A review of the biochemical, biotechnological and other applications of enzymes

Enzymes are proteins evolved by the cells of living organisms. Their specific function is to catalyse chemical reactions. Enzymes increase the rate at which reactions approach equilibrium. Enzymes have found wide and diverse applications in different disciplines and fields of human endeavours. Enzymesplay critical role in the metabolic activities of all living organisms, whether humans, animals, plants or microorganisms and are widely applied in agriculture, biochemistry, biotechnology, chemistry, genetics, industries, human and veterinary medicine, pharmacy, research, etc. The differentapplications of enzymes abound in the literature. The aim of this review is to discuss the diverse applications of enzymes for the purpose of teaching, education and research

Evaluation of the antimicrobial activity of saponins extract of Sorghum Bicolor L. Moench

The n-butanol purified saponin extract of sorghum bicolor were screened for anti-bacterial activity against three pathogenic microbes; Escherichia coli, Staphylococcus aureus and Candida albicans. The extract inhibited the growth of the S. aureus. It was concluded that the saponins have inhibitory effect on gram-positive organism but not on gram negative organism and the fungi

Evaluation of organic materials for ingibition of nematode reproduction in soybean

Synthetic pesticides have been found to increase the yield of agricultural products over tenfold. However, the effects of pesticide misuse around the world include costly environmental pollution and disruption of the balance of nature in addition to their high cost and non-availability. Hence the need for search for alternative pesticideformulations to avert these undesirable effects. A study was conducted to evaluate the efficacy of Neem (Azadirachta indica A. Juss) leaf powder and organic fertiliser for management of M. incognita in soybean(Glycine max) production. A screenhouse experiment was conducted to test the efficacy of neem leaf powder (NLP) and organic based fertiliser (OBF), alone and a combination of 50% each of the NLP and OBF against root galling and Meloidogyne incognita multiplication on soybean and their potency in grain yield improvement. Theexperiment was laid out in completely randomised design with three replications. Although all treatments were effective in reducing root galling and nematode reproduction, NLP applied alone gave the highest reduction (92.59 %) in gall formation and nematode multiplication (82.07 %), followed by NLP + OBF and then OBF alone. Increases in grain yield ranged from 103.08 to 108.46% in response to organic soil amendment. Integration of the two organic substances is a better and more promising strategy since it reduced the quantity of organicfertiliser that had to be transported to farmer’s field by 50%

Antibacterial and antifungal activities of methanol extracts of Desmodium adscendens root and Bombax buonopozense leaves

In-vitro antibacterial and antifungal activities of methanol extracts of Desmodium adscendens (Sw.) DC root and Bombax buonopozense P. Beauv. leaves against some bacterial and fungal isolates implicated in oro-dental, urogenital and other opportunistic infections were investigated using standard microbiological methods. The extracts of D. adscendens exhibited significant inhibitory action against all twelve organisms tested at a concentration of 2.00 mg/ml, while that of B. buonopozense inhibited nine organisms at the same concentration and conditions. The minimum inhibitory concentration (MIC) exhibited by extract of D. adscendens ranged between 0.0625 and 0.25 mg/ml while that of B. buonopozense ranged between 0.125 and 0.50 mg/ml. Phytochemical analysis of the two plant extracts revealed the presence of tannins and saponins while D. adscendens has alkaloids and flavonoids in addition. Between 92 and 100% of Staphylococcus aureus SA 1199 were killed within 120 minutes of contact time with a concentration of 0.25 - 0.50 mg/ml, while 98 - 100% of Candida albicans ATCC 90029 were killed within the same period and  concentration by the same extract (D. adscendens). Therefore, this study justified the antimicrobial properties of these plants, hence the plants could be recommended for use as source of new drugs for fungal and bacterial infections. © 2013 International Formulae Group. All rights reserved.Keywords: Desmodium adscendens, Bombax buonopozense, antibacterial, antifungal, Minimum Inhibitory Concentratio

EVALUATION OF PLANTING PERIODS AND FERTILIZER TYPES ON GROWTH AND YIELD OF CUCUMBER INTERCROPPED WITH PAWPAW VARIETIES

In a two phased field experiment conducted between 2006 and 2008 at the University of Agriculture, Abeokuta, the performances of cucumber (Cucumber sativus L.) var. Market-more introduced into pawpaw (Carica papaya L.) var: ‘Homestead selection’ and ‘Sunrise solo’ at the early, simultaneous and late times of introduction using 10 t/ha OMF as basal application was evaluated. Also in the mixture, the best fertilizer type involving 10 t/ha OMF or NPK 15:15:15 at 125 kg / ha / month using the zero (0 t/ha) application as control was determined. The factorial experiment was arranged in a randomized complete block design replicated three times. The results indicated that except at early cucumber introduction, growth and yield depression occurred in the crop mixture compared to the sole in the main vine length, leaf area, number of fruits, fruit yield and relative yield total (RYT). The cucumber performance was better in juvenile pawpaw (one year old) than in mature pawpaw (two year old). Both early and simultaneous introductions under juvenile and mature pawpaw were significantly better than late introduction. The cucumber with OMF was higher than those with NPK treatment in main vine length (101.6 cm and 53.3 cm respectively) and both were significantly better than the control (38.6 cm), though plants with OMF and NPK both were not different in leaf area (4,844 and 4,874 cm2 respectively), while plants with NPK compared to OMF recorded higher number of fruits (16 and 14 respectively) and higher fruit yield (13.8 and 11.2 t/ha respectively). The LER > 1.0 recorded for both cucumbers in Sunrise or Homestead indicated yield advantages of the mixtures compared to the sole.Â

THE INFLUENCE OF TYPE OF CUTTING AND GROWING MEDIUM ON GROWTH AND FLOWERING OF SCORPION ORCHID (Arachnis maingayi) Hook.f. Schltr

Orchids are of importance primarily for their horticultural appeal and accounts for 8% of the world floricultural trade. Scorpion orchid (Arachnis maingayi Hook) is highly prized for its beautiful long lasting flowers, but it is under-exploited in many countries including Nigeria. There is the need to determine the best type of cutting and growing medium protocols for commercial production of this species. Seven homogenous growing media (sawdust, topsoil, charcoal, coconut husk, wood shavings, maize cobs and rice husk) and two types of cutting (apical and non-apical) were compared in a factorial experiment laid out in Completely Randomized Design with six replications at the Federal University of Agriculture, Abeokuta, Nigeria between 2010 and 2012. Data collected on plant height, number of leaves, leaf area, shoot dry weight, days to spiking, length and number of spike, size and number of florets were subjected to analysis of variance and Tukey’s HSD at p ≤ 0.05 to separate treatment means. Apical cuttings were significantly (p ≤ 0.05) superior to non-apical cuttings in terms of all parameters assessed except floret diameter which was statistically similar. For growing medium, dry matter accumulation and spike yield were in the order of charcoal > rice husk > maize cob > topsoil > wood shavings > coconut husk. Apical cuttings planted in charcoal or rice husk gave the best results relative to other media. Thus, apical cutting is the best propagating technique and charcoal or rice husk is the best growing medium for scorpion orchid cultivation. &nbsp

Cytotoxic Activity of Crude Extracts as well as of Pure Components from Jatropha Species, Plants Used Extensively in African Traditional Medicine

Extracts from Jatropha curcas, a plant used in African traditional medicine for various diseases, were tested for cytotoxic activity. The root extracts strongly reduced cell growth of tumor cells in vitro, a result consistent with the knowledge of the application of these plant extracts in traditional medicine, especially to cure/ameliorate cancer. A selection of pure diterpenoids existing in extracts from Jatropha species and isolated from J. curcas, for example, curcusone C, curcusone D, multidione, 15-epi-4Z-jatrogrossidentadion, 4Z-jatrogrossidentadion, 4E-jatrogrossidentadion, 2-hydroxyisojatrogrossidion, and 2-epi-hydroxyisojatrogrossidion, were likewise tested, and they also showed strong cytotoxic activity. It turned out that these extracts are highly active against L5178y mouse lymphoma cells and HeLa human cervix carcinoma cells, while they cause none or only very low activity against neuronal cell, for example, PC12. These data underscore that extracts from J. curcas or pure secondary metabolites from the plant are promising candidates to be anticancer drug, combined with low neuroactive effects

GC-MS Analysis, Antimicrobial and Antioxidant Activities of Extracts of the Aerial Parts of Conyza sumatrensis

Phytochemical analyses as well as antimicrobial and antioxidant activities of the extracts of C. sumatrensis aerial parts were investigated in this study. METHODS: The aerial parts of C. sumatrensis were air dried, weighed and exhaustively extracted with hexane, ethyl acetate and methanol successively. The crude extracts were screened for metabolites.  These extracts of the plant were evaluated for antimicrobial and antioxidant activities using agar  diffusion and DPPH method respectively. The extracts were also analysed using Gas chromatography – Mass spectrometry, and the chromatogram coupled with mass spectra of the compounds were matched with a standard library. RESULTS: Preliminary phytochemical investigation of  rude n-hexane, ethyl acetate and methanol extracts of the aerial parts of Conyza sumatrensis revealed the presence of anthraquinones, flavonoids, terpenoids, phenolics, tannin, glycosides and carbohydrate. All the crude  extracts gave a clear zone of inhibition against the growth of the test bacteria (Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomona aeruginosa, Salmonella typhi, Klebsiellae pneumonae) at moderate to high  concentrations, as well as test fungi (Candida albicans, Aspergillus niger, penicillium notatum and  Rhizopus stolonifer) at high concentration. Methanolic extract exhibited significant radical scavenging  property with IC50 of 17.08 μg/mL while n-hexane and ethyl acetate extracts showed no significant antioxidant activity. GC-MS of N-hexane extract showed a total number of eleven chemical constituents with α-Farnesene and spathulenol being the most abundance compounds constituting 20.27 and 22.28% of the extract respectively. Ethyl acetate extract revealed thirteen  compounds with two most abundant compounds, cis-β-farnesene (16.64 %) and cis-pinane (21.09 %). While  methanolic extract affords seventeen compounds with Ephytol being the most abundant compound  (19.36 %). © JASEMKeywords: Antimicrobial activity, Antioxidant, GC-MS analysis, Phytochemicals, Conyza sumatrensi