Validation of plasma protein glycation and oxidation biomarkers for the diagnosis of autism

Autism Spectrum Disorder (ASD) is a common neurodevelopmental disorder in children. It is currently diagnosed by behaviour-based assessments made by observation and interview. In 2018 we reported a discovery study of a blood biomarker diagnostic test for ASD based on a combination of four plasma protein glycation and oxidation adducts. The test had 88% accuracy in children 5–12 years old. Herein, we present an international multicenter clinical validation study (N = 478) with application of similar biomarkers to a wider age range of 1.5–12 years old children. Three hundred and eleven children with ASD (247 male, 64 female; age 5.2 ± 3.0 years) and 167 children with typical development (94 male, 73 female; 4.9 ± 2.4 years) were recruited for this study at Sidra Medicine and Hamad Medical Corporation hospitals, Qatar, and Hospital Regional Universitario de Málaga, Spain. For subjects 5–12 years old, the diagnostic algorithm with features, advanced glycation endproducts (AGEs)—Nε-carboxymethyl-lysine (CML), Nω-carboxymethylarginine (CMA) and 3-deoxyglucosone-derived hydroimidazolone (3DG-H), and oxidative damage marker, o,o’-dityrosine (DT), age and gender had accuracy 83% (CI 79 – 89%), sensitivity 94% (CI 90–98%), specificity 67% (CI 57–76%) and area-under-the-curve of receiver operating characteristic plot (AUROC) 0.87 (CI 0.84–0.90). Inclusion of additional plasma protein glycation and oxidation adducts increased the specificity to 74%. An algorithm with 12 plasma protein glycation and oxidation adduct features was optimum for children of 1.5–12 years old: accuracy 74% (CI 70–79%), sensitivity 75% (CI 63–87%), specificity 74% (CI 58–90%) and AUROC 0.79 (CI 0.74–0.84). We conclude that ASD diagnosis may be supported using an algorithm with features of plasma protein CML, CMA, 3DG-H and DT in 5–12 years-old children, and an algorithm with additional features applicable for ASD screening in younger children. ASD severity, as assessed by ADOS-2 score, correlated positively with plasma protein glycation adducts derived from methylglyoxal, hydroimidazolone MG-H1 and Nε(1-carboxyethyl)lysine (CEL). The successful validation herein may indicate that the algorithm modifiable features are mechanistic risk markers linking ASD to increased lipid peroxidation, neuronal plasticity and proteotoxic stress.</p

American Thoracic Society 2019 Pediatric Core Curriculum

The American Thoracic Society Pediatric Core Curriculum updates clinicians annually in pediatric pulmonary disease in a 3 to 4 year recurring cycle of topics. The 2019 course was presented in May during the Annual International Conference. An American Board of Pediatrics Maintenance of Certification module and a continuing medical education exercise covering the contents of the Core Curriculum can be accessed online at www.thoracic.org.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/152541/1/ppul24482_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/152541/2/ppul24482.pd

UV‐generated hot electrons in Au‐ZnO as robust way for plasmon‐enhanced photoluminescence and photocatalysis reactions in metal‐semiconductor nanomaterials

International audienceHere, we introduce a mechanistic study to design the hybrid junction in metallic‐semiconductor (M/SC) nanostructures. UV light induced hot electrons generation in ZnO nanostructures is precisely tuned the photoluminescence (PL) and photocatalytic (PC) properties in hybrid Au/ZnO nanomaterials. Both, enhancement and quenching of the PL and PC functionalities are obtained, depending on the Au nanoparticles (AuNPs) characteristics and Au/ZnO molecular distance. Basically, under UV irradiation free‐ligand AuNPs quench the luminescence of ZnONPs through direct charge transfer (CT) from ZnO into AuNPs. Whereas, capped AuNPs enhance the ZnO emission through the indirect CT from AuNPs into ZnO ones facilitated by the distance created by the CTAB ligand between both constituents of the hybrid systems. An optimal Au/ZnO molecular distance is suitable to enhance both the plasmonic photocatalysis reaction and photoelectric properties of M/SC nanostructures. This phenomena is mediated by the energy transfer (ET) from ZnONPs into AuNPs. The resulting PL enhancement is described by the plasmon induced resonance energy transfer effect (PIRET effect)

Enhanced Photocatalytic Activity and Photoluminescence of ZnO Nano-Wires Coupled with Aluminum Nanostructures

In this study, we fabricated a hybrid plasmonic/semiconductor material by combining the chemical bath deposition of zinc oxide nanowires (ZnONWs) with the physical vapor deposition of aluminum nanostructures (AlNSs) under controlled temperature and atmosphere. The morphological and the optical properties of the ZnONWs/AlNSs hybrid material fabricated at different temperatures (250, 350, and 450 &deg;C) and thicknesses (5, 7, and 9 nm) of Al layers were investigated. By adjusting the deposition and annealing parameters, it was possible to tune the size distribution of the AlNSs. The resonant coupling between the plasmonic AlNSs and ZnONWs leads to an enhanced photoluminescence response. The photocatalytic activity was studied through photodegradation under UV-light irradiation of methylene blue (MB) adsorbed at the surface of ZnO. The MB photodegradation experiment reveals that the ZnONWs covered with 7 nm aluminum film and annealed at 450 &deg;C exhibit the highest degradation efficiency. The comparison between ZnONws and ZnONws/AlNSs shows a photoluminescence enhancement factor of 1.7 and an increase in the kinetics constant of photodegradation with a factor of 4

Size controlled photoemission and photocatalytic activity of Au-ZnO nanostructures

International audienc

Epidemiology of neonatal near miss in Ethiopia: A systematic review and meta-analysis

Background: In many low-resource countries, progress toward reducing neonatal mortality is slow. This scenario is particularly prevalent in Sub-Saharan Africa and Ethiopia. Neonates who nearly died due to severe complications during birth or the neonatal period constitute neonatal near-miss cases. However, no pooled data exists to quantify the magnitude of neonatal near-misses in Ethiopia. As a result, the goal of this study was to identify national burden of NNM and associated factors in Ethiopia. Methods: This systematic review and meta-analysis follows the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline and was registered on PROSPERO (CRD42022303477). A comprehensive search of literature was carried out using PubMed/Medline, HINARI, African Journal Online, Science Direct, and Google Scholar. From 2010 to 2020, all lists of eligible studies' references were also identified and retrieved. The pooled estimate with a (95% CI) was calculated using a random-effects model in STATA 14.1-version. I2and meta-bias statistics were used to assess heterogeneity of the included studies. Results: From those included studies, (9 articles) with a total of 6437 neonates were included in the meta-analysis. The pooled prevalence of NNM in Ethiopia was 14% (95% CI: 13–15). Neonates who were born by Caesarean section, neonates whose mothers had pregnancy-induced hypertension and premature ruptured-membranes had higher odds of NNM. Conclusions: In Ethiopia, one out of every seven neonates suffers from NNM. Cesarean section, Pregnancy-induced hypertension and premature ruptures of the membrane are risk factors for NNM. Improving maternal health-care utilization would reduce the burden of NNM

Pure, Size Tunable ZnO Nanocrystals Assembled into Large Area PMMA Layer as Efficient Catalyst

Here, we demonstrate for the first time a strategy to self-assemble ZnO nanoparticles (NP) on a large area by a facile one-step process. First, rough and random ZnO nanocrystals (NC), were produced by free-stabilizing aqueous synthesis. Therefore, a post thermal treatment at various temperatures ranging from 80 to 800 &#176;C was necessary to obtain size-tunable and photoluminescent crystalline NP. The fabricated NP had both efficient UV photoluminescence and photocatalytic activity by photo-degradation of Methylene Blue (MB) dye. The annealed NP showed an absorption blue shift in the UV region with decreasing size. This shift was attributed to high quantum confinement effect since ZnO NP diameter reached values lower than the Bohr radius of ZnO (~2.7 nm). The photocatalytic activity displayed dependency on the particle&#8217;s size, number, and crystallinity. Subsequently, the NP were self-assembled inside poly(methyl methacrylate) (PMMA) nanoholes. Subsequently, large area substrate of homogenous properties ZnO NP was obtained. Moreover, the synthesis facility, photoemission and photocatalytic properties of ZnO NP could be a new insight into the realization of high performance and low cost UV laser devices

Knowledge, Attitudes, and Practices Regarding COVID-19 Among Health Care Workers in Public Health Facilities in Eastern Ethiopia: Cross-sectional Survey Study

BackgroundOn March 13, 2020, Ethiopia reported the first confirmed case of COVID-19 in Addis Ababa. COVID-19 is likely to overwhelm an already-fragile health care delivery system and reduce the availability of essential health services. This analysis of data from the Somali Region of Eastern Ethiopia on health care workers’ (HCWs) knowledge, attitudes, and practices regarding the prevention and control of COVID-19 may be used in planning health education programs about the emerging viral disease. ObjectiveThis study aimed to investigate the knowledge, attitudes, and practices of HCWs regarding COVID-19 infection. MethodsThis cross-sectional study was conducted among HCWs in three public health facilities in the Somali Region, Eastern Ethiopia. A self-administered questionnaire was shared with all HCWs working at the public health facilities. A total of 15 knowledge questions were scored as 1 or 0 for correct or incorrect responses, respectively. A total of 14 practice questions were scored on a 3-point scale from 1 (“always”) to 3 (“never”). A total of six attitude questions were rated on a 5-point Likert scale, in a negative dimension, as follows: 1 (“strongly agree”), 2 (“agree”), 3 (“neutral”), 4 (“disagree”), and 5 (“strongly disagree”). Mean scores were calculated and used as a cut point to dichotomize the outcome variables (>13.7 indicated good knowledge, <18.8 indicated good practices, and ≤10.5 indicated favorable attitudes). We used t tests and analyses of variance (ie, F tests) to analyze the mean score differences of knowledge, attitudes, and practices between the independent variables. Spearman correlation was used to assess the relationship between mean knowledge and attitude scores. ResultsOf the 686 HCWs approached, a total of 434 HCWs responded (63.3% response rate). The mean age of the participants was 27.6 (SD 5.3) years, and the majority of the participants were male (293/434, 67.5%). The mean knowledge score was 13.7 (SD 2.6), and 73.3% (318/434) of participants had sufficient knowledge. The mean attitude score was 10.5 (SD 4.1), and 54.8% (238/434) of the participants had a good attitude toward COVID-19. The mean practice score was 18.8 (SD 5.8), and 61.5% (267/434) of the participants practiced precautionary measures to prevent COVID-19. There was a negative correlation between knowledge and attitude scores (r=–0.295, P<.001) and between knowledge and practice scores (r=–0.298, P<.001). ConclusionsThe overall levels of knowledge and practice were relatively better than the attitude level. This highlights the need to implement strategies that enhance the positive attitudes and safe practices of the HCWs for better containment of the pandemic and supporting of essential health care services

Global variation in postoperative mortality and complications after cancer surgery: a multicentre, prospective cohort study in 82 countries

© 2021 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY-NC-ND 4.0 licenseBackground: 80% of individuals with cancer will require a surgical procedure, yet little comparative data exist on early outcomes in low-income and middle-income countries (LMICs). We compared postoperative outcomes in breast, colorectal, and gastric cancer surgery in hospitals worldwide, focusing on the effect of disease stage and complications on postoperative mortality. Methods: This was a multicentre, international prospective cohort study of consecutive adult patients undergoing surgery for primary breast, colorectal, or gastric cancer requiring a skin incision done under general or neuraxial anaesthesia. The primary outcome was death or major complication within 30 days of surgery. Multilevel logistic regression determined relationships within three-level nested models of patients within hospitals and countries. Hospital-level infrastructure effects were explored with three-way mediation analyses. This study was registered with ClinicalTrials.gov, NCT03471494. Findings: Between April 1, 2018, and Jan 31, 2019, we enrolled 15 958 patients from 428 hospitals in 82 countries (high income 9106 patients, 31 countries; upper-middle income 2721 patients, 23 countries; or lower-middle income 4131 patients, 28 countries). Patients in LMICs presented with more advanced disease compared with patients in high-income countries. 30-day mortality was higher for gastric cancer in low-income or lower-middle-income countries (adjusted odds ratio 3·72, 95% CI 1·70–8·16) and for colorectal cancer in low-income or lower-middle-income countries (4·59, 2·39–8·80) and upper-middle-income countries (2·06, 1·11–3·83). No difference in 30-day mortality was seen in breast cancer. The proportion of patients who died after a major complication was greatest in low-income or lower-middle-income countries (6·15, 3·26–11·59) and upper-middle-income countries (3·89, 2·08–7·29). Postoperative death after complications was partly explained by patient factors (60%) and partly by hospital or country (40%). The absence of consistently available postoperative care facilities was associated with seven to 10 more deaths per 100 major complications in LMICs. Cancer stage alone explained little of the early variation in mortality or postoperative complications. Interpretation: Higher levels of mortality after cancer surgery in LMICs was not fully explained by later presentation of disease. The capacity to rescue patients from surgical complications is a tangible opportunity for meaningful intervention. Early death after cancer surgery might be reduced by policies focusing on strengthening perioperative care systems to detect and intervene in common complications. Funding: National Institute for Health Research Global Health Research Unit