Oriented Fibers Cooperate with DFO to Prevent Tendon Adhesions by Improving the Repair Microenvironment

Abstract To solve the problem of tendon adhesion after an operation, an anti‐adhesion membrane is designed, which can inhibit the exogenous healing of tendons and promote endogenous healing. Here, poly[3(S)‐methyl‐morpholine‐2,5‐dione‐co‐lactic acid] P(MMD‐co‐LA) containing alanine units is obtained by melt ring‐opening polymerization (ROP). It can effectively reduce the production of acidic degradation products that cause aseptic inflammation. Then a kind of oriented P(MMD‐co‐LA)/deferoxamine (DFO) anti‐adhesion membrane with good mechanical properties, cell adhesion properties, and induced macrophage polarization properties is constructed by electrospinning. In vitro and in vivo studies have shown that oriented fibrous membranes can down‐regulate the expression of inflammatory cytokines. In addition, the fibrous membrane can up‐regulate the expression of angiogenesis‐related genes, namely HIF1‐α, SDF‐1α, and VEGF, by releasing DFO in situ, thus promoting the revascularization of tendon defects and providing nutritional support for endogenous tendon healing. This method provides a new barrier strategy to reduce tendon adhesion through anti‐inflammation combined with vascularization to inhibit exogenous tendon healing while promoting tendon endogenous healing. The results provide essential insights into the corresponding regulation of the tendon healing microenvironment

Characterization of the PH1704 protease from Pyrococcus horikoshii OT3 and the critical functions of Tyr120.

The PH1704 protease from hyperthermophilic archaean Pyrococcus horikoshii OT3 is a member of DJ-1/ThiJ/PfpI superfamily with diverse functional subclasses. The recombinant PH1704 was efficiently purified and was systematically characterized by a combination of substrate specificity analysis, steady-state kinetics study and molecular docking research. The homogeneous protease was obtained as a presumed dodecamer with molecular weight of ∼240 kDa. Iodoacetamide strongly inhibited the peptidase activity, confirming that Cys100 is a nucleophilic residue. The recombinant protein was identified as both an aminopeptidase and an endopeptidase. Experimental data showed that L-R-amc was the best substrate of PH1704. Structural interaction fingerprint analysis (SIFt) indicated the binding pose of PH1704 and showed that Tyr120 is important in substrate binding. Kinetic parameters Kcat and Kcat/Km of the Y120P mutant with L-R-amc was about 7 and 7.8 times higher than that of the wild type (WT). For the endopeptidase Y120P with AAFR-amc, Kcat and Kcat/Km is 10- and 21-fold higher than that of WT. Experimental data indicate the important functions of Tyr120: involvement in enzyme activity to form a hydrogen bond with Cys100 and as an entrance gate of the substrate with Lys43. The results of this study can be used to investigate the DJ-1/ThiJ/PfpI superfamily

Fire Performance And Post-Fire Mechanical Properties Of Polymer Composites Coated With Hybrid Carbon Nanofiber Paper

Recent research results have failed to support the conventionally held belief that students learn physics best from hands-on experiences with physical equipment. Rather, studies have found that students who perform similar experiments with computer simulations perform as well or better on measures of conceptual understanding than their peers who used physical equipment. In this study, we explored how university-level nonscience majors\u27 understanding of the physics concepts related to pulleys was supported by experimentation with real pulleys and a computer simulation of pulleys. We report that when students use one type of manipulative (physical or virtual), the comparison is influenced both by the concept studied and the timing of the post-test. Students performed similarly on questions related to force and mechanical advantage regardless of the type of equipment used. On the other hand, students who used the computer simulation performed better on questions related to work immediately after completing the activities; however, the two groups performed similarly on the work questions on a test given one week later. Additionally, both sequences of experimentation (physical-virtual and virtual-physical) equally supported students\u27 understanding of all of the concepts. These results suggest that both the concept learned and the stability of learning gains should continue to be explored to improve educators\u27 ability to select the best learning experience for a given topic. Published by the American Physical Society under the terms of the Creative Commons Attribution 3.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article\u27s title, journal citation, and DOI

Effect of La3+ and Mg2+ combined system on bioactivity and osteogenesis of bioinspired La-doped magnesium phosphate composites prepared utilizing the precursor method

Lanthanum (La) compounds have attracted much attention because of low toxicity and biocompatibility. In this study, a series of La-doped magnesium phosphate composites bone repair materials (LMPCs) were prepared by the precursor method, in which the molar ratio of lanthanum was 1%, 3% and 5%, respectively. The results of X-ray powder diffraction, scanning electron microscope and compressive strength test show that La-doped magnesium phosphates (LMPs) synthesized by the precursor method have uniform particle distribution, high purity and excellent degradability. In vitro research results show that LMPCs exhibited reliable biosafety and excellent bioactivity. The introduction of lanthanum enhances the antibacterial activity of Escherichia coli and Staphylococcus aureus of the composite. Furthermore, the combined system of released La3+ and Mg2+ is more conducive to promoting the proliferation of mesenchymal stromal cells (MSC) and angiogenesis of human umbilical vein endothelial cells (HUVECs) when the content of lanthanum is 3% (referred to as LMPC-2). By up-regulating the expression of alkaline phosphatase (ALP) and Runt-related transcription factor 2 (RUNX2), LMPC-2 is prominent in promoting osteogenic differentiation, and mineralized calcium deposition in vitro. Moreover, the results of micro-CT, H&E and Masson staining confirmed the beneficial effect of the combined system of La3+ and Mg2+ on the repair of rat skull defects in vivo. These achievements will provide new strategies for improving the biological activity of LMPCs and provide reference value for bone repairing in the clinic

Exploration of Affectionate Care Services for Empty Nesters in the Context of “Internet +”

The study found that the number of empty nesters in China will increase to more than 200 million in 2030. It accounts for 90% of the total number of elderly people, and the elderly in China will have an “empty nesting” situation. In the era when empty nesters are becoming more and more serious, the mental health of empty nesters is becoming increasingly prominent, which has become a key topic of concern in the whole society. In order to better develop the physical and mental health of the elderly, in the context of Internet+, this article uses SWOT method to analyze the issue of family care services for empty nesters. During the discussion, it was found that there are promotion problems, platform security problems, page operation problems and personnel needs problems in the family care service. From these four aspects, the article gives four suggestions of “increasing publicity, strengthening and improving network security, improving the service function of the platform and paying attention to personnel needs”

White OLED Weibull life prediction using maximum likelihood estimation

A theoretical model using Weibull distribution and maximum likelihood estimation (MLE) was established to statistically analyze the test data, which were obtained by three groups of constant stress accelerated life tests. The life prediction software was applied to simplify the calculation and achieve organic light-emitting device (OLED) life estimation. The results indicate that the Weibull distribution is fit to describe white OLED life, and the precise accelerated parameter beta is particularly useful to predict the white OLED life within a shorter time, which provides significant guidelines to help engineers make decisions in design and manufacturing strategy from the aspect of reliability life

Risk of bias of included studies.

<p>Risk of bias of included studies.</p

General characteristics of included studies.

<p>−/−: ultrasound-guided injection/palpation-guided injection. BMI: body mass index; RCT: randomization controlled trial; VAS: visual analogue score; TT: tenderness threshold; HTI: heel tenderness index; PFT: plantar fascia thickness; HPT: heel pad thickness; RR: response rate.</p

Forest plot for hypoechogenicity between ultrasound-guided injection and palpation-guided injection.

<p>Forest plot for hypoechogenicity between ultrasound-guided injection and palpation-guided injection.</p

Forest plot for plantar fascia thickness between ultrasound-guided injection and palpation-guided injection.

<p>Forest plot for plantar fascia thickness between ultrasound-guided injection and palpation-guided injection.</p