Mutation analysis of Phospholipase C Zeta (PLC?) in patients with low fertilisation rate

30th Annual Meeting of the European-Society-of-Human-Reproduction-and-Embryology (ESHRE) -- JUN 29-JUL 02, 2014 -- Munich, GERMANYWOS: 000359745300705…European Soc Human Reprod & Embryo

The effects of magnesium sulphate on the contractile activity of uterus in an animal model of preeclampsia

Aydos, Tolga Resat/0000-0002-1832-9336;WOS: 000343848700015PubMed: 24779245Purpose: This study was undertaken to evaluate the effects of magnesium sulfate (MgSO4) on the contractile activity of the uterus in a pregnant rat model of preeclampsia induced by N-nitro-arginine methyl ester (L-NAME). Materials and Methods: Twenty-eight, 160220 gram, three to four month old female Sprague-Dawley rats were used in this study. After conception was confirmed by vaginal smears on the first day of pregnancy, the animals were allocated into four groups according to the chemicals fed in their drinking water as control (nothing administered), L-NAME (50 mg/kg L-NAME), MgSO4 (600 mg/kg MgSO4), and MgSO4 + L-NAME group (600 mg/kg MgSO4 + 50 mg/kg L-NAME). The pregnant uterus strips were isolated on the 19th day and the contractile activity of uterus was examined by applying 0, 0.1, 0.2, 0.4, 0.8, and 2.5 mIU/mloxytocin to each group and responses are recorded accordingly. Results: There were no statistically significant differences regarding fetal parameters and peak amplitudes of the oxytocin stimulated pregnant rat myometrial strips among groups. In L-NAME group at 0 and 0.1 mIU/ml oxytocin, the contraction frequency in a ten-min period was statistically lower than the control group (Z = -2.850, p = 0.004; Z = -2.902, p = 0.004, respectively). In MgSO4 group only at 0 mIU/ml oxytocin, the frequencies of the contractions in ten-min period were statistically lower than the control group (Z = -2.973,p = 0.003). In L-NAME + MgSO4 group at 0, 0.1 and 0.2 mIU/ml oxytocin concentrations the frequencies of the contractions in ten-min period were statistically lower than the control group (Z = -4.018, p = 0.000; Z= -3.23'7,p = 0.001; Z= -2.902, p = 0.004, respectively). In L-NAME + MgSO4 given group at each oxytocin concentrations, the frequencies of the contractions in ten-min period were lower but not statistically different than the L-NAME group. Conclusion: MgSO4 has no significant effect on the amplitude of spontaneous or oxytocin induced myometrial contractions, but decreased the frequency of spontaneous contractions. At each doses of oxytocin, MgSO4 has no significant effect on the frequency of contraction in a pregnant rat model of preeclampsia induced by L-NAME.Kirikkale UniversityKirikkale University [2006/20]This study was supported by the Kirikkale University Research Fund Project No. 2006/20

The effects of magnesium sulphate on the contractile activity of uterus in an animal model of preeclampsia

Aydos, Tolga Resat/0000-0002-1832-9336;WOS: 000343848700015PubMed: 24779245Purpose: This study was undertaken to evaluate the effects of magnesium sulfate (MgSO4) on the contractile activity of the uterus in a pregnant rat model of preeclampsia induced by N-nitro-arginine methyl ester (L-NAME). Materials and Methods: Twenty-eight, 160220 gram, three to four month old female Sprague-Dawley rats were used in this study. After conception was confirmed by vaginal smears on the first day of pregnancy, the animals were allocated into four groups according to the chemicals fed in their drinking water as control (nothing administered), L-NAME (50 mg/kg L-NAME), MgSO4 (600 mg/kg MgSO4), and MgSO4 + L-NAME group (600 mg/kg MgSO4 + 50 mg/kg L-NAME). the pregnant uterus strips were isolated on the 19th day and the contractile activity of uterus was examined by applying 0, 0.1, 0.2, 0.4, 0.8, and 2.5 mIU/mloxytocin to each group and responses are recorded accordingly. Results: There were no statistically significant differences regarding fetal parameters and peak amplitudes of the oxytocin stimulated pregnant rat myometrial strips among groups. in L-NAME group at 0 and 0.1 mIU/ml oxytocin, the contraction frequency in a ten-min period was statistically lower than the control group (Z = -2.850, p = 0.004; Z = -2.902, p = 0.004, respectively). in MgSO4 group only at 0 mIU/ml oxytocin, the frequencies of the contractions in ten-min period were statistically lower than the control group (Z = -2.973,p = 0.003). in L-NAME + MgSO4 group at 0, 0.1 and 0.2 mIU/ml oxytocin concentrations the frequencies of the contractions in ten-min period were statistically lower than the control group (Z = -4.018, p = 0.000; Z= -3.23'7,p = 0.001; Z= -2.902, p = 0.004, respectively). in L-NAME + MgSO4 given group at each oxytocin concentrations, the frequencies of the contractions in ten-min period were lower but not statistically different than the L-NAME group. Conclusion: MgSO4 has no significant effect on the amplitude of spontaneous or oxytocin induced myometrial contractions, but decreased the frequency of spontaneous contractions. At each doses of oxytocin, MgSO4 has no significant effect on the frequency of contraction in a pregnant rat model of preeclampsia induced by L-NAME.Kirikkale UniversityKirikkale University [2006/20]This study was supported by the Kirikkale University Research Fund Project No. 2006/20

Surface runoff and carbonates-based definition of protection zones for Egirdir Lake in western Turkey

Freshwater of Isparta and Egirdir is supplied from the Egirdir Lake, which is the second largest freshwater lake of the Lakes District in Western Turkey. The Egirdir Lake has been studied within the framework of the Basin Protection Plan Special Provisions of the Egirdir Lake. The impact of runoff is taken into account in determining protection zones of the surface water reservoirs in Turkey. An approach that emphasizes the impact of groundwater flow in addition to the surface runoff has been adopted in this study. Water in Lake Egirdir is often classified as the Class II water according to terrestrial water resources quality criteria in Water Pollution Control Regulation of Turkey. The geological and hydrogeological studies reveal a significant amount of groundwater recharge into the Egirdir Lake through carbonate rocks and alluvial deposits outcropping in the basin, which is why Egirdir Lake still has a less contaminated water quality in spite of heavy pollutants. For this purpose, groundwater flow is prominently used in defining protection zones and surface runoff as well. The inner protection zone, which is defined as the 50-day travel time, and the outer protection zone, defined as the 400-day travel time, were estimated by infiltrometer and pumping tests in alluvium. Pumping tests results were used for the determination of hydraulic conductivities and groundwater levels for the determination of hydraulic gradients. Protection zones in karstic areas are based on the vulnerability map and large karstic springs