Evaluation of salivary IL-10 and IL-8 as predictive biomarker in patient with oral and oropharyngeal squamous cell carcinoma

Background: The incidence and mortality rates of oral squamous cell carcinoma (OSCC) vary globally, yet the cancer continues to pose significant morbidity and mortality risks. This study sought to assess salivary IL-8 and IL-10 as biomarkers for identifying malignant oral lesions.  Methods: Between March 2022 and May 2023, a case-control study at the Dental Center of New Baquba, Diyala, Iraq, collected saliva for IL-8 and IL-10 measurement via ELISA. An independent t-test compared IL-8 and IL-10 means based on age and gender, while ROC analysis gauged specificity and sensitivity. Results: The prevalence of OSCC was evenly distributed across genders, with a higher occurrence among patients aged over 55 years (87.5%). OSCC distribution in the oral cavity indicated the lips as the most affected area (40.0%), followed by the tongue (37.0%), and the hard and soft palate (12.50%). Statistically significant differences were noted in IL-10 and IL-8 levels between the study and control groups across all age brackets, except for IL-8 in the age ≤55 (p ≤ 0.05). Elevated mean levels of IL-10 and IL-8 were observed in the older age group (>55), with IL-10 showing a greater increase in males (71.99±3.6), while IL-8 levels were higher in females. IL-10 demonstrated higher sensitivity (90%) and specificity (60%) with an optimal cutoff point value of 43.29, whereas IL-8 exhibited a sensitivity of 80% and a specificity of 47.5% with an optimal cutoff point value of 44.48. Conclusion: Individuals aged over 55 showed higher IL-10 and IL-8 levels, particularly in males for IL-10 and in females for IL-8. Salivary biomarkers hold promise for early OSCC detection

Evaluation of salivary IL-10 and IL-8 as predictive biomarker in patient with oral and oropharyngeal squamous cell carcinoma

Background: The incidence and mortality rates of oral squamous cell carcinoma (OSCC) vary globally, yet the cancer continues to pose significant morbidity and mortality risks. This study sought to assess salivary IL-8 and IL-10 as biomarkers for identifying malignant oral lesions.  Methods: Between March 2022 and May 2023, a case-control study at the Dental Center of New Baquba, Diyala, Iraq, collected saliva for IL-8 and IL-10 measurement via ELISA. An independent t-test compared IL-8 and IL-10 means based on age and gender, while ROC analysis gauged specificity and sensitivity. Results: The prevalence of OSCC was evenly distributed across genders, with a higher occurrence among patients aged over 55 years (87.5%). OSCC distribution in the oral cavity indicated the lips as the most affected area (40.0%), followed by the tongue (37.0%), and the hard and soft palate (12.50%). Statistically significant differences were noted in IL-10 and IL-8 levels between the study and control groups across all age brackets, except for IL-8 in the age ≤55 (p ≤ 0.05). Elevated mean levels of IL-10 and IL-8 were observed in the older age group (>55), with IL-10 showing a greater increase in males (71.99±3.6), while IL-8 levels were higher in females. IL-10 demonstrated higher sensitivity (90%) and specificity (60%) with an optimal cutoff point value of 43.29, whereas IL-8 exhibited a sensitivity of 80% and a specificity of 47.5% with an optimal cutoff point value of 44.48. Conclusion: Individuals aged over 55 showed higher IL-10 and IL-8 levels, particularly in males for IL-10 and in females for IL-8. Salivary biomarkers hold promise for early OSCC detection

Angiogenesis and MMP-2expression in Oral Squamous Cell Carcinoma&Verrucous Carcinoma and its Correlation with Clinicopathological Parameters

Background: An important step of tumor progression in which Matrix metalloproteinase have been implicated is angiogenesis, because these enzymes degrade the extracellular matrix and provide a permissive microenvironment for the growth of new blood vessels. The present study conducted to evaluate the immunohistochemical expression ofMatrixmetalloproteinase -2(MMP-2) andangiogenic marker (CD34) in Oralsquamous cell carcinoma(SCC)versus verrucous carcinoma(VC) and to correlate their expressions with the clinicopathological parameters. Material & Methods: MMP-2 and CD34 expression was examined immunohistochemically in twenty four paraffin tissue blocks of squamous cell carcinoma and verrucous carcinoma (twelve cases of each).  Results: All cases of Oral SCC exhibited positive   immunostaining for MMP-2, while only one case of VC showed –ve expression. Interestingly all cases of VC showed –ve MMP-2 immunostaining of the basal cell layer. Generally lymphatic vessels were more than blood vessels in both VC&SCC cases. The mean MMP-2 immunoexpression was (59.00%) for both stage I &stage II, while the higher CD34 immuno expression was in stage I. The mean expression of MMP-2 was higher in well differentiated OSCCs, while for CD34 it was higher in poorly differentiated OSCCs followed by moderately differentiated, then well differentiated, however no statistically significant difference was found. Non- significant correlation was found concerning the expression of both markers for both lesions. Conclusion: No statistical correlation was found between MMP-2 expression and angiogenesis in OSCC and OVC

2=Data.xlsx

Data used in the paper</p

Comparasion between Salivary biomarkers (macrophage inflammatory protein-1α and 1β ) in patients with oral lichen planus and patients with oral lichen planus and skin lesions

''Background'':((Oral.lichen.planus.(OLP).is.a.prevalent.chronic.inflammatory.oral.mucosal disease.that.is.T-cell.immune.driven..Oral.lichen.planus.has.not.yet.been.linked.to.a.specific etiology..It.has.been.suggestedthat.immune.cell-mediated.aberrances.are.the.cause..Macrophage inflammatory.protein-1α.(MIP -1α).and.macrophage.inflammatory.protein-.1β.(MIP- 1β).may.be involved.in.the.etiology.of.this.condition,.according.to.a.number.of.experts..As.a.predisposing factor.in.the.development.of.oral.lichen.planus, MIP -1α and.MIP- 1β.have.been.studied.for.their potential.effects))