Microorganisms Resistant to White Phosphorus

We present preliminary results on the successful culturing of different microbial taxonomic groups on media containing white phosphorus (P4) as the sole source of phosphorus. The increase in culture resistance resulting from targeted selection was demonstrated. The highest concentration of P4 used in the study exceeds the threshold limit concentration of P4 in wastewater mud by 5000 times. Putative metabolites of P4 were also investigated. Keywords: biodegradation; white phosphorus; Aspergillus niger; Streptomyces sp. A

Biological Degradation of Yellow (White) Phosphorus, a Compound of First Class Hazard

Abstract: Biodegradation is an important method for the purification of industrial sewage and environment from chemical wastes. The biodegradation of elemental yellow (white) phosphorus was observed only in our studies. It is one of the most hazardous contaminants of environment. White phosphorus and its transformation products are used in industry, agriculture, drug manufacture, and military. For the first time, we have obtained cultures of microorganisms growing in media containing white phosphorus in concentration much higher than the threshold limit concentration in sewage. Elemental phosphorus is the strongest poison as reduced compounds and phosphate esters. However, in completely oxidized state (inorganic phosphates) it is a biogenic element necessary for all forms of life. Earth biomass consists of phosphorus almost by 3%. Prospects of the biodegradation of toxic phosphorus compounds, and elemental phosphorus are huge. The practical implementation of new deactivation method showing a number of advantages will allow one to reduce considerably fines imposed on plants producing and consuming yellow phosphorus

Fusarium oxysporum strains from wilting potato plants: Potential causal agents of dry rot disease in potato tubers

The present study focused on the isolation, identification and assessment of the dry rot-causing ability of Fusarium spp. associated with potato wilt in the Middle Volga region of Russia. A total of 14 Fusaria were isolated from the root necks of wilting potato plants. One isolate was identified as Fusarium solani, while the remaining thirteen of the isolates related to the species Fusarium oxysporum. Artificial inoculation of potato tubers with fungal spores showed that tubers of the cultivar Reggi exhibited the highest resistance [cultivar resistance index (CRI) = 0.78] to the tested isolates, while susceptibility (CRI = -0.44) was registered in tubers of the Zhukovskij rannij cultivar. The manifestation of dry rot during artificial inoculation with the spores of vascularassociated Fusaria was significantly influenced by pathogen virulence [H (9, N = 300) = 60.630, p <0.000001] and plant cultivar type [H (2, N = 210) = 17.201, p =0.00018]. However, not all isolates inhabiting occluded vessels caused dry rot in tubers

Data on the genome analysis of the probiotic strain Bacillus subtilis GM5

In the present study, we report data on the draft genome sequence of a lipopeptide producing rhizospheric Bacillus subtilis GM5 isolate. The genome consists of 4,271,280 bp with a GC-pair content of 43.3%. A total of 4518 genes including 75 tRNA genes, 3 operons coding for rRNA genes and 56 pseudogenes were annotated. Gene clusters responsible for the biosynthesis of secondary metabolites were validated. Six of the thirty-three clusters identified in the genome code for antimicrobial non-ribosomal peptides synthesis. The Whole Genome Shotgun project of B. subtilis GM5 has been deposited in the NCBI database under the accession number NZ_NKJH00000000 (https://www.ncbi.nlm.nih.gov/nuccore/NZ_NKJH00000000.1). Keywords: Bacillus subtilis, Analysis and assembly of the genome, Antimicrobial lipopeptide