Studies on Pig Erythrocyte Lipids (1). Fractionation of Erythrocyte Lipids by the Simple Combination of Silicic Acid and Cellulose Column chromatography.

1. The fractionation of pig erythrocytes lipids into the eight fractions, termed as S-0, S-2, S-5, S-10, S-20, S-25, S-40 and S-50 respectively, was carried out by silicic acid column chromatography, with determination of phosphorus and sugar contents in each fraction. The identification of lipids in each fraction was performed also by thin layer and silicic acid impregnated paper chromatography as well as infrared spectroscopy. 2. The chromatographic data showed that the fraction S-0 was composed mainly of cholesterol with a minor quantity of cholesterolester and/or monoglyceride, S-5 mainly of fatty acid, S-10 of cerebroside, cardiolipin and/or phosphatidic acid, S-20 of phosphatidyl-ethanolamine and phosphatidyl-serine, S-25 of phosphatidyl-ethanolamine and globoside, S-40 of phosphatidyl-choline and phosphatidyl-serine and S-50 of sphingomyelin with a minor quantity of phosphatidyl-serine and phosphatidyl-choline. 3. The last four fractions such as S-20, S-25, S-40 and S-50 were further separeted into four fractions (C-0, C-10, C-20 and C-50, respectively) by cellulose column chromatography with the determination of their phosphorus and sugar contents. The identification of the lipids in each fraction was performed by thin layer chromatography.4. The chromatographic data indicated that all the fractions C-0 derived from S-20, S-40 and S-50 were composed mainly of phosphatidyl-serine, while the fraction C-0 from S-25 mainly of globoside ; the fractions C-10 mainly of non-acidic phospholipids, that is, the fraction S-20 C-10 of phosphatidyl-ethanolamine, S-40 C-10 of phosphatidyl-choline, and S-50 C-10 of sphingomyelin ; the fraction C-50 mainly of globoside. 5. It was noted that phosphotidyl-serine wes eluted over wide range of fractions by silicic acid column chromatography, which was shown to be unsuitable for removal of phosphatidyl-serine from the common phospholipids, although phosphatidyl-serine could be well separated from the non-acdic phospholipids by cellulose column chromatography. The latter method was also utilizable for the separation of globoside from the other lipids. 6. The final identification of individual lipids will be presented in detail in the succeeding papers

ヒト扁桃腺濾胞でのB細胞分化の単クローン抗体を用いる免疫組織学的検討

雑誌掲載版免疫ペルオキシダーゼ法および,B1, B2, OKT9, OKT10, Leu-7などの単クローン抗体を用いて扁桃腺濾胞におけるB細胞分化について観察した.Mantle zoneはB1で濃くB2で軽度に染色されたが,germinal centerはB1, OKT10, OKT9で染色されB2でより濃く染色された.Germinal centerの中間層では,或る種の大型細胞がOKT10で染色された.Leu-7陽性細胞もgerminal center中間層に認められた.これらの成績をもとに,B細胞の分化について考察を加え