Students’ civil identity formation in multicultural educational space of school and tertiary institution

© 2015 Ashgate Publishing Ltd. All rights reserved. The relevance of the topic is determined by the search for the ways to improve one of the vital personality features, identity and civil identity as a driving motive in upbringing the younger generation. The aim of the paper is to reveal the content of the patriotic education model as a factor of forming civil identity of school and institute students being in multicultural education space and to define the organizing pedagogical conditions for its effective realization. The main approach to this problem research is competence approach which allows students to summon the acquired knowledge, skills, experience and ways of behavior in some definite situation or definite activity. The article may be useful for improvement the content and methods of educational work at educational institutes and the system of teachers’ professional development

NANOCRYSTALLINE CuS(II) SYNTHESIZED BY CHEMICAL BATH DEPOSITION

Nanocrystalline CuS was precipitated by using Na2S and thiocarbonic acid diamide N2H4CS and characterized by XRD analysis.Работа выполнена при финансовой поддержке Российского фонда фундаментальных исследований (Проект № 16-03-00566)

Towards an anti-fibrotic therapy for scleroderma: targeting myofibroblast differentiation and recruitment

BACKGROUND: In response to normal tissue injury, fibroblasts migrate into the wound where they synthesize and remodel new extracellular matrix. The fibroblast responsible for this process is called the myofibroblast, which expresses the highly contractile protein alpha-smooth muscle actin (alpha-SMA). In normal tissue repair, the myofibroblast disappears. Conversely, abnormal myofibroblast persistence is a key feature of fibrotic dieases, including scleroderma (systemic sclerosis, SSc). Myofibroblasts can be derived from differentiation of local resident fibroblasts or by recruitment of microvascular pericytes. CLINICAL PROBLEM ADDRESSED: Controlling myofibroblast differentiation and persistence is crucial for developing anti-fibrotic therapies targeting SSc. BASIC SCIENCE ADVANCES: Insights have been recently generated into how the proteins transforming growth factor beta (TGFbeta), endothelin-1 (ET-1), connective tissue growth factor (CCN2/CTGF) and platelet derived growth factor (PDGF) contribute to myofibroblast differentiation and pericyte recruitment in general and to the persistent myofibroblast phenotype of lesional SSc fibroblast, specifically. RELEVANCE TO CLINICAL CARE: This minireview summarizes recent findings pertinent to the origin of myofibroblasts in SSc and how this knowledge might be used to control the fibrosis in this disease. CONCLUSIONS: TGFbeta, ET-1, CCN2 and PDGF are likely to cooperate in driving tissue repair and fibrogenic responses in fibroblasts. TGFbeta, ET-1 and CCN2 appear to contribute to myofibroblast differentiation; PDGF appears to be involved with pericyte recruitment. Thus, different therapeutic strategies may exist for targeting the multisystem fibrotic disorder SSc

Activation of canonical Wnt signalling is required for TGF-β-mediated fibrosis

The transforming growth factor-β (TGF-β) signalling pathway is a key mediator of fibroblast activation that drives the aberrant synthesis of extracellular matrix in fibrotic diseases. Here we demonstrate a novel link between transforming growth factor-β and the canonical Wnt pathway. TGF-β stimulates canonical Wnt signalling in a p38-dependent manner by decreasing the expression of the Wnt antagonist Dickkopf-1. Tissue samples from human fibrotic diseases show enhanced expression of Wnt proteins and decreased expression of Dickkopf-1. Activation of the canonical Wnt pathway stimulates fibroblasts in vitro and induces fibrosis in vivo. Transgenic overexpression of Dickkopf-1 ameliorates skin fibrosis induced by constitutively active TGF-β receptor type I signalling and also prevents fibrosis in other TGF-β-dependent animal models. These findings demonstrate that canonical Wnt signalling is necessary for TGF-β-mediated fibrosis and highlight a key role for the interaction of both pathways in the pathogenesis of fibrotic diseases

Transcriptional Profiling of the Dose Response: A More Powerful Approach for Characterizing Drug Activities

The dose response curve is the gold standard for measuring the effect of a drug treatment, but is rarely used in genomic scale transcriptional profiling due to perceived obstacles of cost and analysis. One barrier to examining transcriptional dose responses is that existing methods for microarray data analysis can identify patterns, but provide no quantitative pharmacological information. We developed analytical methods that identify transcripts responsive to dose, calculate classical pharmacological parameters such as the EC50, and enable an in-depth analysis of coordinated dose-dependent treatment effects. The approach was applied to a transcriptional profiling study that evaluated four kinase inhibitors (imatinib, nilotinib, dasatinib and PD0325901) across a six-logarithm dose range, using 12 arrays per compound. The transcript responses proved a powerful means to characterize and compare the compounds: the distribution of EC50 values for the transcriptome was linked to specific targets, dose-dependent effects on cellular processes were identified using automated pathway analysis, and a connection was seen between EC50s in standard cellular assays and transcriptional EC50s. Our approach greatly enriches the information that can be obtained from standard transcriptional profiling technology. Moreover, these methods are automated, robust to non-optimized assays, and could be applied to other sources of quantitative data

Deletion of Wntless in myeloid cells exacerbates liver fibrosis and the ductular reaction in chronic liver injury

Background: Macrophages play critical roles in liver regeneration, fibrosis development and resolution. They are among the first responders to liver injury and are implicated in orchestrating the fibrogenic response via multiple mechanisms. Macrophages are also intimately associated with the activated hepatic progenitor cell (HPC) niche or ductular reaction that develops in parallel with fibrosis. Among the many macrophage-derived mediators implicated in liver disease progression, a key role for macrophage-derived Wnt proteins in driving pro-regenerative HPC activation towards a hepatocellular fate has been suggested. Wnt proteins, in general, however, have been associated with both pro-and anti-fibrogenic activities in the liver and other organs. We investigated the role of macrophage-derived Wnt proteins in fibrogenesis and HPC activation in murine models of chronic liver disease by conditionally deleting Wntless expression, which encodes a chaperone essential for Wnt protein secretion, in LysM-Cre-expressing myeloid cells (LysM-Wls mice)

Students’ civil identity formation in multicultural educational space of school and tertiary institution

© 2015 Ashgate Publishing Ltd. All rights reserved. The relevance of the topic is determined by the search for the ways to improve one of the vital personality features, identity and civil identity as a driving motive in upbringing the younger generation. The aim of the paper is to reveal the content of the patriotic education model as a factor of forming civil identity of school and institute students being in multicultural education space and to define the organizing pedagogical conditions for its effective realization. The main approach to this problem research is competence approach which allows students to summon the acquired knowledge, skills, experience and ways of behavior in some definite situation or definite activity. The article may be useful for improvement the content and methods of educational work at educational institutes and the system of teachers’ professional development

Students’ civil identity formation in multicultural educational space of school and tertiary institution

© 2015 Ashgate Publishing Ltd. All rights reserved. The relevance of the topic is determined by the search for the ways to improve one of the vital personality features, identity and civil identity as a driving motive in upbringing the younger generation. The aim of the paper is to reveal the content of the patriotic education model as a factor of forming civil identity of school and institute students being in multicultural education space and to define the organizing pedagogical conditions for its effective realization. The main approach to this problem research is competence approach which allows students to summon the acquired knowledge, skills, experience and ways of behavior in some definite situation or definite activity. The article may be useful for improvement the content and methods of educational work at educational institutes and the system of teachers’ professional development

Students’ civil identity formation in multicultural educational space of school and tertiary institution

© 2015 Ashgate Publishing Ltd. All rights reserved. The relevance of the topic is determined by the search for the ways to improve one of the vital personality features, identity and civil identity as a driving motive in upbringing the younger generation. The aim of the paper is to reveal the content of the patriotic education model as a factor of forming civil identity of school and institute students being in multicultural education space and to define the organizing pedagogical conditions for its effective realization. The main approach to this problem research is competence approach which allows students to summon the acquired knowledge, skills, experience and ways of behavior in some definite situation or definite activity. The article may be useful for improvement the content and methods of educational work at educational institutes and the system of teachers’ professional development