Comparison of feeding diets diluted with sorghum-sudangrass silage or low-quality grass on nutrient intake and digestibility and growth performance of Holstein dairy heifers

This study was carried out to evaluate the nutrient intakes and growth of dairy heifers offered an alfalfa silage– corn silage diet (CON; 14.3% crude protein, 61.1% total digestible nutrients, 47.9% neutral detergent fiber) compared with diets containing 1 of 2 types of sorghumsudangrass (SS) silages: conventional or photoperiod sensitive. The objective of the study was to determine the potential to use SS to control dry matter (DM) and nutrient intakes and weight gain. Both diets were similar in nutrient composition, with approximately 13% crude protein, 60 to 61% total digestible nutrients, and 55% neutral detergent fiber. Seventy-two Holstein heifers (16–18 mo at study initiation) were blocked by initial body weight (light = 422 ± 12.8 kg; medium = 455 ± 14.8 kg; heavy = 489 ± 16.7 kg) with 3 pens assigned to each weight block (8 heifers/pen; 24 heifers/block). The 3 diets were randomly allocated to the pens within each block and offered for 12 wk. Heifers offered the CON diet had greater DM, protein, and energy intakes compared with those offered the SS silage-based diets due to the greater neutral detergent fiber concentration of the SS diets. With lower DM and nutrient intakes, average daily gain was in the recommended range (0.8– 1 kg/d for Holstein heifers) for heifers offered the SS silage-based diets (mean of 0.92 kg/d for both SS diets vs. 1.11 kg/d for CON). Sorting behaviors for heifers offered both SS diets were more aggressive against long, medium, and short particles compared with those of heifers offered the CON diet; however, heifers sorted large particles from photoperiod-sensitive silage more aggressively than those from conventional silage. Based on this study, SS silage-based diets can control the DM and energy intakes for heifers and maintain optimum growth rates, with harvesting at a shorter chop length likely helping to alleviate sorting issues

Comparison of feeding diets diluted with sorghum-sudangrass silage or low-quality grass on nutrient intake and digestibility and growth performance of Holstein dairy heifers

This study was carried out to evaluate the nutrient intakes and growth of dairy heifers offered an alfalfa silage– corn silage diet (CON; 14.3% crude protein, 61.1% total digestible nutrients, 47.9% neutral detergent fiber) compared with diets containing 1 of 2 types of sorghumsudangrass (SS) silages: conventional or photoperiod sensitive. The objective of the study was to determine the potential to use SS to control dry matter (DM) and nutrient intakes and weight gain. Both diets were similar in nutrient composition, with approximately 13% crude protein, 60 to 61% total digestible nutrients, and 55% neutral detergent fiber. Seventy-two Holstein heifers (16–18 mo at study initiation) were blocked by initial body weight (light = 422 ± 12.8 kg; medium = 455 ± 14.8 kg; heavy = 489 ± 16.7 kg) with 3 pens assigned to each weight block (8 heifers/pen; 24 heifers/block). The 3 diets were randomly allocated to the pens within each block and offered for 12 wk. Heifers offered the CON diet had greater DM, protein, and energy intakes compared with those offered the SS silage-based diets due to the greater neutral detergent fiber concentration of the SS diets. With lower DM and nutrient intakes, average daily gain was in the recommended range (0.8– 1 kg/d for Holstein heifers) for heifers offered the SS silage-based diets (mean of 0.92 kg/d for both SS diets vs. 1.11 kg/d for CON). Sorting behaviors for heifers offered both SS diets were more aggressive against long, medium, and short particles compared with those of heifers offered the CON diet; however, heifers sorted large particles from photoperiod-sensitive silage more aggressively than those from conventional silage. Based on this study, SS silage-based diets can control the DM and energy intakes for heifers and maintain optimum growth rates, with harvesting at a shorter chop length likely helping to alleviate sorting issues

Elevation of circulating serotonin improves calcium dynamics in the peripartum dairy cow.

Hypocalcemia is a metabolic disorder that affects dairy cows during the transition from pregnancy to lactation. Twelve multiparous Holstein cows and twelve multiparous Jersey cows were intravenously infused daily for approximately 7 days prepartum with either saline or 1.0mg/kg bodyweight of the immediate precursor to serotonin synthesis, 5hydroxy-l-tryptophan (5-HTP). On infusion days, blood was collected before, after, and at 2, 4, and 8h postinfusion. Blood and urine were collected daily before the infusion period, for 14 days postpartum and on day 30 postpartum. Milk was collected daily during the postpartum period. Feed intake and milk yield were unaffected by 5-HTP infusion postpartum. Cows infused with 5-HTP had elevated circulating serotonin concentrations prepartum. Infusion with 5-HTP induced a transient hypocalcemia in Jersey cows prepartum, but not in any other treatment. Holstein cows infused with saline had the highest milk calcium on the day of and day after parturition. Postpartum, circulating total calcium tended to be elevated, and urine deoxypyridinoline (DPD) concentrations were elevated in Holstein cows infused with 5-HTP. Overall, Jerseys had higher urine DPD concentrations postpartum when compared with Holsteins. Taken together, these data warrant further investigation of the potential therapeutic benefit of 5-HTP administration prepartum for prevention of hypocalcemia. Further research should focus on delineation of mechanisms associated with 5-HTP infusion that control calcium homeostasis during the peripartum period in Holstein and Jersey cows

Elevating serotonin pre-partum alters the Holstein dairy cow hepatic adaptation to lactation

<div><p>Serotonin is known to regulate energy and calcium homeostasis in several mammalian species. The objective of this study was to determine if pre-partum infusions of 5-hydroxytryptophan (5-HTP), the immediate precursor to serotonin synthesis, could modulate energy homeostasis at the level of the hepatocyte in post-partum Holstein and Jersey dairy cows. Twelve multiparous Holstein cows and twelve multiparous Jersey cows were intravenously infused daily for approximately 7 d pre-partum with either saline or 1 mg/kg bodyweight of 5-HTP. Blood was collected for 14 d post-partum and on d30 post-partum. Liver biopsies were taken on d1 and d7 post-partum. There were no changes in the circulating concentrations of glucose, insulin, glucagon, non-esterified fatty acids, or urea nitrogen in response to treatment, although there were decreased beta-hydroxybutyrate concentrations with 5-HTP treatment around d6 to d10 post-partum, particularly in Jersey cows. Cows infused with 5-HTP had increased hepatic serotonin content and increased mRNA expression of the serotonin 2B receptor on d1 and d7 post-partum. Minimal changes were seen in the hepatic mRNA expression of various gluconeogenic enzymes. There were no changes in the mRNA expression profile of cell-cycle progression marker cyclin-dependent kinase 4 or apoptotic marker caspase 3, although proliferating cell nuclear antigen expression tended to be increased in Holstein cows infused with 5-HTP on d1 post-partum. Immunofluorescence assays showed an increased number of CASP3- and Ki67-positive cells in Holstein cows infused with 5-HTP on d1 post-partum. Given the elevated hepatic serotonin content and increased mRNA abundance of <i>5HTR2B</i>, 5-HTP infusions may be stimulating an autocrine-paracrine adaptation to lactation in the Holstein cow liver.</p></div

Hepatic serotonin content and <i>TPH1</i> and <i>SERT</i> mRNA abundance of multiparous Holstein cows and multiparous Jersey cows administered pre-partum daily I.V. infusions of 1 liter of saline or 1 liter of 1.0 mg/kg bodyweight of 5-hyroxy-L-tryptophan (5-HTP) reconstituted in saline.

<p>Final treatment groups were saline-infused Holsteins (<i>n</i> = 6), 5-HTP infused Holsteins (<i>n</i> = 6), saline-infused Jerseys (<i>n</i> = 6), and 5-HTP infused Jerseys (<i>n</i> = 6). (A) Liver serotonin content corrected to ug of protein (B) mRNA abundance of tryptophan hydroxylase 1 (<i>TPH1</i>) and (C) mRNA abundance of serotonin reuptake transporter (<i>SERT</i>). Stars indicate statistical difference between group means (* = 0.05<<i>P</i><0.01). All values are reported as LS means ± SEM.</p

Primer sequences for genes quantified by real-time PCR.

<p>Primer sequences for genes quantified by real-time PCR.</p

Immunofluorescent images and counts of cells positive for Ki67 and CASP3 in multiparous Holstein cows and multiparous Jersey cows administered pre-partum daily I.V. infusions of 1 liter of saline or 1 liter of 1.0 mg/kg bodyweight of 5-hyroxy-L-tryptophan (5-HTP) reconstituted in saline.

<p>Final treatment groups were saline-infused Holsteins (<i>n</i> = 6), 5-HTP infused Holsteins (<i>n</i> = 6), saline-infused Jerseys (<i>n</i> = 6), and 5-HTP infused Jerseys (<i>n</i> = 6). (A) Percent of cells positive for Ki67 stain of all counted cells in representative images (<i>n</i> = 3) from each treatment group. (B) Immunofluorescent images of Ki67-positive cells. The scale bar indicates 15 microns. The arrow indicates an example of a cell considered positive for Ki67. d1 and d7 are days 1 and 7 post-partum, respectively. CON and 5-HTP represent saline-infused and 5-HTP infused groups, respectively. (C) Immunofluorescent images of CASP3-positive cells. The scale bar indicates 15 microns. The arrow indicates an example of a cell considered positive for CASP3. (D) Count of cells positive for CASP3 in representative images (<i>n</i> = 3) from each treatment group. Stars indicate statistical difference between group means (* = 0.05<<i>P</i><0.01; *** = 0.001<<i>P</i><0.0001). All values are reported as LS means ± SEM.</p

Hepatic mRNA abundance of <i>CCND1</i>, <i>CDK4</i>, <i>PCNA</i>, and <i>CASP3</i> in multiparous Holstein cows and multiparous Jersey cows administered pre-partum daily I.V. infusions of 1 liter of saline or 1 liter of 1.0 mg/kg bodyweight of 5-hyroxy-L-tryptophan (5-HTP) reconstituted in saline.

<p>Final treatment groups were saline-infused Holsteins (<i>n</i> = 6), 5-HTP infused Holsteins (<i>n</i> = 6), saline-infused Jerseys (<i>n</i> = 6), and 5-HTP infused Jerseys (<i>n</i> = 6). mRNA abundance of (A) cyclin D1 (<i>CCND1</i>) (B) cyclin-dependent kinase 4 (<i>CDK4</i>) (C) proliferating cell nuclear antigen (<i>PCNA</i>) and (D) caspase-3 (<i>CASP3</i>). All values are reported as LS means ± SEM.</p