Inhibitory Effect of Ethanolic Extract Isolated from Radix Pulasatillae Seeds against Proliferation of A549 Human Lung Cancer Cells

The present study was evaluated the anticancer activity of Radix Pulsatillae ethanol extract at first by using high throughput screening HTS for herb fractions screening in concentration of 100μM to identify the active fraction and then we measured the cytotoxic effects by using morphology changes, and detected apoptosis by Hoechst 33258 staining. Our results showed that Radix Pulsatillae ethanol extract has a strong toxicity against A549 lung cancer cells and low toxicity with mice normal splenocytes and requires more investigation to identify the small natural molecule with new anticancer activity and further mechanistic for cancer pathway

Inhibitory Effect of Ethanolic Extract Isolated from Radix Pulasatillae Seeds against Proliferation of A549 Human Lung Cancer Cells

The present study was evaluated the anticancer activity of Radix Pulsatillae ethanol extract at first by using high throughput screening HTS for herb fractions screening in concentration of 100μM to identify the active fraction and then we measured the cytotoxic effects by using morphology changes, and detected apoptosis by Hoechst 33258 staining. Our results showed that Radix Pulsatillae ethanol extract has a strong toxicity against A549 lung cancer cells and low toxicity with mice normal splenocytes and requires more investigation to identify the small natural molecule with new anticancer activity and further mechanistic for cancer pathway

The Effect of Nutrition on Immune System Review Paper

The most basic definition of immunity is the ability of the organism to resist the invasion of microorganisms and harmful substances. Blood plays an important role in immune development due to the presence of certain types of white blood cells in the blood and in some other tissues have the ability to eliminate strange organisms and destroy it as well the blood plasma contains antibodies made by certain immune cells to resist any strange proteins or any other substances linked to proteins, such proteins that stimulate the body tissues to form antibodies called antigen generators or antigens.Some objects work on precipitation these strange proteins to isolate them, so it called precipitins, while others work on the conglomerate of microorganisms or red blood cells. These are called agglutinins, while some act on neutralizing of toxins, called antitoxins.Immunizations are either natural immunity or innate immunity or acquired immunity. When the body resists the invasion of a particular species of microorganisms for the first time, it will have natural immunity, But the immunity acquired by the individual as a result of immunizations or vaccination of such microorganisms is known as acquired immunity, However, acquired immunity may be natural or artificial. The immunity generated in the individual an actual measles infection is known as naturally acquired immunity, but the immunity that is formed as a result of vaccinating the individual of typhoid or diphtheria vaccine is artificially acquired immunity (1, 2, 3, 4, 5).However, acquired immunity may be natural or artificial. The immunity generated in the individual following an actual measles infection is known as naturally acquired immunity (6). DOI: 10.7176/FSQM/90-06 Publication date:September 30th 201

Strengthening and Closing Cracks for Existing Reinforced Concrete Girders Using Externally Post-Tensioned Tendons

This research is devoted to study the strengthening technique for the existing reinforced concrete beams using external post-tensioning. An analytical methodology is proposed to predict the value of the effective prestress force for the external tendons required to close cracks in existing beams. The external prestressing force required to close cracks in existing members is only a part from the total strengthening force. A computer program created by Oukaili (1997) and developed by Alhawwassi (2008) to evaluate curvature and deflection for reinforced concrete beams or internally prestressed concrete beams is modified to evaluate the deflection and the stress of the external tendons for the externally strengthened beams using Matlab 7.0. The analytical investigation is implemented on three ideal reinforced concrete beam models, each model is considered to be strengthened using three types of external tendon profile (straight, draped and double draped), where each type of tendon profile is analyzed separately. No comparisons were made with analytical or experimental investigations, because no publications for this kind of studies were found

THE CYTOPROTECTIVE ACTIVITY OF AQUEOUS GREEN TEA EXTRACT AGAINST METRONIDAZOLE AND TINIDAZOLE GENOTOXIC EFFECT

Objective: The study was designed to evaluate the genoprotective effect of green tea extract against genotoxicity induced by metronidazole and tinidazole.Methods: A total of 36 mice were used, for each experiment. The animals were allocated into six groups: Group I - negative control administered distilled water; Group II - healthy mice treated with metronidazole alone, Group III - healthy mice treated with tinidazole alone, Group IV - healthy mice administered green tea extract alone, Group V - healthy mice administered of metronidazole anddd then green tea extract was administered, and Group VI - healthy mice administered of tinidazole and then green tea extract was administered.Results: It has been found that there are significant differences in mitotic index and micronucleus (MN) appearance between the Groups II, IV, and V (7.76±0.8, 11.92±1.14, and 8.36±0.57 and 6.92±0.5, 10.18±1.19, and 7.52±1.05) in bone marrow cells and spleen cells for mitotic index, respectively (6.75±0.4, 5.5±0.41, and 5.92±0.68), in bone marrow for MN appearance. Furthermore, it has been found that there are significant differences in mitotic index and MN appearance between the Groups III, IV, and VI (4.36±0.88, 11.92±1.14, and 5.36±0.55 and 4.08±0.35, 10.18±1.19, and 5.08±0.35) in bone marrow cells and spleen cells for mitotic index, respectively (8.43±0.96, 5.5±0.41, and 6.84±0.66), in bone marrow for MN appearance.Conclusion: The aqueous extract of green tea has a protective effect against the genotoxic effects induced by either metronidazole or tinidazole in mice

Molecular study of Acinetobacter baumannii Isolated from skin infection

     Eight  isolates of Acinetobacter baumannii were identified among one hundred fifty of Gram-negative bacteria grown on MacConkey agar that was isolated from different clinical skin specimens in Al-Najaf Al- Ashraf province hospitals. A.baumannii  identification  depends on morphological  ,microscopic examination  and biochemical  tests and confirmed by Api20E .The results showed that 60% of all  A.baumannii isolates produce biofilm and carry csuE gene  while 40% of all  A.baumannii isolates carry ompA gene

Jaceosidin Induces Apoptosis in U87 Glioblastoma Cells through G2/M Phase Arrest

Artemisia argyi is a widely used medicinal plant in China. The present study was designed to identify the bioactive constituents with antiglioma activity from leaves of Artemesia argyi. A bioactivity guided approach based on MTT assay for cells growth inhibition led to the isolation of a flavonoid, “jaceosidin” from ethanol extract of leaves of Artemesia argyi. The growth inhibitory effect of jaceosidin was explored using flow cytometry and Western blot studies. Our results showed that jaceosidin exerts growth inhibitory effect by arresting the cells at G2/M phase and induction of apoptosis. Furthermore, our study revealed that induction of apoptosis was associated with cell cycle arrest at G2/M phase, upregulation of p53 and Bax, decrease in mitochondrial membrane potential, release of cytochrome c, and activation of caspase 3. This mitochondrial-caspase-3-dependent apoptosis pathway was confirmed by pretreatment with caspase 3 inhibitor, Ac-DEVD-CHO. Our findings suggested that jaceosidin induces mitochondrial-caspase-3-dependent apoptosis in U87 cells by arresting the cell cycle at G2/M phase

Self-medication among medical students in Anbar and Fallujah Universities – Iraq

Background: Self-medication (SM) is a worldwide issue, that has serious adverse effects on individuals and communities. Objective:  To estimate the prevalence of self- medication   among medical student in Anbar and Falluja Universities and to explore the important reasons for using self-medication.  To identify the common sources and types of self-medication drugs. Method: A descriptive cross-sectional study was carried out among medical students in Anbar   and Fallujah Universities during the period from February to March 2018.  The subjects were asked to fill a questionnaire that consisted of questions on age, gender and educational level, in addition to questions for self-medication history. Results: The results revealed that 73% of medical students had practice of self- medication. A higher prevalence of self-medication was  found among 5th study year students. Antibiotics were the most frequent self-prescribe medicine that used by 137 (49.6%) of the participants, followed by analgesic (29%), and supplements by 40 (14.5%).  The most frequently given reasons for using self-medication were previous prescription and of pharmacists’ advice. Conclusion: A high prevalence of self- medication was found among medical students in Falluja & Anbar Universities with a statistical significant association between gender and self- medication practice

Evaluation of The Genoprotective Effect of Curcumin Against Methotrexate in Bone Marrow and Spleen Cells in Mice

Curcumin is a yellow pigment produced from the rhizomes of the Curcuma longa plant and a primary chemo preventive component of turmeric is used as a spice and food coloring ingredient. Curcumin has a large number of pharmacological activities, such as anticancer, anti-diabetic, antioxidant, anti-infectious, and anti-inflammatory properties.Investigation of the geno-protective effect of curcumin on methotrexate induces chromosomal aberrations of spleen and bone marrow cells. In this study, 32 mice were used and divided into four groups (eight mice at each group) as follows: Group1 (negative control): Dimethyl sulfoxide was given intraperitoneally to mice every day for ten days.Group2 (positive control): Mice were received a single dose (20mg/kg) of methotrexate intraperitoneally Group3: Mice were received (200mg/kg) of curcumin solution intraperitoneally for ten successive days.Group4: Mice were received (200mg/kg) of curcumin solution intraperitoneally for ten successive days and on the day 11, a single dose  of methotrexate ( 20 mg/kg ) was injected intraperitoneally . In animals treated with methotrexate, significant elevations of the chromosomal aberrations were observed along with a decline in the mitotic index. Meanwhile, there was a considerable elevation of the mitotic index and no detectable chromosomal changes in the curcumin-supplemented mice. The number of abnormal cells was reduced significantly in the curcumin-treated group in comparison with the methotrexate-treated group. The ability of curcumin to inhibit methotrexate's cytotoxic effects was shown by the compound's ability to raise the mitotic index. According to this finding, curcumin approved to be a protective agent against genotoxic effects of methotrexate. Keywords: Curcumin, Chromosomal aberrations, Methotrexate, Mitotic index, Mic