181 research outputs found
On-site analysis for nitrogen oxides using a newly developed portable flow injection analyzer
A new portable flow injection system was developed for on-site chemical analysis, which allows the rapid analysis of aqueous samples at sampling sites. The system comprises a
newly designed double-plunger micro pump, a six-way sample injector, a reaction coil in a thermostated compartment and a detector. All of these units are connected with 0.5
mmi.d. PTFE tubing. A visible detector is assembled using a maximum wavelength of 525 nm of a light-emitting diode (LED) and 540 nm of an interference filter. The system
is of the one-box type, whose dimensions are 160(W) x 160(H) x 320(D) mm, and is easy to carry to the analysis site; the weight is 8 kg. A thermostated compartment is
incorporated into the proposed system in order to be used outdoors, where temperatures are changeable. The system can workwith DC 12Vas well as AC 100V; therefore, a car
battery or a portable battery can be used as the power source. The analytical data can be memorized in IC cards or a note-book personal computer connected with a RS232C
cable. Furthermore, software on the market can be easily used. By using the proposed system, the on-site determination of nitrogen oxides, such as nitrate and nitrite, could be carried out. Calibration graphs for nitrate and nitrite ions were linear over ranges of 0
to 1.0ppm and 0 to 100ppb ofN-NOs and N-NO2 with good precision; the sampling rate was 40~50 samples per hour. The detection limit for N-NO3 and N-NO2 was 0.5 ppb. By using the proposed system, the on-site determination of nitrate and nitrite in river water samples was carried out. The relative standard deviations of ten injections
were 0.65% for nitrate and 0.15% for nitrite. Furthermore, nitrate and nitrite in biological samples as a metabolic products of nitric oxide, which have attracted a lot of attension as a messenger of diverse physiological processes, were also determined on-site with
high sensitivity. By using a car battery as a power source, the proposed system worked continuously. In addition, it worked for about 5 hours continuously with a portable battery.試料採取現場での迅速な分析,すなわちオンサイト分析のための新しいポータブルフローインジェクション分析計の開発を行った・このFIA装置では,160×160誉320(mm)の箱の中にダブルプランジ
ャーマイクロポンプ,試料注入語検出器及び反応恒温槽を組み入れ,重量8kgと小型・軽量化を達成し,持ち運び容易な分析計とした.特に,525nmの発光ダイオード(LED)を光源とする新規検出
器を開発することにより,小型,省電力化が可能となった.温度変化の大きな屋外での測定に耐えられるように,反応恒温槽も装備した.本FIA装置は交流100Vでのほか,直流12Vでも稼働し,カーバ
ッテリーや市販のポータブルバッテリーを電源とするオンサイト分析に対応できるよう設計した.カーバッテリーを電源とした場合は長時間の連続運転が可能である.測定データはメモリーカードや
RS232Cによりノート型パーソナルコンピュータへの通信も可能で,更に市販の表計算ソフトを利用してデータ処理が可能である.本装置を窒素酸化物のオンサイト分析に応用した.硝酸,亜硝酸イオン標準液による検量線は,0~1・Oppm,0~100ppbの範囲で直線性,再現性共に良好で,1時間当たり40~50試料の分析が可能であった・検出限界(5/Ⅳ=3)は硝酸,亜硝酸態窒素として0.5ppbと通常のFIAシステムを用いる場合と全く変わらない優れた性能を有することが分かった.河川水中の硝酸,亜硝酸イオンのオンサイト分析では10回の繰り返し測定における相対標準偏差は0.65%及び0.15%と良好な結果が得られた.また,血清中の一酸化窒素(NO)の酸化代謝物としての硝酸,亜硝酸イオン濃度のオンサイト分析を行い,良好な結果を得た.本研究で検討したポータブルバッテリーでは1回の充電で5時間の連続運転が可能であった
Bone erosions in rheumatoid arthritis can be repaired through reduction in disease activity with conventional disease-modifying antirheumatic drugs
We conducted the present study to determine whether repair of erosions occurs in patients with rheumatoid arthritis (RA) treated with conventional disease-modifying anti-rheumatic drugs (DMARDs) and to compare clinical characteristics between patients exhibiting and not exhibiting erosion repair. We included in the study a total of 122 RA patients who fulfilled the 1987 American College of Rheumatology criteria for RA; all patients had paired sequential radiographs of both hands and wrists showing erosive changes at baseline. Patients were classified into two groups according to the presence of repair of erosions at follow up, namely the 'repair observed' and 'repair not observed' groups. Clinical characteristics, disease activity, radiographic scores and treatment in the two groups were compared. Forty-four repairs were observed in 13 patients (10.7%). Compared with the repair not observed group, the functional class of the patients in the repair observed group was lower at baseline (P < 0.01) and the mean disease activity was lower at follow up (P < 0.005). The changes in radiographic scores per year (total radiographic score and erosion score) were lower (P < 0.05 and P < 0.01, respectively) in the repair observed group. No difference in treatment was observed. Repair of erosions was detected in 10.7% of RA patients treated with conventional DMARDs. Repairs were associated with low functional class at baseline and low disease activity at follow up. These observations support the importance of reduction in disease activity in RA patients. Because repair of erosions was detected in a substantial number of patients, assessment of erosion repair should be incorporated into the radiographic evaluation and scoring of RA
Linear Basal Cell Carcinoma: A Case Report
Basal cell carcinoma (BCC) presents with diverse clinical features, and several morphologic and histologic variants of BCC have been reported [Sexton et al.: J Am Acad Dermatol 1990;23:1118-1126]. Linear BCC was first described as a new clinical subtype in 1985 by Lewis [Int J Dematol 1985;24:124-125]. Here, we present a case of linear BCC that we recently encountered in an elderly Japanese patient, and review other cases reported in Japan
A Case of Syringomatous Adenoma of the Nipple
Syringomatous adenoma of the nipple (SAN) is an extremely rare disease originating in the adnexal gland; it was first reported by Rosen in 1983 [Am J Surg Pathol 1983;7:739–745]. Since then, 34 cases have been reported worldwide. We present the case of a 51-year-old man with SAN, in whom local excision of the tumor was performed. Histologically, the tumor consisted of tubules, ductules and epithelial cell strands, and most of the proliferating ducts presented with a characteristic teardrop or comma-shaped appearance
Adsorption, Desorption, Surface Diffusion, Lattice Defect Formation, and Kink Incorporation Processes of Particles on Growth Interfaces of Colloidal Crystals with Attractive Interactions
Good model systems are required in order to understand crystal growth processes because, in many cases, precise incorporation processes of atoms or molecules cannot be visualized easily at the atomic or molecular level. Using a transmission-type optical microscope, we have successfully observed in situ adsorption, desorption, surface diffusion, lattice defect formation, and kink incorporation of particles on growth interfaces of colloidal crystals of polystyrene particles in aqueous sodium polyacrylate solutions. Precise surface transportation and kink incorporation processes of the particles into the colloidal crystals with attractive interactions were observed in situ at the particle level. In particular, contrary to the conventional expectations, the diffusion of particles along steps around a two-dimensional island of the growth interface was not the main route for kink incorporation. This is probably due to the number of bonds between adsorbed particles and particles in a crystal; the number exceeds the limit at which a particle easily exchanges its position to the adjacent one along the step. We also found novel desorption processes of particles from steps to terraces, attributing them to the assistance of attractive forces from additionally adsorbing particles to the particles on the steps
Comparative analysis of photosynthetic properties in ice algae and phytoplankton inhabiting Franklin Bay, the Canadian Arctic, with those in mesophilic diatoms during CASES 03-04
Psychrophilic phytoplankton and ice algae were collected in Franklin Bay, the Canadian Arctic, in late May 2004, and the photosynthetic properties were measured at 4°C using a pulse amplitude modulation fluorometer (Phyto-PAM). Rapid light curve measurements allowed for the assessment of the photosynthetic efficiency (α), maximal electron transport rate (rETRmax), and minimum saturating irradiance (Ek) in the samples. The values of α in phytoplankton (0.63-0.68) were much larger than those in ice algae (0.10-0.51), and the values of rETRmax in phytoplankton (4.6-6.7) were relatively larger than those in ice algae (1.8-4.3). However, Ek showed similar values in both samples and were around 10μmol photonsm^・s^. These values were systematically compared with those obtained from mesophilic marine diatoms (a centric diatom, Chaetoceros gracilis, and a pennate diatom, Phaeodactylum tricornutum) grown under various irradiances in the laboratory. The highly shade-adapted features of ice algae and phytoplankton were disclosed through this comparative analysis. It was also found that the non-photochemical quenching was much higher in psychrophilic samples than in mesophilic diatoms grown under moderate irradiance. Furthermore, in ice algae and phytoplankton, the decrease in rETR at high irradiances was prominent, showing that they were highly susceptible to photoinhibition. Our comparative analysis using psychrophilic phytoplankton, ice algae and two strains of mesophilic diatoms also revealed that the dependency on the xanthophyll cycle for the protection mechanisms of photosystems were remarkably different between the groups, indicating that the acclimation strategies to growth irradiances were variable between species. Such variable acclimation strategies could be one of the forces that results in a diverse algal flora that enables this region around Franklin Bay to be a productive area, even though the psychrophilic phytoplankton and ice algae are highly shade-adapted
Human surfactant protein A with two distinct oligomeric structures which exhibit different capacities to interact with alveolar type II cells
Kinematic Structure of Molecular Gas around High-mass Star YSO, Papillon Nebula, in N159 East in the Large Magellanic Cloud
We present the ALMA Band 3 and Band 6 results of 12CO(2-1), 13$CO(2-1),
H30alpha recombination line, free-free emission around 98 GHz, and the dust
thermal emission around 230 GHz toward the N159 East Giant Molecular Cloud
(N159E) in the Large Magellanic Cloud (LMC). LMC is the nearest active
high-mass star forming face-on galaxy at a distance of 50 kpc and is the best
target for studing high-mass star formation. ALMA observations show that N159E
is the complex of filamentary clouds with the width and length of ~1 pc and 5
pc - 10 pc, respectively. The total molecular mass is 0.92 x 10^5 Msun from the
13CO(2-1) intensity. N159E harbors the well-known Papillon Nebula, a compact
high-excitation HII region. We found that a YSO associated with the Papillon
Nebula has the mass of 35 Msun and is located at the intersection of three
filamentary clouds. It indicates that the formation of the high-mass YSO was
induced by the collision of filamentary clouds. Fukui et al. 2015 reported a
similar kinematic structure toward a YSO in the N159 West region which is
another YSO that has the mass larger than 35 Msun in these two regions. This
suggests that the collision of filamentary clouds is a primary mechanism of
high-mass star formation. We found a small molecular hole around the YSO in
Papillon Nebula with sub-pc scale. It is filled by free-free and H30alpha
emission. Temperature of the molecular gas around the hole reaches ~ 80 K. It
indicates that this YSO has just started the distruction of parental molecular
cloud.Comment: 28 pages, 7 figures. Submitted to Ap
Alternative mRNA Splicing in Three Venom Families Underlying a Possible Production of Divergent Venom Proteins of the Habu Snake, Protobothrops flavoviridis
Snake venoms are complex mixtures of toxic proteins encoded by various gene families that function synergistically to incapacitate prey. A huge repertoire of snake venom genes and proteins have been reported, and alternative splicing is suggested to be involved in the production of divergent gene transcripts. However, a genome-wide survey of the transcript repertoire and the extent of alternative splicing still remains to be determined. In this study, the comprehensive analysis of transcriptomes in the venom gland was achieved by using PacBio sequencing. Extensive alternative splicing was observed in three venom protein gene families, metalloproteinase (MP), serine protease (SP), and vascular endothelial growth factors (VEGF). Eleven MP and SP genes and a VEGF gene are expressed as a total of 81, 61, and 8 transcript variants, respectively. In the MP gene family, individual genes are transcribed into different classes of MPs by alternative splicing. We also observed trans-splicing among the clustered SP genes. No other venom genes as well as non-venom counterpart genes exhibited alternative splicing. Our results thus indicate a potential contribution of mRNA alternative and trans-splicing in the production of highly variable transcripts of venom genes in the habu snake
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