Validation and verification of LAMP, ISO, and VIDAS UP methods for detection of Escherichia coli O157:H7 in different food matrices

Escherichia coli O157:H7 is one of the most important food-borne pathogens to threaten public health. Cultural methods are used as a gold standard while they are laborious and time-consuming. Loop-mediated isothermal amplification (LAMP) method is an alternative method that became widely used for the detection of food-borne pathogens. The aim of this study was to evaluate the specificity and sensitivity of LAMP method for detection of E. coli O157:H7, also to compare detection performances with VIDAS UP and ISO (International Organization for Standardization) methods in different food matrices (beef meat, minced lamb meat, milk, cheese, apple puree, and soybean sprouts). E. coli O157:H7 were spiked in three different levels (high 4.58; medium 2.32; low 0.30 log10 CFU/g-ml) to food matrices. Although there were no significant differences in terms of the specificity and sensitivity values among the three methods (p ≥.05), it was determined that the highest specificity and sensitivity values obtained from the LAMP method. Sensitivity and specificity values of LAMP method were found as 0.997 and 0.988, for the ISO method were 0.989 and 0.971, and for the VIDAS UP method were 0.980 and 0.963, respectively. In milk samples, sensitivity and specificity values of the VIDAS UP method were significantly lower than LAMP and ISO methods (p <.05). However, there were no significant differences found for the other food matrices among the three methods (p >.05). It can be summarized from this study that specificity and sensitivity values of the LAMP method are equal or higher and less time-consuming than ISO and VIDAS UP methods. In conclusion, using a simple, fast, and inexpensive detection method, such as LAMP, especially in endemic regions or in an outbreak to control spreading of pathogens, is very important for public health. © 201

Acute Effects of Red Bull Energy Drinks on Atrial Electromechanical Function in Healthy Young Adults.

Energy drinks (EDs) are widely consumed by adolescents and young adults. Almost all kinds of arrhythmias have been reported following EDs consumption, most of which is atrial fibrillation (AF). Atrial conduction time prolongation and heterogeneous sinusal impulses propagation to the atriums are the key electrophysiological mechanisms leading AF. We aimed to evaluate the acute effects of Red Bull ED ingestion on atrial electromechanical conduction times in healthy young adults. After a 12-hour fasting, 54 healthy young adults consumed 330 mL of Red Bull ED. Atrial electromechanical coupling (PA), intra-atrial electromechanical delay (intra-AEMD), and interatrial electromechanical delay (inter-AEMD) were measured at baseline and 2-hour after Red Bull ED ingestion by echocardiographic tissue-Doppler imaging (TDI) method. PA-lateral (49.7 ± 11.2 vs 54.1 ± 11.0 msn, p = 0.001) and PA-septal (40.8 ± 9.1 vs 43.7 ± 10.5 msn, p = 0.032) times were statistically significantly prolonged after Red Bull ED ingestion. There was also a statistically significant increase in the duration of inter-AEMD (14.4 ± 10.6 vs 18.1 ± 8.5 msn, p = 0.010) after ED ingestion. It was showed that even a single can of ED can acutely increase atrial electromechanical conduction times in young adults. These findings may be the cause of ED-associated AF

Acute effects of red bull energy drinks on atrial electromechanical function in healthy young adults

Energy drinks (EDs) are widely consumed by adolescents and young adults. Almost all kinds of arrhythmias have been reported following EDs consumption, most of which is atrial fibrillation (AF). Atrial conduction time prolongation and heterogeneous sinusal impulses propagation to the atriums are the key electrophysiological mechanisms leading AF. We aimed to evaluate the acute effects of Red Bull ED ingestion on atrial electromechanical conduction times in healthy young adults. After a 12-hour fasting, 54 healthy young adults consumed 330 mL of Red Bull ED. Atrial electromechanical coupling (PA), intra-atrial electromechanical delay (intra-AEMD), and interatrial electromechanical delay (inter-AEMD) were measured at baseline and 2-hour after Red Bull ED ingestion by echocardiographic tissue-Doppler imaging (TDI) method. PA-lateral (49.7 ± 11.2 vs 54.1 ± 11.0 msn, p = 0.001) and PA-septal (40.8 ± 9.1 vs 43.7 ± 10.5 msn, p = 0.032) times were statistically significantly prolonged after Red Bull ED ingestion. There was also a statistically significant increase in the duration of inter-AEMD (14.4 ± 10.6 vs 18.1 ± 8.5 msn, p = 0.010) after ED ingestion. It was showed that even a single can of ED can acutely increase atrial electromechanical conduction times in young adults. These findings may be the cause of ED-associated AF. © 2019 Elsevier Inc