Antifertility Effects of P-Alaxin in Male Adult Wistar Rats

P-alaxin, an artemisinin based combined therapy is very effective in treating malaria infection in areas of high resistance to conventional antimalarial drugs. It is a potentially promising anti-malaria drug that is composed of dihydroartemisinin and piperaquine phosphate. The present study investigates the fertility effects of therapeutic dose of P-alaxin. Fifteen male adult wistar rats of weighing between 150 and 210gm were grouped into three consisting of 5 males per group. The control group was orally administered with normal saline, the test and recovery groups were given body weight 15.4mg/kg of P-alaxin orally for three days after which the recovery group was allowed to recover from the drug’s effect for another three days. The animals were sacrificed twenty four (24) hours after the experiment.  The blood samples were collected through cardiac puncture.The result showed significant difference (p ≤0.05) in sperm count, sperm motility, sperm viability as well as in serum testosterone level of the male rats administered with P- alaxin and the recovery group when compared with the control group.The results suggest that oral administration of P – alaxin has significant negative effects (P <0.05) on sperm parameters and serum testosterone and could reduce male wistar rats fertility. Keywords: P-alaxin, artemisinin, antimalarial, sperm parameters, serum testosteron

Determining the stationary barriers to the implementation of radio frequency identification (RFID) technology in an emerging construction industry

Purpose To realize full benefits without sacrificing the practicality of such projects, the decision-making process for residential building construction needs to include sustainability principles at every level. Therefore, this paper aims to investigate the applicability of radio frequency identification (RFID) and identify the barriers that impede its successful adoption in construction projects to achieve sustainability. Design/methodology/approach This paper opted for a quantitative approach by using a structured questionnaire survey. A total of 107 responses were collected from Nigerian construction practitioners involved in private and public construction businesses. Findings The results showed the high cost of RFID implementation, with a mean score of 4.42 as the top-ranked barrier, followed by lack of security, maintenance, power availability and inadequate training. This study further deployed Ginni’s mean difference measure of dispersion and revealed that the stationary barrier to adopting RFID technology is the lack of demand. Practical implications The findings of this research can assist decision-makers in improving the sustainability of all building projects by implementing RFID. Originality/value The findings of this study will serve as the basis for comprehension and critically evaluate the numerous barriers preventing the widespread adoption of RFID technology

Determination of Work Index of Enyigba Lead Ore, Ebonyi State, South-East Nigeria

The determination of work index of Enyigba Lead Ore, Ebony State using the modified bond energy (Berry & Bruce) method was investigated. The test sample was obtained from Enyigba village in Ebonyi State. A reference material (granite) was sourced from Anguwan-Boro granite deposit, Sabo, Kaduna metropolis. A known weight of reference and test material were crushed, pulverized and ground using the laboratory ball mill machine. The cell used in grinding the two samples has twelve steel balls of diameter 3.5cm and 222g weight. The size analysis of the feed for both reference and test material was found to be 218.2 and 218.6µm, and a ball mill discharge was 189.9 and 188.9µm respectively. The granite as a reference material with work index of 15.13kwh/ short ton was used to calculate the work index of the test material which was found to be 14.37kwh/short ton, being the energy required to comminute one tone of the ore and confirmed to be within the range indicated by previous research work on lead ore

Comparative antioxidant activity, total phenol and total flavonoid contents of two Nigerian ocimum species

Antioxidants are compounds which act as a major defense against oxidative stress caused by free radicals. In this study, a comparative evaluation of the antioxidant properties, phenolic and flavonoid contents of the methanolic extracts of Ocimum gratissimum Linn and Ocimum canum Sims was carried out. Crude extracts of both plants were obtained by maceration of powdered plant materials in methanol (80%) for 24hrs. The phenolic and flavonoid contents were determined using standard methods while the antioxidant capacities were evaluated using six different in vitro radical scavenging assays: total antioxidant potential, reductive potential, I, 1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, lipid peroxidation inhibition, hydroxyl radical and nitric oxide scavenging activity. The total phenolic content in O. gratissimum and O. canum were found to be 32.66 ± 6.21 and 17.19 ± 2.54 mg GAE/g dw while total flavonoid content gave 1.94 ± 0.23 and 0.67 ± 0.01 mg QUE/g dw, respectively. Both extracts had effective reductive potential as well as exhibited strong total antioxidant capacity with increasing concentration of extract. Comparatively, O. gratissimum exhibited a significantly (p < 0.05) higher capacity to quench the DPPH free radical with IC50 value of 26.01 ± 3.2 µg/ml than O. canum, which has an IC50 value of 60.45 ± 5.22 µg/m. O. gratissimum also significantly inhibited membrane lipid peroxidation and hydroxyl radical formation with IC50 of 99.37 ± 8.56 µg/ml and 465.33 ± 21.62 µg/ml, respectively while O. canum correspondingly gave IC50 values of 447.5 ± 35.61 µg/ml and 868.16 ± 43.05 µg/ml. In the nitric oxide scavenging activity, however, O. canum showed a stronger inhibitory effect than O. gratissimum as indicated by their IC50 values of 277.22 ± 15.09 µg/ml and 731 ± 56.99 µg/ml, respectively. These activities are however several folds lower than those of butylated hydroxyl toluene (BHT), ascorbic acid and quercetin used as antioxidant standards. The results demonstrate that O. gratissimum has greater antioxidant capacity than O. canum because of its relatively higher radical scavenging activity in all antiradical tests carried out except the nitric acid scavenging test and higher contents of flavonoid and phenolic compounds. O. gratissimum is therefore more beneficial therapeutically than O. canum since it provides better defense against free radical induced oxidative stress, and this attribute probably explains its relative preference in ethnomedicine for both culinary and medicinal purposes amongst the Ocimum species widely cultivated in South Western Nigeria

Strategic issues in jatropha biofuel enterprise development in Nigeria

In Nigeria today, fossil fuels constitute the major source of energy for the economy with consumption costs in excess of N 654 billion for petrol, N 303.2 billion for diesel and N 194 billion for Kerosene. The global debate on Climate Change/CO 2 emissions and domestic concerns on economic, environmental and energy security implications have necessitated alterative energy options and created opportunities for sustainable biofuel enterprise in Nigeria. Jatropha curcas (known as Lapalapa in Yoruba-speaking parts of Nigeria) is an uncultivated non-food wild-species plant with great potential for bioenergy development in the country. The seeds are resistant to a high degree of aridity and contain 27-40% oil that can be processed to produce a high-quality biodiesel fuel, usable in a standard diesel engine. With estimated diesel demands in Nigeria being 3600-4200 thousand metric tonnes by 2020, there is an expansive opportunity for jatropha biofuel enterprise in the country. This study examines the strategic issues (technological, economic, environmental, and socio-cultural) governing its development and proposes policy recommendations for its successful exploitation

Early Detection of Paediatric Cancer: Equipping Primary Health‑Care Workers in Nigeria

Background: Paediatric cancer patients in Nigeria continue to arrive at specialist centers with advanced‑stage disease. The reasons for this are myriad, not least of which are delays in detection, diagnosis, and referral for treatment. While delayed presentation has often been reported from the perspective of delays from caregivers’ decisions, institutional deficiencies in the health care system may account for an unmeasured portion of the factors leading to delayed presentation. This project centered around training of health‑care professionals at community level to detect potential paediatric cancer signs and refer appropriately. Aim: The aim of the study is to access the immediate impact of training on early detection and referral of possible paediatric cancer cases in the community and primary level health care workers and professionals in the Southwest Nigeria. Materials and Methods: This was a retrospective review of the training impact in three South‑Western states in Nigeria. Scores before and after the training were analyzed using the IBM SPSS statistics, version 23 (IBM, Armonk, NY, USA). Results: A total of 732 primary health care workers were trained. In the pre assessment evaluations, 44.8% of participants reported that cancer did not occur in children, 47.2% did not know any referral pathway for a child suspected of cancer. The post training assessment indicated an improvement in participants’ understanding of common paediatric cancers types and how to refer a suspected case for specialist diagnosis and attention. Mean scores before and after the training were 3.5/15 and 12.5/15, respectively. Conclusion: Training health-care professionals working at the community level can have an immediate and measurable impact on early detection and referral for paediatric cancers, as seen by the difference in pre training and post training assessment scores. There remains a need for continuous training to ensure early referral and ultimately increase survival indices of children diagnosed with cancer in Nigeria

Gastroprotective Effects of the Aqueous Seed Extract of Entada gigas (Linn.) Fawc. and Rendle (Fabaceae) in Ulcer Models in Rats

Background: The extract of Entada gigas Linn. (Fabaceae) is used for the treatment of ulcer in Nigerian local medicine. Objectives: This study investigated the gastroprotective effects of the aqueous seed extract of E. gigas on gastric ulcers in rats. Methodology: The ethanol- (EIU), pylorus ligation- (PLIU) and cold restraint stress (CRSIU)-induced ulcer models were used. The aqueous seed extract was administered at doses of 50-400 mg/kg p.o. Estimations of gastric content volume, pH and titratable acidity in PLIU test and ulcer score/index in all models were done. Results: In the EIU test, E. gigas produced significant reduction (p &lt; 0.05) in ulcer scores, with peak effect elicited at 100 mg/kg (2.30 ± 0.99; 87.22% inhibition), compared with control (10.80 ± 0.80). This effect was comparable to that of misoprostol (3.00 ± 1.31; 83.33% inhibition). In the PLIU test, the extract caused significant reduction (p &lt; 0.05) in the ulcer score compared with control (6.30 ± 0.70). Peak effect was elicited at the dose of 200 mg/kg (ulcer score 2.90 ± 0.83; 63.17%). This effect was comparable to that of cimetidine (2.60 ± 0.93; 66.98%). Also, the extract at 50 and 200 mg/kg, and cimetidine 100 mg/kg (0.24 ± 0.07, 1.06 ± 0.22 and 1.02 ± 0.16 mL/4 h, respectively) significantly reduced (p &lt; 0.05) the volume of gastric content relative to control (2.32 ± 0.33 mL/4 h). In the CRSIU test, E. gigas caused significant reduction (p &lt; 0.05) in the ulcer score, with the greatest effect produced at the dose of 50 mg/kg (0.80 ± 0.49; 92.38% inhibition), relative to control (4.20 ± 0.64). This effect was comparable to that of misoprostol (1.40 ± 0.60; 80% inhibition). Conclusion: The aqueous seed extract of E. gigas possess significant antiulcer activity mediated via cytoprotective and anti-secretory mechanisms. Keywords: Entada gigas, Fabaceae, ulcer, gastroprotective effect, cytoprotective, anti-secretory