Evaluation of the Impact of Different Management Methods on Tetranychus urticae (Acari: Tetranychidae) and Their Predators in Citrus Orchards

To evaluate the effectiveness of eco-friendly treatments based on detergents classified as non-hazardous and black soap on the pest Tetranychus urticae Koch 1836, and their predators (Euseius stipulatus Athias-Henriot, 1960, Typhlodromus sp., Phytoseiulus persimilis Athias-Henriot, 1957), different treatments were applied to citrus orchards planted with Valencia late (Orange) in the Mechraa Belksiri region of Morocco (T0 = control experiment; T1 = spirodiclofen 0.5 L/Ha; T2 = 125 L/Ha (5%) of black soap; T3 = detergent; 4 L/Ha of Oni product + 2 L/Ha of Tide product). The results obtained during the whole monitoring period indicated that the three treatments used, namely spirodiclofen, black soap, and detergents, ensured a reduction in the rate of population of the pest T. urticae compared to the untreated plot. In the untreated plot, the average was 45.01 A± 4.90 mobile forms, while the plot treated with spirodiclofen it was only 21.10 C ± 2.71, the black soap 31.49 B ± 3.35, and in the plot treated with detergents, the average was similar to that obtained by spirodiclofen (22.90 C ± 2.18). On the predators (E. stipulatus, P. persimilis, and Typhlodropmus sp.), the black soap and the treatment with detergents were less harmful compared to the chemical spirodiclofen

Evaluation of the Impact of Different Management Methods on Tetranychus urticae (Acari: Tetranychidae) and Their Predators in Citrus Orchards

To evaluate the effectiveness of eco-friendly treatments based on detergents classified as non-hazardous and black soap on the pest Tetranychus urticae Koch 1836, and their predators (Euseius stipulatus Athias-Henriot, 1960, Typhlodromus sp., Phytoseiulus persimilis Athias-Henriot, 1957), different treatments were applied to citrus orchards planted with Valencia late (Orange) in the Mechraa Belksiri region of Morocco (T0 = control experiment; T1 = spirodiclofen 0.5 L/Ha; T2 = 125 L/Ha (5%) of black soap; T3 = detergent; 4 L/Ha of Oni product + 2 L/Ha of Tide product). The results obtained during the whole monitoring period indicated that the three treatments used, namely spirodiclofen, black soap, and detergents, ensured a reduction in the rate of population of the pest T. urticae compared to the untreated plot. In the untreated plot, the average was 45.01 A± 4.90 mobile forms, while the plot treated with spirodiclofen it was only 21.10 C ± 2.71, the black soap 31.49 B ± 3.35, and in the plot treated with detergents, the average was similar to that obtained by spirodiclofen (22.90 C ± 2.18). On the predators (E. stipulatus, P. persimilis, and Typhlodropmus sp.), the black soap and the treatment with detergents were less harmful compared to the chemical spirodiclofen

Ultrastructure of antennal sensillae of the samsum ant, Pachycondyla sennaarensis (Hymenoptera: Formicidae)

Black ant (Samsum), Pachycodyla sennarrensis, stings and injects venom and inflicts allergy (a rare clinical problem) due to its local and systemic reaction, which is considered as a health hazard amongst Saudi society. Thus, black ant is a source of serious concern for the government and experts as well.  Ultramorphological variations, distribution, differential sensillae counts (DSC) and total sensillae counts (TSC), were identified and estimated as a complementary part of the peripheral nervous system on the antennae of worker samsum ant, P. sennarrensis in order to understand its behavioral ecology. Based on scanning electron micrographs, four types of sensillae constituted with three trichoid types, which is an abundant form with a high distribution density at the apex, but a low density at subsequent proximal flagellomere of the antenna and a placoid type of sensillae (a rare form mostly found in the middle of the flagellum, that is, on the 4th, 5th and 6th flagellomere) were categorised. It is documented that nonporous trichoid type of sensillae are mechanoreceptors and thermoreceptors, whereas, the placoid types are olfactory receptors. Present findings in an indigenous species in Saudi Arabia may help in understanding the ecological behaviour of the ant, which subsequently may form the basis in producing its effective control measure in future.Key words: Samsum ants, Pachycondyla sennarrensis, ultrastructure, antenna, sensillae

Psiadia punctulata (DC.) Vatke induces cell apoptosis in highly metastatic MDA-MB-231 cells

Purpose: This study assessed the in vitro cell migration inhibitory and cell apoptotic effects of P. punctulata stem (PPS (and leaf hexane) PPL (extracts on breast cancer cell lines (MDA-MB-231 andMCF-7 cells).Methods: Cytotoxicity was quantified using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LD) release test after 48 h exposure of MDA-MB-231 cells to 0 – 200 μg/mL of PPS and PPL hexane extract. Cell apoptosis was determined using Muse™ cell cytometry, while the phytoconstituents of PPS and PPL hexane extracts were identified by gas chromatography–mass spectrometry.Results: The half-maximal inhibitory concentration (IC50) for PPS and PPL hexane extracts against MDA-MB-231 cells was 44.33 and 52.16 μg/mL, respectively. T, whereas the IC50 of PPS and PPL hexane extracts was 102.22 and 59.53 μg/mL against MCF-7 cells, sequentially. Treatment with 100 and 200 μg/mL of PPS and PPL hexane extract increased late apoptosis in MDA-MB-231 cells to 16.005 ± 1.155 and 52.58 ± 3.02 %, respectively, for PPS hexane extract and 77.34 ± 0 % and 95.21 ± 1.61 %, respectively, for PPL hexane extract, when compared to control cells (3.81% ± 0.79%). PPL hexane extract decreased cell migration and filled ~15.5 % of the wound gap on MDA-MB-231 cells after 24 h, while PPS hexane extract decreased cell migration by ~35 and ~42.5 % at 24 and 48 h, respectively. PPS and PPL hexane extracts contained several phytocompounds. Stem and leaf extracts of P. punctulata showed significant (p < 0.05) cell apoptotic and migration inhibition activities.Conclusion: The extracts P. punctulata exhibit potent cytotoxic activity against the tested breast cancer cells. Further research is required to assess the acute and subacute toxicity of the extracts

Larvicidal Activity of Selected Xerophytic Plants Against Culex pipiens and Aedes caspius (Diptera: Culicidae)

Abstract.-Methanol extracts of different plants namely, Trichodesma africanum (Boraginaceae), Cleome rupicola (Capparceae) and Ochradenus baccatus (Resedaceae), were tested for larvicidal activity against 4 th instar larvae of Aedes caspius and Culex pipiens mosquitoes. All plant extracts tested against Ae. caspius showed 100% mortality at 10µg/ml except the stem of O. baccatus which showed 90% mortality. However, most of the plant extracts tested against Cx. pipiens showed more than 50% mortality at 10µg/ml. Ae. caspius reported lower LD 50 than Cx. pipiens. The LD 50 of the extracts tested ranged between 5.3-0.99. The lowest LD 50 calculated against Ae. caspius was 1.2±0.06 and 0.99±0.16 µg/ml for the stem of T. africanum and C. rupicola, respectively. In conclusion, we have documented promising larvicidal potential of xerophytic plants, which could be considered as a potentially alternative source for developing novel larvicides to be used in controlling vectors of mosquito-borne diseases

Larvicidal Activity of Selected Xerophytic Plants Against Culex pipiens and Aedes caspius (Diptera: Culicidae)

Abstract.-Methanol extracts of different plants namely, Trichodesma africanum (Boraginaceae), Cleome rupicola (Capparceae) and Ochradenus baccatus (Resedaceae), were tested for larvicidal activity against 4 th instar larvae of Aedes caspius and Culex pipiens mosquitoes. All plant extracts tested against Ae. caspius showed 100% mortality at 10µg/ml except the stem of O. baccatus which showed 90% mortality. However, most of the plant extracts tested against Cx. pipiens showed more than 50% mortality at 10µg/ml. Ae. caspius reported lower LD 50 than Cx. pipiens. The LD 50 of the extracts tested ranged between 5.3-0.99. The lowest LD 50 calculated against Ae. caspius was 1.2±0.06 and 0.99±0.16 µg/ml for the stem of T. africanum and C. rupicola, respectively. In conclusion, we have documented promising larvicidal potential of xerophytic plants, which could be considered as a potentially alternative source for developing novel larvicides to be used in controlling vectors of mosquito-borne diseases

Larvicidal, ovicidal activities and histopathological alterations induced by Carum copticum (Apiaceae) extract against Culex pipiens (Diptera: Culicidae)

An experiment was carried out, firstly, to determine the possible toxicity of Carum copticum (Apiaceae) extract against Culex pipiens (Diptera: Culicidae), and, secondly, to study the histopathological alterations in the midgut of Cx. pipiens as a result of treatment with C. copticum extract. Larvicidal and ovicidal activities of C. copticum extract against the larvae of Cx. pipiens was determined according to World health organization (WHO). The inhibition effect of C. copticum was assessed by determining the mortality of the treated larvae and eggs. The histopathological effect of the C. copticum extracts on midgut epithelium of the larvae was examined under both light and transmission electron microscopy. The crude extract of C. copticum exerted 100% mortality for Cx. pipiens after 24 h at 200 μm/ml, and zero hatchability (100% mortality) at 150 μm/ml for Cx. pipiens. The histopathological study showed that larvae treated with C. copticum extract had cytopathological alterations of the midgut epithelium. The study provided information on various effects of C. copticum extract against Cx. pipiens

Phytochemical analysis and antibacterial activity of Washingtonia filifera (Lindl.) H. Wendl. fruit extract from Saudi Arabia

This work aimed to assess the antimicrobial potential of Washingtonia filifera extracts on some human pathogens. Agar well diffusion and minimum inhibitory concentrations (MIC) methods have been used to assess the antimicrobial activities of W. filifera extract against Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Escherichia coli, and Candida albicans. Only the ethyl acetate (ETAC) and methanol extracts revealed antimicrobial activity against tested microorganisms. S. aureus appears to be the most sensitive microbes to the ETAC extract with equal inhibition zone (30 mm) and MIC (65 µg/mL) values. This is followed by K. pneumoniae, E. coli, and A. baumanni, respectively. The plant extract had different phytochemical constituents such as alkaloids, sterols, and polyphenols. Column chromatography of the ETAC extract resulted in the loss of inhibitory effect at the highest concentration tested (50 mg/mL) against tested microorganisms. The haemolytic activity of the different extracts was found in the following order: Hexane (83.57%) > ETAC (35.71%) > chloroform (23.57143) > methanol (0.71%) based on the highest concentration tested (8.3 mg/mL). In conclusion, ETAC extract was the most promsing extract among extracts tested. Secondary plant metabolites are of great value as natural antimicrobial agents

Beetles associated with buried carcasses: potential forensic importance in Riyadh, Saudi Arabia

Burial has a special influence on carrion fauna, which may change the rate of decomposition and so affect postmortem estimates. This study aimed to identify species and succession of beetles on buried remains. From 28 January to 31 May 2021, we examined the Coleoptera on buried and exposed rabbit carcasses, on the campus of the University of King Saud in an area measured around 175 m by 250 m. The area is almost entirely devoid of flora, except for a few acacia trees and some common wild herbs. It is about 1 km away from residential neighborhoods. In total, 165 specimens belonging to 4 families were collected. The Tenebrionidae was the most abundant family, where it formed 52% at a depth of 20 cm and 78% at a depth of 40 cm in the buried carcasses, while the family Dermestidae was the most abundant in the surface carcasses, as it formed 53% of the insects sampled. Type Mesostena puncticollis Solier, 1835 (Tenebrionidae) predominated at exposed carcasses as well as at depths of 20, 40 cm and while type Dermestes frischii Kugelann, 1792 (Dermestidae), predominated at depth of 40 cm and exposed carcasses, and type Saprinus chalcites (Illiger, 1807) (Hisiteridae) at depth of 20 only. These findings are possibly useful in forensic investigations involving buried bodies in Riyadh, Saudi Arabia

Decomposition and dipteran succession on buried rabbits carcasses

The rabbit carcasses used in this study were buried at depths of 20 and 40 cm, were examined to construct a fly succession database on buried carrion in Riyadh, Saudi Arabia. Twenty-four rabbits were buried, 12 at 20 cm and 12 at 40 cm. One carcass at each depth was exhumed at 10-day intervals up to 120 days. The degradation rate varied among the carcasses. Differences in species and their colonization were also found in the superficial and exhumed carcasses. Eleven species of flies were recorded on carcasses interred at a depth of 20 cm and seven species at 40 cm, while 13 species were recorded on the carcasses over the top of the soil. Species Rhyncomya sp (Diptera: Calliphoridae), Sarcophaga dux Thomson, and Dolichotachina marginella (Wiedemann) (Diptera: Sarcophagidae) were dominant at both depths, while Chrysomya albiceps (Wiedeman), Chrysomya rufifaces (Macquart) (Diptera: Calliphoridae), Musca domestica Linnaeus, and Musca sorbens Wiedemann (Diptera: Muscidae) were dominant in surface carcasses. Megaselia scalaris (Loew) ((Diptera: Phoridae) is a common and typical forensic indicator that was found in the decay/advanced decay and dry stages at a depth of 20 cm. These findings are possibly useful in forensic investigations involving buried bodies in Riyadh, Saudi Arabia