely used as a sterilising/disinfecting agent, and in endodontics, it has been introduced as a promising irrigant in root canal treatment. It has been used at different concentrations to achieve various functions. However, endodontic instruments in contact with PAA of a certain concentration may affect its fatigue resistance. Therefore, the aim of this study was to investigate the impact of PAA on the cyclic fatigue resistance of three commercial heat-treated nickel-titanium (NiTi) rotary files. Three types of heat-treated NiTi rotary files were selected: One Curve (OC), ProTaper gold (PTG), and Wave One Gold (WOG). Each type was divided into three subgroups (n= 6 for each file type): (1) untreated instruments; (2) immersed in 0.002% PAA; and (3) immersed in 0.35% PAA. The performance of each file type was tested in a simulated canal. The number of cycles to fracture (NCF) was determined to assess cyclic fatigue resistance of the files. Independent sample t-test was applied to compare each treated file within a subgroup with its respective control group, and one-way ANOVA was used to compare among the main groups. All types of tested files revealed a significant decline in the cyclic fatigue resistance after exposure to 0.002% PAA except the PTG (P = 0.209). After exposure of the files to a higher concentration (0.35% PAA), a dramatic reduction was demonstrated by all the groups. Before and after exposure of the files to PAA, PTG displayed the highest cyclic fatigue resistance followed by the WOG, while the OC showed the lowest resistance. Exposure of heated-treated NiTi files to PAA in a relatively high or low concentration adversely affects the cyclic fatigue resistance. The PTG files demonstrated the best performance among the tested types and can be disinfected with 0.002% PAA for clinical purpose

Assessing the Effects of Administering Calcium Oxide Nanoparticles on Oral Mucosa and Tongue of Male Wister Rats

The present study was conducted to evaluate the impact of calcium oxide-nanoparticles (CaO-NPs) administration at different concentrations on the epithelial thickness, papilla length and number of blood vessels of male Wister rats’ buccal mucosa, and the epithelial thickness, filiform length and number of blood vessels of the rats’ tongue. Twelve rats were used and randomly allocated into four groups: control group (untreated) and the three experimental groups were orally treated with CaO-NPs at different doses 25, 50 and 100 mg/kg of the body weight over two months. On the 61 st day, all the animals were sacrificed, and 1 cm of the buccal mucosa and the body of the tongue were carefully removed for histological analysis. Histological tissues were studied under a light microscope to investigate the impact of CaO-NPs administration on the oral tissues. An image processing software (Image J) was used to measure the epithelial thickness, papilla length and number of blood vessels of the rats’ buccal mucosa, and the epithelial thickness, filiform length and number of blood vessels of the rats’ tongue. The results showed that the CaO nanoparticles administration caused epithelial atrophy and decreased vascularization of buccal and tongue mucosa with all tested doses of CaO-NPs and the 100 mg/kg concentration showed the most significant effect