Formulation and Evaluation of Polyherbal Antioxidant Face Cream Containing Ethanol Extracts of Psidium Guajava and Ocimum Gratissimum

Background: Aging is a natural progressive process that leads to aesthetic and functional changes in the skin. The aim of this research work is to formulate and evaluate a polyherbal antioxidant face cream using the ethanol extracts of psidium guajava and ocimum gratissimum.Method: The ethanol extract of the herbs was incorporated at varying concentrations into six different emulsion bases. Antioxidant activity of the formulations was assessed using 2, 2-Diphenyl-1-picrylhydrazyl method. The formulations were evaluated for pH, viscosity, spreadability and microbial content. Accelerated stability tests were performed on all the formulations to assess stability at varying storage conditions.Results: All the formulations showed good spread ability, good consistency, homogeneity, appearance, pH without phase separation occurring. Rheological tests showed that the all formulations exhibited non-Newtonian pseudo plastic flow. All six formulations also showed concentration dependent antioxidant activity. Ascorbic acid a potent antioxidant served as the standard for these tests. Formulation AFCR6 showed the highest antioxidant activity with IC50 value of 80.1 μg/mL.Conclusion: The polyherbal antioxidant cream containing extracts of Psidium guajava and Ocimum gratissimum have been shown to have excellent antioxidants properties. It can serve to protect the skin from reactive oxygen species created by UV radiation and environmental toxin, thus protecting the skin from photo aging.Keywords: Anti-oxidant, Face cream, Psidium guajava, Ocimum gratissimu

Modelling Crop Evapotranspiration and Water Use Efficiency of Maize Using Artificial Neural Network and Linear Regression Models in Biochar and Inorganic Fertilizer-Amended Soil under Varying Water Applications

The deficit irrigation strategy is a well-known approach to optimize crop water use through the estimation of crop water use efficiency (CWUE). However, studies that comprehensively reported the prediction of crop evapotranspiration (ETc) and CWUE under deficit irrigation for improved water resources planning are scarce. The objective of the study is to predict seasonal ETc and CWUE of maize using multiple linear regression (MLR) and artificial neural network (ANN) models under two scenarios, i.e., (1) when only climatic parameters are considered and (2) when combining crop parameter(s) with climatic data in amended soil. Three consecutive field experimentations were carried out with biochar applied at rates of 0, 3, 6, 10 and 20 t/ha, while inorganic fertilizer was applied at rates of 0 and 300 Kg/ha, under three water regimes: 100% Full Irrigation Treatment (FIT), 80% and 60% FIT. Seasonal ETc was determined using the soil water balance method, while growth data were monitored weekly. The CWUE under each treatment was also estimated and modelled. The MLR and ANN models were developed, and their evaluations showed that the ANN model was satisfactory for the predictions of both ETc and CWUE under all soil water conditions and scenarios. However, the MLR model without crop data was poor in predicting CWUE under extreme soil water conditions (60% FIT). The coefficient of determination (R2) increased from 0.03 to 0.67, while root mean-square error (RMSE) decreased from 4.07 to 1.98 mm after the inclusion of crop data. The model evaluation suggests that using a simple model such as MLR, crop water productivity could be accurately predicted under different soil and water management conditions

Genetic conservation through effective utilization of the improved indigenous chicken breeds by rural households in Nigeria

SUMMARY. Our collection and genetic characterization of various indigenous chicken genotypes in Nigeria started in 1984 at the National Animal Production Research Institute, Shika, Zaria, (Shika Brown) in 1994 at the Federal University of Agriculture, Abeokuta, Nigeria (FUNAAB) and in 2014 in Obafemi Awolowo University, Ile-Ife (FULANI). Collections were screened and characterized for genetic improvement and effective utilization by rural households in Nigeria With the financial and technical support of the Bill and Melinda Gates Foundation and the International Livestock Research Institute, Ethiopia, respectively, improved chicken genotypes were developed and evaluated alongside some other tropically adapted chicken breeds. A total of six improved tropically adapted chicken breeds (three Nigerian – Shika Brown, FUNAAB alpha and Fulani; three imported – Kuroiler, Sasso and Noiler) were tested for 52 weeks on-farm, across five agro-ecological zones of Nigeria and on-station in a public University farm and a private farm in Ogun and Oyo States, respectively. Results showed the Nigeria’s improved Indigenous chicken breed, the FUNAAB-Alpha, to be of comparable performance with the three imported breeds. It has met with high acceptance and demand after exhibition of the six breeds during the 2017 Science and Technology Expo in Abuja, Nigeria

Evaluating the Potentiating Effect of Amethocaine on Tropicamide-Induced Mydriasis in Darkly Pigmented Irides, Using Infrared Pupillometry

Purpose To determine whether prior instillation of amethocaine would increase the rate and magnitude of tropicamide-induced pupillary dilatation in darkly pigmented irides. Method A total of 50 healthy Africans aged 18–40 years, with darkly pigmented irides, received a drop of amethocaine in one eye and a drop of placebo in the contralateral eye, followed by two drops of tropicamide in both eyes. Serial pupil diameters were measured using a monocular infrared video pupillometer. Rate of pupillary dilatation was compared in both eyes. Survival statistics were calculated for the time taken to reach a clinically effective dilatation, clinically effective diameter (CED) of 6.0 mm. Results Mean difference in pupil diameters between amethocaine- and placebo-treated eyes was 0.30 ± 0.09 mm ( P < 0.002). In all, 62% of amethocaine-treated eyes and only 46% of placebo-treated eyes reached the CED. Conclusion We observed a small but statistically significant potentiating effect of a drop of amethocaine on tropicamide-induced dilatation within 20 minutes

Comparative genomic analyses of β-lactamase (<i>bla_CMY-42</i>)-encoding plasmids isolated from wastewater treatment plants in Canada

Wastewater treatment plants are useful environments for investigating the occurrence, diversity, and evolution of plasmids encoding clinically relevant antibiotic resistance genes. Our objective was to isolate and sequence plasmids encoding meropenem resistance from bacterial hosts within Canadian WWTPs. We used two enrichment culture approaches for primary plasmid isolation, followed by screening of antibiotic resistance, conjugative mobility, and stability in enteric bacteria. Isolated plasmids were sequenced using Illumina MiSeq and Sanger sequencing methods. Bioinformatics analyses resolved a multi-resistance IncF/MOBF12 plasmid, pFEMG (209,357 bp), harbouring resistance genes to beta-lactam (blaCMY-42, blaTEM-1β, and blaNDM-5), macrolide (mphA-mrx-mphR), tetracycline (tetR-tetB-tetC-tetD), trimethoprim (dfrA12), aminoglycoside (aadA2), and sulfonamide (sul1) antibiotic classes. We also isolated an IncI1/MOBP12 plasmid pPIMR (172,280 bp), carrying similar beta-lactamase and a small multidrug efflux resistance gene cluster (blaCMY-42-blc-sugE) to pFEMG. The co-occurrence of different ARGs within a single 24,552 bp cluster in pFEMG – intersperced with transposons, insertion sequence elements, and a class 1 integron – maybe of significant interest to human and veterinary medicine. Additionally, the presence of conjugative and plasmid maintenance genes in the studied plasmids corresponds to the observed high conjugative transfer frequencies and stable maintenance. Extensive investigation is required to further understand the fitness trade offs of plasmids having differing types of conjugative transfer and maintenance modules.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Predictors and outcome of acute kidney injury after non-cardiac paediatric surgery

Abstract Background It is necessary to define the problem of acute kidney injury (AKI) after non-cardiac surgery in order to design interventions to prevent AKI. The study aimed to evaluate the occurrence, determinants and outcome of AKI among children undergoing general (non-cardiac) surgery. Methods This was a prospective cohort study of patients aged ≤ 15 years who had general surgery over 18 months period at a tertiary hospital in Nigeria. AKI was evaluated at 6 and 24 h and within 7 days of surgery. Data were analysed using SPSS version 21. Results A total of 93 patients were studied with age ranging from 3 days to 15 years (median = 4 years). AKI occurred within 24 h of surgery in 32 (34.4%) and cumulatively over 7 days in 33 (35.5%). Patients who had sepsis were nearly four times as likely as others to develop perioperative AKI (OR = 3.52, 95% CI 1.21, 10.20, p = 0.021). Crude mortality rate was 12.1% (4/33); no mortality was recorded among those without AKI, p = 0.014. Conclusion Perioperative AKI occurred in 35.5% of children who underwent general (non-cardiac) surgery. Patients who had sepsis were four times more likely than others to develop AKI. Mortality was documented only in patients who had AKI

COVID-19 Convalescent Plasma Therapy: Long Term Implications

Abstract Background The long-term effect of Coronavirus Disease 2019 (COVID-19) acute treatments on post-acute sequelae of SARS-CoV-2 infection (PASC) is unknown. The CONTAIN-Extend study explores the long-term impact of COVID-19 convalescent plasma (CCP) therapy on Post-Acute Sequelae of SARS-CoV-2 infection (PASC) symptoms and general health 18 months following hospitalization. Methods The CONTAIN-Extend study examined 281 participants from the original CONTAIN COVID-19 trial (CONTAIN-RCT, NCT04364737) at 18 months post-hospitalization for acute COVID-19. Symptom surveys, global health assessments, and biospecimen collection was performed from November 2021 to October 2022. Multivariable logistic and linear regression estimated associations between the randomization arms and self-reported symptoms and PROMIS scores, adjusted for covariables, including age, sex, race/ethnicity, disease severity, and CONTAIN enrollment quarter and sites. Results There were no differences in symptoms or PROMIS scores between CCP and placebo (adjusted odds ratio of general symptoms, 0.95; 95% confidence intervals, 0.54, 1.67). However, females (3.01; 1.73, 5.34), those 45-64 years (2.55; 1.14, 6.23), and April-June 2020 enrollees (2.39; 1.10, 5.19) were more likely to report general symptoms and have poorer PROMIS physical health scores than their respective reference groups. Hispanic participants (difference, -3.05; 95% CI, -5.82, -0.27) and Black participants (-4.48; -7.94, -1.02) had poorer PROMIS physical health than White participants. Conclusions CCP demonstrated no lasting effect on PASC symptoms or overall health in comparison to the placebo. This study underscores the significance of demographic factors, including sex, age, and the timing of acute infection, in influencing symptom reporting 18 months after acute hypoxic COVID-19 hospitalization