The chromokinesin Kid is necessary for chromosome arm orientation and oscillation, but not congression, on mitotic spindles

Chromokinesins have been postulated to provide the polar ejection force needed for chromosome congression during mitosis. We have evaluated that possibility by monitoring chromosome movement in vertebrate-cultured cells using time-lapse differential interference contrast microscopy after microinjection with antibodies specific for the chromokinesin Kid. 17.5% of cells injected with Kid-specific antibodies have one or more chromosomes that remain closely opposed to a spindle pole and fail to enter anaphase. In contrast, 82.5% of injected cells align chromosomes in metaphase, progress to anaphase, and display chromosome velocities not significantly different from control cells. However, injected cells lack chromosome oscillations, and chromosome orientation is atypical because chromosome arms extend toward spindle poles during both congression and metaphase. Furthermore, chromosomes cluster into a mass and fail to oscillate when Kid is perturbed in cells containing monopolar spindles. These data indicate that Kid generates the polar ejection force that pushes chromosome arms away from spindle poles in vertebrate-cultured cells. This force increases the efficiency with which chromosomes make bipolar spindle attachments and regulates kinetochore activities necessary for chromosome oscillation, but is not essential for chromosome congression

Aerosol Extinction Uncertainty Determination for a Laser-Based Detecting System

Quantitative measurement of optic and aerosolic property of troposphere may be carried out from lidar system more easily and with low marginal cost than other system (i.e. satellite observation). The aim of this work is to show how extinction coefficient and water vapor concentration in troposphere may be educed from lidar signals at several altitudes and how uncertainty is close to acceptable value. Real data observations will be presented to demostrate uncertainty range on aerosolic extinction coefficient function

Optimization of Apodized Pupil Lyot Coronagraph for ELTs

We study the optimization of the Apodized Pupil Lyot Coronagraph (APLC) in the context of exoplanet imaging with ground-based telescopes. The APLC combines an apodization in the pupil plane with a small Lyot mask in the focal plane of the instrument. It has been intensively studied in the literature from a theoretical point of view, and prototypes are currently being manufactured for several projects. This analysis is focused on the case of Extremely Large Telescopes, but is also relevant for other telescope designs. We define a criterion to optimize the APLC with respect to telescope characteristics like central obscuration, pupil shape, low order segment aberrations and reflectivity as function of the APLC apodizer function and mask diameter. Specifically, the method was applied to two possible designs of the future European-Extremely Large Telescope (E-ELT). Optimum configurations of the APLC were derived for different telescope characteristics. We show that the optimum configuration is a stronger function of central obscuration size than of other telescope parameters. We also show that APLC performance is quite insensitive to the central obscuration ratio when the APLC is operated in its optimum configuration, and demonstrate that APLC optimization based on throughput alone is not appropriate.Comment: 9 pages, 17 figures, accepted for publication in Astronomy & Astrophysic

Formation, Simulation and Restoration of Hypertelescopes Images

This book is a collection of 19 articles which reflect the courses given at the Collège de France/Summer school “Reconstruction d'images − Applications astrophysiques“ held in Nice and Fréjus, France, from June 18 to 22, 2012. The articles presented in this volume address emerging concepts and methods that are useful in the complex process of improving our knowledge of the celestial objects, including Earth

On-sky observations with an achromatic hybrid phase knife coronagraph in the visible

CONTEXT: The four-quadrant phase mask stellar coronagraph, introduced by D. Rouan et al., is capable of achieving very high dynamical range imaging and was studied in the context of the direct detection of extra-solar planets. Achromatic four-quadrant phase mask is currently being developed for broadband IR applications. AIMS: We report on laboratory and on-sky tests of a prototype coronagraph in the visible. This prototype, the achromatic hybrid phase knife coronagraph, was derived from the four-quadrant phase mask principle. METHODS: The instrumental setup implementing the coronagraph itself was designed to record the pre- and post-coronagraphic images simultaneously so that an efficient real-time image selection procedure can be performed. We describe the coronagraph and the associated tools that enable robust and repeatable observations. We present an algorithm of image selection that has been tested against the real on-sky data of the binary star HD80081 (* 38 Lyn). RESULTS Although the observing conditions were poor, the efficiency of the proposed method is proven. From this experiment, we derive procedures that can apply to future focal instruments associating adaptive optics and coronagraphy, targeting high dynamic range imaging in astronomy, such as detecting extra-solar planets

Adaptive optics imaging of P Cygni in Halpha

We obtained Halpha diffraction limited data of the LBV star P Cyg using the ONERA Adaptive Optics (AO) facility BOA at the OHP 1.52m telescope on October 1997. Taking P Cyg and the reference star 59 Cyg AO long exposures we find that P Cyg clearly exhibits a large and diffuse intensity distribution compared to the 59 Cyg's point-like source. A deconvolution of P Cyg using 59 Cyg as the Point Spread Function was performed by means of the Richardson-Lucy algorithm. P Cyg clearly appears as an unresolved star surrounded by a clumped envelope. The reconstructed image of P Cyg is compared to similar spatial resolution maps obtained from radio aperture synthesis imaging. We put independent constraints on the physics of P Cyg which agree well with radio results. We discuss future possibilities to constrain the wind structure of P Cyg by using multi-resolution imaging, coronagraphy and long baseline interferometry to trace back its evolutionary status.Comment: 10 pages, 19 Encapsulated Postscript figure

Speckle Statistics in Adaptively Corrected Images

(abridged) Imaging observations are generally affected by a fluctuating background of speckles, a particular problem when detecting faint stellar companions at small angular separations. Knowing the distribution of the speckle intensities at a given location in the image plane is important for understanding the noise limits of companion detection. The speckle noise limit in a long-exposure image is characterized by the intensity variance and the speckle lifetime. In this paper we address the former quantity through the distribution function of speckle intensity. Previous theoretical work has predicted a form for this distribution function at a single location in the image plane. We developed a fast readout mode to take short exposures of stellar images corrected by adaptive optics at the ground-based UCO/Lick Observatory, with integration times of 5 ms and a time between successive frames of 14.5 ms ($\lambda=2.2$ $\mu$m). These observations temporally oversample and spatially Nyquist sample the observed speckle patterns. We show, for various locations in the image plane, the observed distribution of speckle intensities is consistent with the predicted form. Additionally, we demonstrate a method by which $I_c$ and $I_s$ can be mapped over the image plane. As the quantity $I_c$ is proportional to the PSF of the telescope free of random atmospheric aberrations, this method can be used for PSF calibration and reconstruction.Comment: 7 pages, 4 figures, ApJ accepte

XNAT-PIC: Extending XNAT to Preclinical Imaging Centers

Molecular imaging generates large volumes of heterogeneous biomedical imagery with an impelling need of guidelines for handling image data. Although several successful solutions have been implemented for human epidemiologic studies, few and limited approaches have been proposed for animal population studies. Preclinical imaging research deals with a variety of machinery yielding tons of raw data but the current practices to store and distribute image data are inadequate. Therefore, standard tools for the analysis of large image datasets need to be established. In this paper, we present an extension of XNAT for Preclinical Imaging Centers (XNAT-PIC). XNAT is a worldwide used, open-source platform for securely hosting, sharing, and processing of clinical imaging studies. Despite its success, neither tools for importing large, multimodal preclinical image datasets nor pipelines for processing whole imaging studies are yet available in XNAT. In order to overcome these limitations, we have developed several tools to expand the XNAT core functionalities for supporting preclinical imaging facilities. Our aim is to streamline the management and exchange of image data within the preclinical imaging community, thereby enhancing the reproducibility of the results of image processing and promoting open science practices