Isolation and identification of few fatty acid esters from the aerial roots of Rhaphidophora aurea twined over different host trees

Plants are the store houses of various secondary metabolites and bio-essential natural products. Several therapeutic agents are isolated from Rhaphidophora aurea. The aerial roots of the mentioned plant twined over different trees were explored. Sequential extraction (Polar to non-polar solvents) was carried out for the aerial roots of Rhaphidophora aurea twined over different host trees. Each extract was subjected to solvent-solvent fractionation. Through Thin-layer chromatography and column chromatography, the fatty acid compounds were identified and isolated. The isolated compounds were characterized through recording UV, IR, GC-MS/MS (Thermo), and 1D and 2D NMR techniques. Six fatty acids viz. 4-oxo-tricosanoic acid icosyl ester, butyl octadecanoate, dodecanoic acid dodec-3-enyl ester, octacosa-23, 26-dien-12-one, ethyl cis-6-octadecenoate and 15,18- dotriacontadienoic acid and methyl ester was isolated and characterized by analytical characterization techniques

Enhancing antibacterial efficacy and accelerating infectious wound healing in rats using biogenic metal nanoparticles from marine Bacillus subtilis

IntroductionMicroorganisms originating from the marine environment, such as bacteria, fungi, and algae, are deliberately employed in the production of nanoparticles on account of the wide array of bioactive compounds they produce.MethodsCell-free aqueous extracts of marine Bacillus subtilis (CBPPR1) were used to synthesise AuNPs (CBPPR1AuNPs) and AgNPs (CBPPR1AgNPs). Zetasizer Nano ZS (Malvern Instruments) zeta size and zeta potential, field emission and transmission scanning electron microscopy (FE-SEM and HR-TEM), UV-visible (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared (FT-IR), and EDAX were used to characterize biogenically synthesized nanoparticles (NPs). Their antibacterial activities against Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus were investigated. The anticancer efficacy of CBPPR1Au and AgNPs was investigated in human colorectal adenocarcinoma cell lines (HT-29, HT-116). CBPPR1AgNPs formulation was studied in vitro and in-vivo rat models. The assessment focused on its efficacy in wound healing and antibacterial capabilities, comparing them against a commercial product. To determine the effectiveness of CBPPR1AgNPs in wound healing, a cutaneous wound model was employed, which included infection with S. aureus.Results and discusionCBPPR1Au and AgNPs significantly inhibited the growth of S aureus at MIC of 125 µg (CBPPR1AuNPs) and 62.5 µg (CBPPR1AgNPs) respectively. FE-SEM and HR-TEM observations confirmed that NPs caused bacterial cell leakage, damage, and shrinkage. Cancer cell viability was reduced upon treatment with increasing concentrations of CBPPR1Au and AgNPs, and apoptosis was increased in cells treated with CBPPR1Au and AgNPs relative to untreated cells (p < 0.001). CBPPR1Au and AgNPs showed significant cytotoxic activity against HT-29 (15.5 M) and HT-116 (62.5 M) cells. In-vivo experiments on rats showed minimal pus formation in groups CBPPR1AgNPs (62.5 µg/ml) G2, CBPPR1AgNPs (125 µg/ml) G3, and silver sulfadiazine G4, indicating the effective control of infections. CBPPR1AgNPs-treated wounds showed complete closure, whereas untreated G1 wounds remained unhealed. Histopathological analysis showed no adverse effects of CBPPR1AgNPs on kidneys and livers of rats. These findings suggest that CBPPR1AgNPs play a pivotal role in wound healing because of their potent antibacterial properties

Isolation and identification of few fatty acid esters from the aerial roots of Rhaphidophora aurea twined over different host trees

234-243Plants are the store houses of various secondary metabolites and bio-essential natural products. Several therapeutic agents are isolated from Rhaphidophora aurea. The aerial roots of the mentioned plant twined over different trees were explored. Sequential extraction (Polar to non-polar solvents) was carried out for the aerial roots of Rhaphidophora aurea twined over different host trees. Each extract was subjected to solvent-solvent fractionation. Through Thin-layer chromatography and column chromatography, the fatty acid compounds were identified and isolated. The isolated compounds were characterized through recording UV, IR, GC-MS/MS (Thermo), and 1D and 2D NMR techniques. Six fatty acids viz. 4-oxo-tricosanoic acid icosyl ester, butyl octadecanoate, dodecanoic acid dodec-3-enyl ester, octacosa-23, 26-dien-12-one, ethyl cis-6-octadecenoate and 15,18- dotriacontadienoic acid and methyl ester was isolated and characterized by analytical characterization techniques

Magnetic synergism in Janus particles—Relevance to synthetic advances, materialistic properties and their niche applications

Multifunctional nanoparticles are emerging materials of interest as they outperform conventional nanoparticles. The advancing field of Janus nanoparticles relates to a dissymmetrical system with biphasic geometry and different chemical compositions. They combine the properties of two independent materials and so render superior properties than their symmetrical counterparts. Tailoring Janus materials with magnetic components find an extensive role in the biomedical to environmental applications owing to magnetic synergism. Unlike non-magnetic systems, Magnetic Janus particles (MJPs) facilitate facile separation, purification, recycling and complex superstructure formation, well-tuned by externally applied fields. The presence of magnetic components allows stimuli-responsive orientation, self-assembly, directionality and targeted site-specific action that make them appropriate candidates for targeted delivery and therapy, biomarkers, imaging, tracking and controlled-dynamics. This review article examines various MJP types, synthesis routes, anisotropy types, characterization methods, directed assembly, and related applications in relation to their magnetic behaviour. Along with the current difficulties and their potential solutions, the recent advancements in this area—such as the one-pot synthetic approach, biocompatible multifunctionalization, and combinatorial action as vectors, biomarkers, and theranostic agents—are highlighted

DataSheet_1_Enhancing antibacterial efficacy and accelerating infectious wound healing in rats using biogenic metal nanoparticles from marine Bacillus subtilis.docx

IntroductionMicroorganisms originating from the marine environment, such as bacteria, fungi, and algae, are deliberately employed in the production of nanoparticles on account of the wide array of bioactive compounds they produce.MethodsCell-free aqueous extracts of marine Bacillus subtilis (CBPPR1) were used to synthesise AuNPs (CBPPR1AuNPs) and AgNPs (CBPPR1AgNPs). Zetasizer Nano ZS (Malvern Instruments) zeta size and zeta potential, field emission and transmission scanning electron microscopy (FE-SEM and HR-TEM), UV-visible (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared (FT-IR), and EDAX were used to characterize biogenically synthesized nanoparticles (NPs). Their antibacterial activities against Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus were investigated. The anticancer efficacy of CBPPR1Au and AgNPs was investigated in human colorectal adenocarcinoma cell lines (HT-29, HT-116). CBPPR1AgNPs formulation was studied in vitro and in-vivo rat models. The assessment focused on its efficacy in wound healing and antibacterial capabilities, comparing them against a commercial product. To determine the effectiveness of CBPPR1AgNPs in wound healing, a cutaneous wound model was employed, which included infection with S. aureus.Results and discusionCBPPR1Au and AgNPs significantly inhibited the growth of S aureus at MIC of 125 µg (CBPPR1AuNPs) and 62.5 µg (CBPPR1AgNPs) respectively. FE-SEM and HR-TEM observations confirmed that NPs caused bacterial cell leakage, damage, and shrinkage. Cancer cell viability was reduced upon treatment with increasing concentrations of CBPPR1Au and AgNPs, and apoptosis was increased in cells treated with CBPPR1Au and AgNPs relative to untreated cells (p < 0.001). CBPPR1Au and AgNPs showed significant cytotoxic activity against HT-29 (15.5 M) and HT-116 (62.5 M) cells. In-vivo experiments on rats showed minimal pus formation in groups CBPPR1AgNPs (62.5 µg/ml) G2, CBPPR1AgNPs (125 µg/ml) G3, and silver sulfadiazine G4, indicating the effective control of infections. CBPPR1AgNPs-treated wounds showed complete closure, whereas untreated G1 wounds remained unhealed. Histopathological analysis showed no adverse effects of CBPPR1AgNPs on kidneys and livers of rats. These findings suggest that CBPPR1AgNPs play a pivotal role in wound healing because of their potent antibacterial properties.</p